Growth Factor Comparison in Cortical Demineralized Bone Matrix that Demineralized Using Chloric and Acetic Acid
Background: Demineralized bone matrix (DBM) is an alternative biomaterial for which specific acid and immersion time are needed to optimize growth factor preservation. The optimal demineralization protocol for preserving growth factors in DBM remains unclear. This study investigated DBM extraction m...
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Universitas Airlangga
2023-04-01
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| Series: | Journal Orthopaedi and Traumatology Surabaya |
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| Online Access: | https://e-journal.unair.ac.id/JOINTS/article/view/43414 |
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| author | Mouli Edward Ferdiansyah Mahyudin Mohammad Hardian Basuki Heri Suroto Ferdiansyah Danang Perwira |
| author_facet | Mouli Edward Ferdiansyah Mahyudin Mohammad Hardian Basuki Heri Suroto Ferdiansyah Danang Perwira |
| author_sort | Mouli Edward |
| collection | DOAJ |
| description | Background: Demineralized bone matrix (DBM) is an alternative biomaterial for which specific acid and immersion time are needed to optimize growth factor preservation. The optimal demineralization protocol for preserving growth factors in DBM remains unclear. This study investigated DBM extraction methods using different acids and immersion times to maintain optimal growth factor preservation.
Methods: This in vitro experimental laboratory study used a randomized controlled post-test-only group design. We characterized the Insulin-like growth factor-1 (IGF-1), Bone morphogenetic protein-2 (BMP-2), and Transforming growth factor-β (TGF-β) content of 1 gram of New Zealand White Rabbit cortical bone immersed in 0.6 M hydrochloric acid and 0.5 M acetic acid for 3, 6, and 9 days. We then analyzed the differences in growth factor levels in each acid and performed statistical analysis.
Results: IGF-1 levels were higher in DBM demineralized with acetic acid than with hydrochloric acid. BMP-2 and TGF-β levels were higher in DBM demineralized using hydrochloric acid. The concentration of growth factors decreased over time in DBM demineralized using acetic acid. The highest growth factor level was obtained after 6 days of immersion in hydrochloric acid.
Conclusion: DBM demineralized with acetic acid yielded higher average IGF-1 levels compared to hydrochloric acid. However, BMP-2 and TGF-β levels were higher with hydrochloric acid. Growth factor levels in hydrochloric acid peaked at 6 days and then decreased. These results suggest that avoiding over-demineralization is important for maintaining growth factor levels. Further research is needed to optimize DBM processing. |
| format | Article |
| id | doaj-art-55abb21ba25047ac8fde1974b6afe705 |
| institution | Kabale University |
| issn | 2722-712X 2460-8742 |
| language | English |
| publishDate | 2023-04-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal Orthopaedi and Traumatology Surabaya |
| spelling | doaj-art-55abb21ba25047ac8fde1974b6afe7052025-02-12T07:28:05ZengUniversitas AirlanggaJournal Orthopaedi and Traumatology Surabaya2722-712X2460-87422023-04-011211910.20473/joints.v12i1.2023.1-941496Growth Factor Comparison in Cortical Demineralized Bone Matrix that Demineralized Using Chloric and Acetic AcidMouli Edward0https://orcid.org/0000-0003-2912-3541Ferdiansyah Mahyudin1https://orcid.org/0000-0001-8757-9251Mohammad Hardian Basuki2https://orcid.org/0000-0003-2845-965XHeri Suroto3https://orcid.org/0000-0002-9384-897XFerdiansyah Danang Perwira4https://orcid.org/0000-0001-9556-7015Department of Orthopedics and Traumatology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Orthopedics and Traumatology, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia; Cell and Tissue Bank-Regenerative Medicine, Dr. Soetomo General Academic Hospital, Surabaya, IndonesiaDepartment of Orthopedics and Traumatology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Orthopedics and Traumatology, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia; Cell and Tissue