Metabolic changes before and after weaning in Dezhou donkey foals in relation to gut microbiota
Weaning is undoubtedly one of the most crucial stages in the growth and development of all mammalian animals, including donkey foals. Weaning is a dynamic and coordinated process of the body, which is closely associated with the health, nutrition, and metabolism of the host. Many studies have shown...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1306039/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825208812472107008 |
|---|---|
| author | Qiwen Yang Qiwen Yang Haibing Liu Halima Jafari Bing Liu Zhaofei Wang Jiangtian Su Fuwen Wang Ge Yang Minhao Sun Jie Cheng Boying Dong Min Li Mingjian Gen Jie Yu |
| author_facet | Qiwen Yang Qiwen Yang Haibing Liu Halima Jafari Bing Liu Zhaofei Wang Jiangtian Su Fuwen Wang Ge Yang Minhao Sun Jie Cheng Boying Dong Min Li Mingjian Gen Jie Yu |
| author_sort | Qiwen Yang |
| collection | DOAJ |
| description | Weaning is undoubtedly one of the most crucial stages in the growth and development of all mammalian animals, including donkey foals. Weaning is a dynamic and coordinated process of the body, which is closely associated with the health, nutrition, and metabolism of the host. Many studies have shown that the intestinal microbiota and serum metabolites of mammals exhibit different changes during lactation, weaning, and postweaning. However, the alterations in serum metabolites in donkey foals before and postweaning and the correlation between serum metabolites and intestinal microbiota are largely unknown. This study is based on the fecal 16S rRNA and serum metabolomes of Dezhou donkey foals. In total, 10 samples (fecal and serum) were collected during the following three stages: before weaning (F.M.1), during weaning (F.M.3), and postweaning (F.M.6). To study the alterations in intestinal microflora, serum metabolites, and their correlation before and postweaning. We found that with the growth and weaning progress of donkey foals, the intestinal microbiota of donkey foals underwent obvious changes, and the diversity of fecal bacteria increased (Chao1 and Shannon indexes). The main intestinal microbial flora of donkey foals include Bacteroides and Firmicutes. We found many microbiota that are associated with immunity and digestion in the postweaning group, such as Verrucomicrobiales, Clostridia, Oscillospiraceae, Akkermansia, and Rikenellaceae, which can be considered microbial markers for the transition from liquid milk to solid pellet feed. Clostridia and Oscillospiraceae can produce organic acids, including butyric acid and acetic acid, which are crucial for regulating the intestinal microecological balance of donkeys. Furthermore, the metabolome showed that the serum metabolites enriched before and postweaning were mainly related to arachidonic acid metabolism and riboflavin metabolism. Riboflavin was associated with the development of the small intestine and affected the absorption of the small intestine. We also found that the changes in the gut microbiome of the foals were significantly correlated with changes in serum metabolites, including lysophosphatidylcholine (LPC; 12,0) and positively correlated with Lachnoclostridium and Roseburia. To summarize, this study provides theoretical data for the changes in the intestinal microbiome and serum metabolism during the entire weaning period of donkey foals. |
| format | Article |
| id | doaj-art-473922449c7f43288b1b2816090568d5 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-473922449c7f43288b1b2816090568d52025-02-06T20:57:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-01-011410.3389/fmicb.2023.13060391306039Metabolic changes before and after weaning in Dezhou donkey foals in relation to gut microbiotaQiwen Yang0Qiwen Yang1Haibing Liu2Halima Jafari3Bing Liu4Zhaofei Wang5Jiangtian Su6Fuwen Wang7Ge Yang8Minhao Sun9Jie Cheng10Boying Dong11Min Li12Mingjian Gen13Jie Yu14National Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., Dong'e County, Shandong, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Xianyang, Shaanxi, ChinaNational Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., Dong'e County, Shandong, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Xianyang, Shaanxi, ChinaNational Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., Dong'e County, Shandong, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Xianyang, Shaanxi, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Xianyang, Shaanxi, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Xianyang, Shaanxi, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Xianyang, Shaanxi, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Xianyang, Shaanxi, ChinaNational Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., Dong'e County, Shandong, ChinaNational Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., Dong'e County, Shandong, ChinaNational Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., Dong'e