Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats
Gene expression and post-transcriptional regulation are key mechanisms affecting lactation performance in dairy animals. However, the difficulty of obtaining mammary gland tissue samples from lactating animals has significantly impeded lactation research. Milk fat globules may be a non-invasive way...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Food Chemistry: Molecular Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666566225000048 |
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| author | Hancai Jiang Xiaoxian Xu Shuwan Wang Xinhui Song Ling Li Qingyou Liu Kuiqing Cui Deshun Shi Jian Wang Hui Li Jieping Huang Zhipeng Li |
| author_facet | Hancai Jiang Xiaoxian Xu Shuwan Wang Xinhui Song Ling Li Qingyou Liu Kuiqing Cui Deshun Shi Jian Wang Hui Li Jieping Huang Zhipeng Li |
| author_sort | Hancai Jiang |
| collection | DOAJ |
| description | Gene expression and post-transcriptional regulation are key mechanisms affecting lactation performance in dairy animals. However, the difficulty of obtaining mammary gland tissue samples from lactating animals has significantly impeded lactation research. Milk fat globules may be a non-invasive way to obtain mammary transcripts. Here, we aimed to reveal the universal rule of the transcript profiles of the milk fat globules and mammary glands from buffaloes and goats by RNA-sequencing analysis. Results showed that, in buffalo, 97 % of mRNAs were expressed in both milk fat globules and mammary glands, with 45 % showing differential expression. Among 6086 lncRNAs and 7010 miRNAs identified, 35 % and 50 % were differentially expressed, respectively. Of 11,631 circRNAs, only 618 showed significant differences. In goat, more than 99 % of mRNAs and 87 % of ncRNAs (including lncRNAs, circRNAs, and miRNAs) were expressed in both milk fat globules and mammary glands, and over 91 % of mRNAs, 96 % of lncRNAs, 98 % of circRNAs, and 86 % of miRNAs showed no significant differences, suggests that the transcripts in milk fat globules exactly reflect that in the mammary gland. This study suggests that milk fat globules are an effective candidate for non-invasive acquisition of mammary gland transcripts, but their applicability needs further study. |
| format | Article |
| id | doaj-art-4560a5da6a0e4838945380cd4812402b |
| institution | Kabale University |
| issn | 2666-5662 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: Molecular Sciences |
| spelling | doaj-art-4560a5da6a0e4838945380cd4812402b2025-02-09T05:01:32ZengElsevierFood Chemistry: Molecular Sciences2666-56622025-06-0110100243Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goatsHancai Jiang0Xiaoxian Xu1Shuwan Wang2Xinhui Song3Ling Li4Qingyou Liu5Kuiqing Cui6Deshun Shi7Jian Wang8Hui Li9Jieping Huang10Zhipeng Li11Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China,Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China,Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China,Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China,Guangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China.Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, 528225, Foshan, China.Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, 528225, Foshan, China.Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China,Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China,Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China,Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China,; Corresponding authors.Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China,; Corresponding authors.Gene expression and post-transcriptional regulation are key mechanisms affecting lactation performance in dairy animals. However, the difficulty of obtaining mammary gland tissue samples from lactating animals has significantly impeded lactation research. Milk fat globules may be a non-invasive way to obtain mammary transcripts. Here, we aimed to reveal the universal rule of the transcript profiles of the milk fat globules and mammary glands from buffaloes and goats by RNA-sequencing analysis. Results showed that, in buffalo, 97 % of mRNAs were expressed in both milk fat globules and mammary glands, with 45 % showing differential expression. Among 6086 lncRNAs and 7010 miRNAs identified, 35 % and 50 % were differentially expressed, respectively. Of 11,631 circRNAs, only 618 showed significant differences. In goat, more than 99 % of mRNAs and 87 % of ncRNAs (including lncRNAs, circRNAs, and miRNAs) were expressed in both milk fat globules and mammary glands, and over 91 % of mRNAs, 96 % of lncRNAs, 98 % of circRNAs, and 86 % of miRNAs showed no significant differences, suggests that the transcripts in milk fat globules exactly reflect that in the mammary gland. This study suggests that milk fat globules are an effective candidate for non-invasive acquisition of mammary gland transcripts, but their applicability needs further study.http://www.sciencedirect.com/science/article/pii/S2666566225000048LactationTranscript profilesNon-invasive acquisitionncRNAs |
| spellingShingle | Hancai Jiang Xiaoxian Xu Shuwan Wang Xinhui Song Ling Li Qingyou Liu Kuiqing Cui Deshun Shi Jian Wang Hui Li Jieping Huang Zhipeng Li Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats Food Chemistry: Molecular Sciences Lactation Transcript profiles Non-invasive acquisition ncRNAs |
| title | Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats |
| title_full | Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats |
| title_fullStr | Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats |
| title_full_unstemmed | Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats |
| title_short | Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats |
| title_sort | characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats |
| topic | Lactation Transcript profiles Non-invasive acquisition ncRNAs |
| url | http://www.sciencedirect.com/science/article/pii/S2666566225000048 |
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