Spatially controlled multicellular differentiation of stem cells using triple factor-releasing metal–organic framework-coated nanoline arrays
Abstract Improved in vitro models are needed for regenerative therapy and drug screening. Here, we report on functionally aligned nanoparticle-trapped nanopattern arrays for spatially controlled, precise mesenchymal stem cell differentiation on a single substrate. The arrays comprise nanohole and na...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56373-0 |
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| _version_ | 1823861797164154880 |
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| author | Yeon-Woo Cho Min-Ji Kang Joon-Ha Park Yun-Sik Eom Tae-Hyung Kim |
| author_facet | Yeon-Woo Cho Min-Ji Kang Joon-Ha Park Yun-Sik Eom Tae-Hyung Kim |
| author_sort | Yeon-Woo Cho |
| collection | DOAJ |
| description | Abstract Improved in vitro models are needed for regenerative therapy and drug screening. Here, we report on functionally aligned nanoparticle-trapped nanopattern arrays for spatially controlled, precise mesenchymal stem cell differentiation on a single substrate. The arrays comprise nanohole and nanoline arrays fabricated through interference lithography and selectively capture of UiO-67 metal–organic frameworks on nanoline arrays with a 99.8% efficiency using an optimised asymmetric spin-coating method. The UiO-67 metal–organic frameworks contain three osteogenic differentiation factors for sustained release over four weeks. The combination of differentiation factors and patterned array allows for generation of adipocytes, osteoblasts, and adipocyte–osteoblast mixtures on nanohole arrays, nanoline arrays, and at the nanohole–nanoline interface, respectively, with mature osteoblasts exhibiting higher marker expression and mineralisation. The sustained release patterned array holds potential for constructing advanced therapeutic and disease state in vitro cellular models. |
| format | Article |
| id | doaj-art-45863adb71a445458742ca9a3d181891 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-45863adb71a445458742ca9a3d1818912025-02-09T12:45:51ZengNature PortfolioNature Communications2041-17232025-02-0116111210.1038/s41467-025-56373-0Spatially controlled multicellular differentiation of stem cells using triple factor-releasing metal–organic framework-coated nanoline arraysYeon-Woo Cho0Min-Ji Kang1Joon-Ha Park2Yun-Sik Eom3Tae-Hyung Kim4Department of Intelligent Precision Healthcare Convergence, Institute for Cross-disciplinary Studies (ICS), Sungkyunkwan University (SKKU)Department of Intelligent Precision Healthcare Convergence, Institute for Cross-disciplinary Studies (ICS), Sungkyunkwan University (SKKU)Department of Intelligent Precision Healthcare Convergence, Institute for Cross-disciplinary Studies (ICS), Sungkyunkwan University (SKKU)Department of Intelligent Precision Healthcare Convergence, Institute for Cross-disciplinary Studies (ICS), Sungkyunkwan University (SKKU)Department of Intelligent Precision Healthcare Convergence, Institute for Cross-disciplinary Studies (ICS), Sungkyunkwan University (SKKU)Abstract Improved in vitro models are needed for regenerative therapy and drug screening. Here, we report on functionally aligned nanoparticle-trapped nanopattern arrays for spatially controlled, precise mesenchymal stem cell differentiation on a single substrate. The arrays comprise nanohole and nanoline arrays fabricated through interference lithography and selectively capture of UiO-67 metal–organic frameworks on nanoline arrays with a 99.8% efficiency using an optimised asymmetric spin-coating method. The UiO-67 metal–organic frameworks contain three osteogenic differentiation factors for sustained release over four weeks. The combination of differentiation factors and patterned array allows for generation of adipocytes, osteoblasts, and adipocyte–osteoblast mixtures on nanohole arrays, nanoline arrays, and at the nanohole–nanoline interface, respectively, with mature osteoblasts exhibiting higher marker expression and mineralisation. The sustained release patterned array holds potential for constructing advanced therapeutic and disease state in vitro cellular models.https://doi.org/10.1038/s41467-025-56373-0 |
| spellingShingle | Yeon-Woo Cho Min-Ji Kang Joon-Ha Park Yun-Sik Eom Tae-Hyung Kim Spatially controlled multicellular differentiation of stem cells using triple factor-releasing metal–organic framework-coated nanoline arrays Nature Communications |
| title | Spatially controlled multicellular differentiation of stem cells using triple factor-releasing metal–organic framework-coated nanoline arrays |
| title_full | Spatially controlled multicellular differentiation of stem cells using triple factor-releasing metal–organic framework-coated nanoline arrays |
| title_fullStr | Spatially controlled multicellular differentiation of stem cells using triple factor-releasing metal–organic framework-coated nanoline arrays |
| title_full_unstemmed | Spatially controlled multicellular differentiation of stem cells using triple factor-releasing metal–organic framework-coated nanoline arrays |
| title_short | Spatially controlled multicellular differentiation of stem cells using triple factor-releasing metal–organic framework-coated nanoline arrays |
| title_sort | spatially controlled multicellular differentiation of stem cells using triple factor releasing metal organic framework coated nanoline arrays |
| url | https://doi.org/10.1038/s41467-025-56373-0 |
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