Impact of Paracoccus sp. EGY7 carotenoids on triple-negative breast cancer cells: invitro study
Abstract This study investigates the effect of Paracoccus sp. EGY7 carotenoids on the triple-negative breast cancer cell line (MDA-MB-231). The bacterial strain was isolated, and its carotenoids profile was analyzed via HPLC-DAD-MS. Cytotoxicity, migration tests and the expression of BAX and BCL-2 a...
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2025-02-01
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| Online Access: | https://doi.org/10.1186/s13568-025-01825-5 |
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| author | Karim Abdelazim Ahmed Hussein Sherine N. Khattab Shaymaa Essam El Feky Nehad Noby |
| author_facet | Karim Abdelazim Ahmed Hussein Sherine N. Khattab Shaymaa Essam El Feky Nehad Noby |
| author_sort | Karim Abdelazim |
| collection | DOAJ |
| description | Abstract This study investigates the effect of Paracoccus sp. EGY7 carotenoids on the triple-negative breast cancer cell line (MDA-MB-231). The bacterial strain was isolated, and its carotenoids profile was analyzed via HPLC-DAD-MS. Cytotoxicity, migration tests and the expression of BAX and BCL-2 at the gene and protein levels were carried out to evaluate the therapeutic efficiency of the produced carotenoids. Molecular docking analysis estimated the binding affinity between zeaxanthin and BCL-2 protein. Chromatographic analysis revealed zeaxanthin as the major carotenoid (48.3%). The extract exhibited significant cytotoxicity against MDA-MB-231 cells with an IC50 of 1200 µg. It notably reduced cell migration, with wound closure percentages of 37.50% and 79.17% for the 600 µg group, while the percentages were 12.50% and 53.50% for the 1200 µg group, compared to 71.67% and 95.67% for the control at 24 and 48 h post-treatment, respectively. The extract induced apoptosis, as evidenced by significantly increased BAX/BCL-2 gene expression ratios at 600 and 1200 µg (p < 0.05). Western blotting showed increased BAX protein expression at 600 and 1200 µg compared to the control group (p < 0.001), and significantly lower BCL-2 protein expression (p = 0.000005 for 1200 µg and p = 0.0001 for 600 µg). Docking analysis indicated a strong affinity of zeaxanthin to BCL-2 (ΔG = -9.773241 kcal/mol) compared to obatoclax (ΔG = -7.419345 kcal/mol). Paracoccus sp. EGY7 carotenoids are a promising anticancer agent against MDA-MB-231 cells. They effectively promote apoptosis and prevent metastasis, crucial for disease advancement in cancer cells. |
| format | Article |
| id | doaj-art-56a5d3f633a04a44a96218ab59ca2f03 |
| institution | Kabale University |
| issn | 2191-0855 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj-art-56a5d3f633a04a44a96218ab59ca2f032025-02-09T12:55:51ZengSpringerOpenAMB Express2191-08552025-02-0115111610.1186/s13568-025-01825-5Impact of Paracoccus sp. EGY7 carotenoids on triple-negative breast cancer cells: invitro studyKarim Abdelazim0Ahmed Hussein1Sherine N. Khattab2Shaymaa Essam El Feky3Nehad Noby4Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria UniversityDepartment of Biotechnology, Institute of Graduate Studies and Research, Alexandria UniversityChemistery Department, Faculty of Science, Alexandria UniversityRadiation Sciences Department, Medical Research Institute, University of AlexandriaDepartment of Biotechnology, Institute of Graduate Studies and Research, Alexandria UniversityAbstract This study investigates the effect of Paracoccus sp. EGY7 carotenoids on the triple-negative breast cancer cell line (MDA-MB-231). The bacterial strain was isolated, and its carotenoids profile was analyzed via HPLC-DAD-MS. Cytotoxicity, migration tests and the expression of BAX and BCL-2 at the gene and protein levels were carried out to evaluate the therapeutic efficiency of the produced carotenoids. Molecular docking analysis estimated the binding affinity between zeaxanthin and BCL-2 protein. Chromatographic analysis revealed zeaxanthin as the major carotenoid (48.3%). The extract exhibited significant cytotoxicity against MDA-MB-231 cells with an IC50 of 1200 µg. It notably reduced cell migration, with wound closure percentages of 37.50% and 79.17% for the 600 µg group, while the percentages were 12.50% and 53.50% for the 1200 µg group, compared to 71.67% and 95.67% for the control at 24 and 48 h post-treatment, respectively. The extract induced apoptosis, as evidenced by significantly increased BAX/BCL-2 gene expression ratios at 600 and 1200 µg (p < 0.05). Western blotting showed increased BAX protein expression at 600 and 1200 µg compared to the control group (p < 0.001), and significantly lower BCL-2 protein expression (p = 0.000005 for 1200 µg and p = 0.0001 for 600 µg). Docking analysis indicated a strong affinity of zeaxanthin to BCL-2 (ΔG = -9.773241 kcal/mol) compared to obatoclax (ΔG = -7.419345 kcal/mol). Paracoccus sp. EGY7 carotenoids are a promising anticancer agent against MDA-MB-231 cells. They effectively promote apoptosis and prevent metastasis, crucial for disease advancement in cancer cells.https://doi.org/10.1186/s13568-025-01825-5AnticancerAntioxidantsCarotenoidsXanthophyllsParacoccus sp.Zeaxanthin. |
| spellingShingle | Karim Abdelazim Ahmed Hussein Sherine N. Khattab Shaymaa Essam El Feky Nehad Noby Impact of Paracoccus sp. EGY7 carotenoids on triple-negative breast cancer cells: invitro study AMB Express Anticancer Antioxidants Carotenoids Xanthophylls Paracoccus sp. Zeaxanthin. |
| title | Impact of Paracoccus sp. EGY7 carotenoids on triple-negative breast cancer cells: invitro study |
| title_full | Impact of Paracoccus sp. EGY7 carotenoids on triple-negative breast cancer cells: invitro study |
| title_fullStr | Impact of Paracoccus sp. EGY7 carotenoids on triple-negative breast cancer cells: invitro study |
| title_full_unstemmed | Impact of Paracoccus sp. EGY7 carotenoids on triple-negative breast cancer cells: invitro study |
| title_short | Impact of Paracoccus sp. EGY7 carotenoids on triple-negative breast cancer cells: invitro study |
| title_sort | impact of paracoccus sp egy7 carotenoids on triple negative breast cancer cells invitro study |
| topic | Anticancer Antioxidants Carotenoids Xanthophylls Paracoccus sp. Zeaxanthin. |
| url | https://doi.org/10.1186/s13568-025-01825-5 |
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