County, Shandong, ChinaNational Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., Dong'e County, Shandong, ChinaNational Engineering Research Center for Gelatin-Based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., Dong'e County, Shandong, ChinaWeaning is undoubtedly one of the most crucial stages in the growth and development of all mammalian animals, including donkey foals. Weaning is a dynamic and coordinated process of the body, which is closely associated with the health, nutrition, and metabolism of the host. Many studies have shown that the intestinal microbiota and serum metabolites of mammals exhibit different changes during lactation, weaning, and postweaning. However, the alterations in serum metabolites in donkey foals before and postweaning and the correlation between serum metabolites and intestinal microbiota are largely unknown. This study is based on the fecal 16S rRNA and serum metabolomes of Dezhou donkey foals. In total, 10 samples (fecal and serum) were collected during the following three stages: before weaning (F.M.1), during weaning (F.M.3), and postweaning (F.M.6). To study the alterations in intestinal microflora, serum metabolites, and their correlation before and postweaning. We found that with the growth and weaning progress of donkey foals, the intestinal microbiota of donkey foals underwent obvious changes, and the diversity of fecal bacteria increased (Chao1 and Shannon indexes). The main intestinal microbial flora of donkey foals include Bacteroides and Firmicutes. We found many microbiota that are associated with immunity and digestion in the postweaning group, such as Verrucomicrobiales, Clostridia, Oscillospiraceae, Akkermansia, and Rikenellaceae, which can be considered microbial markers for the transition from liquid milk to solid pellet feed. Clostridia and Oscillospiraceae can produce organic acids, including butyric acid and acetic acid, which are crucial for regulating the intestinal microecological balance of donkeys. Furthermore, the metabolome showed that the serum metabolites enriched before and postweaning were mainly related to arachidonic acid metabolism and riboflavin metabolism. Riboflavin was associated with the development of the small intestine and affected the absorption of the small intestine. We also found that the changes in the gut microbiome of the foals were significantly correlated with changes in serum metabolites, including lysophosphatidylcholine (LPC; 12,0) and positively correlated with Lachnoclostridium and Roseburia. To summarize, this study provides theoretical data for the changes in the intestinal microbiome and serum metabolism during the entire weaning period of donkey foals.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1306039/fulldonkey foalweaninggut microbesserummetabolome |
| spellingShingle | Qiwen Yang Qiwen Yang Haibing Liu Halima Jafari Bing Liu Zhaofei Wang Jiangtian Su Fuwen Wang Ge Yang Minhao Sun Jie Cheng Boying Dong Min Li Mingjian Gen Jie Yu Metabolic changes before and after weaning in Dezhou donkey foals in relation to gut microbiota Frontiers in Microbiology donkey foal weaning gut microbes serum metabolome |
| title | Metabolic changes before and after weaning in Dezhou donkey foals in relation to gut microbiota |
| title_full | Metabolic changes before and after weaning in Dezhou donkey foals in relation to gut microbiota |
| title_fullStr | Metabolic changes before and after weaning in Dezhou donkey foals in relation to gut microbiota |
| title_full_unstemmed | Metabolic changes before and after weaning in Dezhou donkey foals in relation to gut microbiota |
| title_short | Metabolic changes before and after weaning in Dezhou donkey foals in relation to gut microbiota |
| title_sort | metabolic changes before and after weaning in dezhou donkey foals in relation to gut microbiota |
| topic | donkey foal weaning gut microbes serum metabolome |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1306039/full |
| work_keys_str_mv | AT qiwenyang metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT qiwenyang metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT haibingliu metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT halimajafari metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT bingliu metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT zhaofeiwang metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT jiangtiansu metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT fuwenwang metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT geyang metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT minhaosun metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT jiecheng metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT boyingdong metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT minli metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT mingjiangen metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota AT jieyu metabolicchangesbeforeandafterweaningindezhoudonkeyfoalsinrelationtogutmicrobiota |