Bank-Regenerative Medicine, Dr. Soetomo General Academic Hospital, Surabaya, IndonesiaDepartment of Orthopedics and Traumatology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Orthopedics and Traumatology, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia; Cell and Tissue Bank-Regenerative Medicine, Dr. Soetomo General Academic Hospital, Surabaya, IndonesiaDepartment of Orthopedics and Traumatology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Orthopedics and Traumatology, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia; Cell and Tissue Bank-Regenerative Medicine, Dr. Soetomo General Academic Hospital, Surabaya, IndonesiaDepartment of Orthopedics and Traumatology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Orthopedics and Traumatology, Dr. Soetomo General Academic Hospital, Surabaya, IndonesiaBackground: Demineralized bone matrix (DBM) is an alternative biomaterial for which specific acid and immersion time are needed to optimize growth factor preservation. The optimal demineralization protocol for preserving growth factors in DBM remains unclear. This study investigated DBM extraction methods using different acids and immersion times to maintain optimal growth factor preservation. Methods: This in vitro experimental laboratory study used a randomized controlled post-test-only group design. We characterized the Insulin-like growth factor-1 (IGF-1), Bone morphogenetic protein-2 (BMP-2), and Transforming growth factor-β (TGF-β) content of 1 gram of New Zealand White Rabbit cortical bone immersed in 0.6 M hydrochloric acid and 0.5 M acetic acid for 3, 6, and 9 days. We then analyzed the differences in growth factor levels in each acid and performed statistical analysis. Results: IGF-1 levels were higher in DBM demineralized with acetic acid than with hydrochloric acid. BMP-2 and TGF-β levels were higher in DBM demineralized using hydrochloric acid. The concentration of growth factors decreased over time in DBM demineralized using acetic acid. The highest growth factor level was obtained after 6 days of immersion in hydrochloric acid. Conclusion: DBM demineralized with acetic acid yielded higher average IGF-1 levels compared to hydrochloric acid. However, BMP-2 and TGF-β levels were higher with hydrochloric acid. Growth factor levels in hydrochloric acid peaked at 6 days and then decreased. These results suggest that avoiding over-demineralization is important for maintaining growth factor levels. Further research is needed to optimize DBM processing.https://e-journal.unair.ac.id/JOINTS/article/view/43414bone morphogenetic protein-2demineralized bone matrixhuman and medicineinsulin-like growth factor-1transforming growth factor-β |
| spellingShingle | Mouli Edward Ferdiansyah Mahyudin Mohammad Hardian Basuki Heri Suroto Ferdiansyah Danang Perwira Growth Factor Comparison in Cortical Demineralized Bone Matrix that Demineralized Using Chloric and Acetic Acid Journal Orthopaedi and Traumatology Surabaya bone morphogenetic protein-2 demineralized bone matrix human and medicine insulin-like growth factor-1 transforming growth factor-β |
| title | Growth Factor Comparison in Cortical Demineralized Bone Matrix that Demineralized Using Chloric and Acetic Acid |
| title_full | Growth Factor Comparison in Cortical Demineralized Bone Matrix that Demineralized Using Chloric and Acetic Acid |
| title_fullStr | Growth Factor Comparison in Cortical Demineralized Bone Matrix that Demineralized Using Chloric and Acetic Acid |
| title_full_unstemmed | Growth Factor Comparison in Cortical Demineralized Bone Matrix that Demineralized Using Chloric and Acetic Acid |
| title_short | Growth Factor Comparison in Cortical Demineralized Bone Matrix that Demineralized Using Chloric and Acetic Acid |
| title_sort | growth factor comparison in cortical demineralized bone matrix that demineralized using chloric and acetic acid |
| topic | bone morphogenetic protein-2 demineralized bone matrix human and medicine insulin-like growth factor-1 transforming growth factor-β |
| url | https://e-journal.unair.ac.id/JOINTS/article/view/43414 |
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