Transcriptome atlases of rat brain regions and their adaptation to diabetes resolution following gastrectomy in the Goto-Kakizaki rat
Abstract Brain regions drive multiple physiological functions through specific gene expression patterns that adapt to environmental influences, drug treatments and disease conditions. To generate a detailed atlas of the brain transcriptome in the context of diabetes, we carried out RNA sequencing in...
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BMC
2025-02-01
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| Series: | Molecular Brain |
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| Online Access: | https://doi.org/10.1186/s13041-025-01176-z |
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| author | François Brial Aurélie Le Lay Claude Rouch Edouard Henrion Mathieu Bourgey Guillaume Bourque Mark Lathrop Christophe Magnan Dominique Gauguier |
| author_facet | François Brial Aurélie Le Lay Claude Rouch Edouard Henrion Mathieu Bourgey Guillaume Bourque Mark Lathrop Christophe Magnan Dominique Gauguier |
| author_sort | François Brial |
| collection | DOAJ |
| description | Abstract Brain regions drive multiple physiological functions through specific gene expression patterns that adapt to environmental influences, drug treatments and disease conditions. To generate a detailed atlas of the brain transcriptome in the context of diabetes, we carried out RNA sequencing in hypothalamus, hippocampus, brainstem and striatum of the Goto-Kakizaki (GK) rat model of spontaneous type 2 diabetes, which was applied to identify gene transcription adaptation to improved glycemic control following vertical sleeve gastrectomy (VSG) in the GK. Over 19,000 distinct transcripts were detected in the rat brain, including 2794 which were consistently expressed in the four brain regions. Region-specific gene expression was identified in hypothalamus (n = 477), hippocampus (n = 468), brainstem (n = 1173) and striatum (n = 791), resulting in differential regulation of biological processes between regions. Differentially expressed genes between VSG and sham operated rats were only found in the hypothalamus and were predominantly involved in the regulation of endothelium and extracellular matrix. These results provide a detailed atlas of regional gene expression in the diabetic rat brain and suggest that the long term effects of gastrectomy-promoted diabetes remission involve functional changes in the hypothalamus endothelium. |
| format | Article |
| id | doaj-art-4b055810960d4f9f9aa4e4e909e82330 |
| institution | Kabale University |
| issn | 1756-6606 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj-art-4b055810960d4f9f9aa4e4e909e823302025-02-09T13:00:15ZengBMCMolecular Brain1756-66062025-02-0118111510.1186/s13041-025-01176-zTranscriptome atlases of rat brain regions and their adaptation to diabetes resolution following gastrectomy in the Goto-Kakizaki ratFrançois Brial0Aurélie Le Lay1Claude Rouch2Edouard Henrion3Mathieu Bourgey4Guillaume Bourque5Mark Lathrop6Christophe Magnan7Dominique Gauguier8Université Paris Cité, INSERM U1132 Biologie de l’os et du Cartilage (BIOSCAR)Université Paris Cité, Functional and Adaptive Biology, UMR 8251, CNRSUniversité Paris Cité, Functional and Adaptive Biology, UMR 8251, CNRSVictor Phillip Dahdaleh Institute of Genomic Medicine at McGill UniversityVictor Phillip Dahdaleh Institute of Genomic Medicine at McGill UniversityVictor Phillip Dahdaleh Institute of Genomic Medicine at McGill UniversityVictor Phillip Dahdaleh Institute of Genomic Medicine at McGill UniversityUniversité Paris Cité, Functional and Adaptive Biology, UMR 8251, CNRSUniversité Paris Cité, Functional and Adaptive Biology, UMR 8251, CNRSAbstract Brain regions drive multiple physiological functions through specific gene expression patterns that adapt to environmental influences, drug treatments and disease conditions. To generate a detailed atlas of the brain transcriptome in the context of diabetes, we carried out RNA sequencing in hypothalamus, hippocampus, brainstem and striatum of the Goto-Kakizaki (GK) rat model of spontaneous type 2 diabetes, which was applied to identify gene transcription adaptation to improved glycemic control following vertical sleeve gastrectomy (VSG) in the GK. Over 19,000 distinct transcripts were detected in the rat brain, including 2794 which were consistently expressed in the four brain regions. Region-specific gene expression was identified in hypothalamus (n = 477), hippocampus (n = 468), brainstem (n = 1173) and striatum (n = 791), resulting in differential regulation of biological processes between regions. Differentially expressed genes between VSG and sham operated rats were only found in the hypothalamus and were predominantly involved in the regulation of endothelium and extracellular matrix. These results provide a detailed atlas of regional gene expression in the diabetic rat brain and suggest that the long term effects of gastrectomy-promoted diabetes remission involve functional changes in the hypothalamus endothelium.https://doi.org/10.1186/s13041-025-01176-zBariatric surgeryVertical sleeve gastrectomyRNA sequencing |
| spellingShingle | François Brial Aurélie Le Lay Claude Rouch Edouard Henrion Mathieu Bourgey Guillaume Bourque Mark Lathrop Christophe Magnan Dominique Gauguier Transcriptome atlases of rat brain regions and their adaptation to diabetes resolution following gastrectomy in the Goto-Kakizaki rat Molecular Brain Bariatric surgery Vertical sleeve gastrectomy RNA sequencing |
| title | Transcriptome atlases of rat brain regions and their adaptation to diabetes resolution following gastrectomy in the Goto-Kakizaki rat |
| title_full | Transcriptome atlases of rat brain regions and their adaptation to diabetes resolution following gastrectomy in the Goto-Kakizaki rat |
| title_fullStr | Transcriptome atlases of rat brain regions and their adaptation to diabetes resolution following gastrectomy in the Goto-Kakizaki rat |
| title_full_unstemmed | Transcriptome atlases of rat brain regions and their adaptation to diabetes resolution following gastrectomy in the Goto-Kakizaki rat |
| title_short | Transcriptome atlases of rat brain regions and their adaptation to diabetes resolution following gastrectomy in the Goto-Kakizaki rat |
| title_sort | transcriptome atlases of rat brain regions and their adaptation to diabetes resolution following gastrectomy in the goto kakizaki rat |
| topic | Bariatric surgery Vertical sleeve gastrectomy RNA sequencing |
| url | https://doi.org/10.1186/s13041-025-01176-z |
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