Screening of soybean antifungal isoflavones based on targeted metabolomics analysis
Soybean (Glycine max) is a key crop rich in bioactive compounds, particularly isoflavones, which play a significant role in plant defense against biotic stress like fungal infections. In this study, nine soybean varieties with varying susceptibility to mildew were evaluated. Isoflavone profiles in s...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525000410 |
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| author | Nishbah Mughal Xiaowen Zhang Noman Shoaib Juncai Deng Jinya Guo Jing Zhang Wenyu Yang Jiang Liu |
| author_facet | Nishbah Mughal Xiaowen Zhang Noman Shoaib Juncai Deng Jinya Guo Jing Zhang Wenyu Yang Jiang Liu |
| author_sort | Nishbah Mughal |
| collection | DOAJ |
| description | Soybean (Glycine max) is a key crop rich in bioactive compounds, particularly isoflavones, which play a significant role in plant defense against biotic stress like fungal infections. In this study, nine soybean varieties with varying susceptibility to mildew were evaluated. Isoflavone profiles in seeds and pods were analyzed using high-performance liquid chromatography-mass spectrometry (HPLC-MS), and metabolomics analysis via orthogonal signal correction partial least squares discriminant analysis (OSC-PLS-DA) identified differences between mildew-affected and unaffected samples. Results showed organ-specific changes, with isoflavone aglycones increasing in seeds, while malonylglucosides (M-type) varied in pods. β-Glucoside (G-type) and M-type isoflavones were identified as differential metabolites. Antifungal assays revealed that genistin, among six isoflavone glycosides and aglycones tested, had the strongest inhibitory effects on Aspergillus flavus. Additionally, the identification of G-type and M-type isoflavone glycosides underscores the necessity for further investigation into the roles these metabolites play in the overall antifungal activity observed. |
| format | Article |
| id | doaj-art-4d7fc8687d03423bbb9a3d9b06ebf792 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-4d7fc8687d03423bbb9a3d9b06ebf7922025-02-12T05:32:30ZengElsevierFood Chemistry: X2590-15752025-01-0125102195Screening of soybean antifungal isoflavones based on targeted metabolomics analysisNishbah Mughal0Xiaowen Zhang1Noman Shoaib2Juncai Deng3Jinya Guo4Jing Zhang5Wenyu Yang6Jiang Liu7College of Life Science, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Engineering Research Center for Crop Strip Intercropping System, Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, ChinaSichuan Engineering Research Center for Crop Strip Intercropping System, Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, ChinaCAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaCollege of Life Science, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Engineering Research Center for Crop Strip Intercropping System, Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Life Science, Sichuan Agricultural University, Ya'an 625014, ChinaSichuan Engineering Research Center for Crop Strip Intercropping System, Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, ChinaSichuan Engineering Research Center for Crop Strip Intercropping System, Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Life Science, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Engineering Research Center for Crop Strip Intercropping System, Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture and Rural Affairs, Sichuan Agricultural University, Chengdu 611130, China; Corresponding author at: College of Life Science, Sichuan Agricultural University, Ya'an 625014, China.Soybean (Glycine max) is a key crop rich in bioactive compounds, particularly isoflavones, which play a significant role in plant defense against biotic stress like fungal infections. In this study, nine soybean varieties with varying susceptibility to mildew were evaluated. Isoflavone profiles in seeds and pods were analyzed using high-performance liquid chromatography-mass spectrometry (HPLC-MS), and metabolomics analysis via orthogonal signal correction partial least squares discriminant analysis (OSC-PLS-DA) identified differences between mildew-affected and unaffected samples. Results showed organ-specific changes, with isoflavone aglycones increasing in seeds, while malonylglucosides (M-type) varied in pods. β-Glucoside (G-type) and M-type isoflavones were identified as differential metabolites. Antifungal assays revealed that genistin, among six isoflavone glycosides and aglycones tested, had the strongest inhibitory effects on Aspergillus flavus. Additionally, the identification of G-type and M-type isoflavone glycosides underscores the necessity for further investigation into the roles these metabolites play in the overall antifungal activity observed.http://www.sciencedirect.com/science/article/pii/S2590157525000410SoybeanTargeted metabolomicsFungal infectionField midlewIsoflavone |
| spellingShingle | Nishbah Mughal Xiaowen Zhang Noman Shoaib Juncai Deng Jinya Guo Jing Zhang Wenyu Yang Jiang Liu Screening of soybean antifungal isoflavones based on targeted metabolomics analysis Food Chemistry: X Soybean Targeted metabolomics Fungal infection Field midlew Isoflavone |
| title | Screening of soybean antifungal isoflavones based on targeted metabolomics analysis |
| title_full | Screening of soybean antifungal isoflavones based on targeted metabolomics analysis |
| title_fullStr | Screening of soybean antifungal isoflavones based on targeted metabolomics analysis |
| title_full_unstemmed | Screening of soybean antifungal isoflavones based on targeted metabolomics analysis |
| title_short | Screening of soybean antifungal isoflavones based on targeted metabolomics analysis |
| title_sort | screening of soybean antifungal isoflavones based on targeted metabolomics analysis |
| topic | Soybean Targeted metabolomics Fungal infection Field midlew Isoflavone |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525000410 |
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