Investigation of new exopolysaccharides produced by strains of donkey milk
Background and Objectives: It has been shown that strains of the genus Enterococcus isolated from donkey milk from different regions of the Republic of Armenia have antimicrobial activity, synthesize different polysaccharides and produce disaccharide polymers (glucose and galactose). The quantitati...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2025-02-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/5124 |
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| author | Arevik Israyelyan Tsovinar Balabekyan Lara Aleksanyan Inesa Sahakyan Anna Gasparyan Flora Тkhruni |
| author_facet | Arevik Israyelyan Tsovinar Balabekyan Lara Aleksanyan Inesa Sahakyan Anna Gasparyan Flora Тkhruni |
| author_sort | Arevik Israyelyan |
| collection | DOAJ |
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Background and Objectives: It has been shown that strains of the genus Enterococcus isolated from donkey milk from different regions of the Republic of Armenia have antimicrobial activity, synthesize different polysaccharides and produce disaccharide polymers (glucose and galactose). The quantitative synthesis of polysaccharides (8-15%) depends on the composition of the nutrient medium, temperature and growing time.
Materials and Methods: Species identification of LAB strains was confirmed by 16S rDNA gene sequencing method using universal primers LAB strains. The methods used for extraction, purification and detection of exopolysaccharides are based on the method of Sørensen et al. (2022). The antibacterial activity of EPS was investigated by agar diffusion assay. Determination of the immunostimulating property was carried out using the ELISA method.
Results: The antimicrobial activity of the polysaccharide and antimicrobial protein-like fractions of the genus Enterococcus strains depends on its concentration, time of interaction with the test culture, and the species of the pathogenic bacteria.
Conclusion: The obtained results were shown that strains isolated from fermented donkey milk that are capable of synthesizing two substances of different nature with high antimicrobial properties during the growth process are promising for further research and application for their use as probiotics and biopreparations in pharmaceuticals.
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| format | Article |
| id | doaj-art-475637e18fcd4afdb04460c9b15d0226 |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-475637e18fcd4afdb04460c9b15d02262025-02-09T09:01:26ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472025-02-01171Investigation of new exopolysaccharides produced by strains of donkey milkArevik Israyelyan0Tsovinar Balabekyan1Lara Aleksanyan2Inesa Sahakyan3Anna Gasparyan4Flora Тkhruni5Scientific and Production Center “Armbiotechnology” NSPO, National Academy of Science, Probiotics Production Sector, Yerevan, Republic of ArmeniaScientific and Production Center “Armbiotechnology” NSPO, National Academy of Science, Probiotics Production Sector, Yerevan, Republic of ArmeniaScientific and Production Center “Armbiotechnology” NSPO, National Academy of Science, Probiotics Production Sector, Yerevan, Republic of ArmeniaDepartment Histochemistry and Functional Morphology, Buniatyan Institute of Biochemistry National Academy of Science, Yerevan, Republic of ArmeniaScientific and Production Center “Armbiotechnology” NSPO, National Academy of Science, Probiotics Production Sector, Yerevan, Republic of ArmeniaScientific and Production Center “Armbiotechnology” NSPO, National Academy of Science, Probiotics Production Sector, Yerevan, Republic of Armenia Background and Objectives: It has been shown that strains of the genus Enterococcus isolated from donkey milk from different regions of the Republic of Armenia have antimicrobial activity, synthesize different polysaccharides and produce disaccharide polymers (glucose and galactose). The quantitative synthesis of polysaccharides (8-15%) depends on the composition of the nutrient medium, temperature and growing time. Materials and Methods: Species identification of LAB strains was confirmed by 16S rDNA gene sequencing method using universal primers LAB strains. The methods used for extraction, purification and detection of exopolysaccharides are based on the method of Sørensen et al. (2022). The antibacterial activity of EPS was investigated by agar diffusion assay. Determination of the immunostimulating property was carried out using the ELISA method. Results: The antimicrobial activity of the polysaccharide and antimicrobial protein-like fractions of the genus Enterococcus strains depends on its concentration, time of interaction with the test culture, and the species of the pathogenic bacteria. Conclusion: The obtained results were shown that strains isolated from fermented donkey milk that are capable of synthesizing two substances of different nature with high antimicrobial properties during the growth process are promising for further research and application for their use as probiotics and biopreparations in pharmaceuticals. https://ijm.tums.ac.ir/index.php/ijm/article/view/5124Exopolysaccharides;Protein-like fractions;Interaction;Donkey milk;Antimicrobial activity |
| spellingShingle | Arevik Israyelyan Tsovinar Balabekyan Lara Aleksanyan Inesa Sahakyan Anna Gasparyan Flora Тkhruni Investigation of new exopolysaccharides produced by strains of donkey milk Iranian Journal of Microbiology Exopolysaccharides; Protein-like fractions; Interaction; Donkey milk; Antimicrobial activity |
| title | Investigation of new exopolysaccharides produced by strains of donkey milk |
| title_full | Investigation of new exopolysaccharides produced by strains of donkey milk |
| title_fullStr | Investigation of new exopolysaccharides produced by strains of donkey milk |
| title_full_unstemmed | Investigation of new exopolysaccharides produced by strains of donkey milk |
| title_short | Investigation of new exopolysaccharides produced by strains of donkey milk |
| title_sort | investigation of new exopolysaccharides produced by strains of donkey milk |
| topic | Exopolysaccharides; Protein-like fractions; Interaction; Donkey milk; Antimicrobial activity |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/5124 |
| work_keys_str_mv | AT arevikisrayelyan investigationofnewexopolysaccharidesproducedbystrainsofdonkeymilk AT tsovinarbalabekyan investigationofnewexopolysaccharidesproducedbystrainsofdonkeymilk AT laraaleksanyan investigationofnewexopolysaccharidesproducedbystrainsofdonkeymilk AT inesasahakyan investigationofnewexopolysaccharidesproducedbystrainsofdonkeymilk AT annagasparyan investigationofnewexopolysaccharidesproducedbystrainsofdonkeymilk AT floratkhruni investigationofnewexopolysaccharidesproducedbystrainsofdonkeymilk |