Synthesis and Antioxidant Activity of Selenized Apigenin
Selenized apigenin (Se-AP) synthesized using sodium selenite employed as a highly reactive selenide reagent and the flavonoid apigenin (AP) as a substrate was investigated in this paper. Followed by a series of analytical techniques were used to characterize structure of Se-AP and extrapolate the po...
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The editorial department of Science and Technology of Food Industry
2025-02-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030269 |
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| author | Yawen GAO Guopeng WU Bo HAN Rongwu WANG Fangming LOU |
| author_facet | Yawen GAO Guopeng WU Bo HAN Rongwu WANG Fangming LOU |
| author_sort | Yawen GAO |
| collection | DOAJ |
| description | Selenized apigenin (Se-AP) synthesized using sodium selenite employed as a highly reactive selenide reagent and the flavonoid apigenin (AP) as a substrate was investigated in this paper. Followed by a series of analytical techniques were used to characterize structure of Se-AP and extrapolate the possible coordination structures in Se-AP. Se content of Se-AP was measured from the fluorescent spectrophotometry to be about 1.91%±0.11%. Based on the results of structural characterization analysis, there could be concluded that new Se-O and C-O-Se bonds were formed without altering the main structure of the flavonoid backbone in selenized apigenin. The Se elements were evenly distributed in Se-AP, and one possible coordination structure of Se-AP was inferred. In addition, the antioxidant activity of AP and Se-AP was investigated by designing ABTS+, DPPH, and OH radical scavenging experiments. The in vitro antioxidant results suggested that Se-AP exhibited a clearance rate of 93.82%±0.63% for ABTS+ radicals at a concentration of 400 mg/L, 39.53%±0.67% for DPPH radicals and 96.54%±0.77% for OH radicals at a concentration of 800 mg/L and the ability of scavenging DPPH free radicals was slightly enhanced, while the scavenging ability of ABTS+ and OH radicals was significantly increased (IC50 was 53.52±1.85 mg/L and 11.33±0.64 mg/L), and the scavenging ability was comparable to that of VC at 800 mg/L at the same concentration for Se-AP. Therefore, the present data indicated that selenylation modification of AP could increase the antioxidant activity and enhance the biological activity, which could offer the possibility of the development and utilization of selenized flavonoid-related foods and drugs. |
| format | Article |
| id | doaj-art-49372b9c7b9d489d8c8f64b6652d1b47 |
| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-49372b9c7b9d489d8c8f64b6652d1b472025-02-08T08:06:20ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-02-0146410911610.13386/j.issn1002-0306.20240302692024030269-4Synthesis and Antioxidant Activity of Selenized ApigeninYawen GAO0Guopeng WU1Bo HAN2Rongwu WANG3Fangming LOU4College of Chemistry and Environmental Engineering, Hubei Minzu University, Enshi 445000, ChinaCollege of Chemistry and Environmental Engineering, Hubei Minzu University, Enshi 445000, ChinaCollege of Chemistry and Environmental Engineering, Hubei Minzu University, Enshi 445000, ChinaCollege of Chemistry and Environmental Engineering, Hubei Minzu University, Enshi 445000, ChinaCollege of Chemistry and Environmental Engineering, Hubei Minzu University, Enshi 445000, ChinaSelenized apigenin (Se-AP) synthesized using sodium selenite employed as a highly reactive selenide reagent and the flavonoid apigenin (AP) as a substrate was investigated in this paper. Followed by a series of analytical techniques were used to characterize structure of Se-AP and extrapolate the possible coordination structures in Se-AP. Se content of Se-AP was measured from the fluorescent spectrophotometry to be about 1.91%±0.11%. Based on the results of structural characterization analysis, there could be concluded that new Se-O and C-O-Se bonds were formed without altering the main structure of the flavonoid backbone in selenized apigenin. The Se elements were evenly distributed in Se-AP, and one possible coordination structure of Se-AP was inferred. In addition, the antioxidant activity of AP and Se-AP was investigated by designing ABTS+, DPPH, and OH radical scavenging experiments. The in vitro antioxidant results suggested that Se-AP exhibited a clearance rate of 93.82%±0.63% for ABTS+ radicals at a concentration of 400 mg/L, 39.53%±0.67% for DPPH radicals and 96.54%±0.77% for OH radicals at a concentration of 800 mg/L and the ability of scavenging DPPH free radicals was slightly enhanced, while the scavenging ability of ABTS+ and OH radicals was significantly increased (IC50 was 53.52±1.85 mg/L and 11.33±0.64 mg/L), and the scavenging ability was comparable to that of VC at 800 mg/L at the same concentration for Se-AP. Therefore, the present data indicated that selenylation modification of AP could increase the antioxidant activity and enhance the biological activity, which could offer the possibility of the development and utilization of selenized flavonoid-related foods and drugs.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030269flavonoidsapigeninselenated apigenincoordination structureantioxidant activity |
| spellingShingle | Yawen GAO Guopeng WU Bo HAN Rongwu WANG Fangming LOU Synthesis and Antioxidant Activity of Selenized Apigenin Shipin gongye ke-ji flavonoids apigenin selenated apigenin coordination structure antioxidant activity |
| title | Synthesis and Antioxidant Activity of Selenized Apigenin |
| title_full | Synthesis and Antioxidant Activity of Selenized Apigenin |
| title_fullStr | Synthesis and Antioxidant Activity of Selenized Apigenin |
| title_full_unstemmed | Synthesis and Antioxidant Activity of Selenized Apigenin |
| title_short | Synthesis and Antioxidant Activity of Selenized Apigenin |
| title_sort | synthesis and antioxidant activity of selenized apigenin |
| topic | flavonoids apigenin selenated apigenin coordination structure antioxidant activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030269 |
| work_keys_str_mv | AT yawengao synthesisandantioxidantactivityofselenizedapigenin AT guopengwu synthesisandantioxidantactivityofselenizedapigenin AT bohan synthesisandantioxidantactivityofselenizedapigenin AT rongwuwang synthesisandantioxidantactivityofselenizedapigenin AT fangminglou synthesisandantioxidantactivityofselenizedapigenin |