A cyanobacteria-derived intermolecular salt bridge stabilizes photosynthetic NDH-1 and prevents oxidative stress
Abstract Throughout evolution, addition of numerous cyanobacteria-derived subunits to the photosynthetic NDH-1 complex stabilizes the complex and facilitates cyclic electron transfer around photosystem I (PSI CET), a critical antioxidant mechanism for efficient photosynthesis, but its stabilization...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07556-4 |
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| author | Mei Zheng Yuanyuan Jiang Zhaoxing Ran Shengjun Liang Tingting Xiao Xiafei Li Weimin Ma |
| author_facet | Mei Zheng Yuanyuan Jiang Zhaoxing Ran Shengjun Liang Tingting Xiao Xiafei Li Weimin Ma |
| author_sort | Mei Zheng |
| collection | DOAJ |
| description | Abstract Throughout evolution, addition of numerous cyanobacteria-derived subunits to the photosynthetic NDH-1 complex stabilizes the complex and facilitates cyclic electron transfer around photosystem I (PSI CET), a critical antioxidant mechanism for efficient photosynthesis, but its stabilization mechanism remains elusive. Here, a cyanobacteria-derived intermolecular salt bridge is found to form between the two conserved subunits, NdhF1 and NdhD1. Its disruption destabilizes photosynthetic NDH-1 and impairs PSI CET, resulting in the production of more reactive oxygen species under high light conditions. The salt bridge and transmembrane helix 16, both situated at the C-terminus of NdhF1, collaboratively secure the linkage between NdhD1 and NdhB, akin to a cramping mechanism. The linkage is also stabilized by cyanobacteria-derived NdhP and NdhQ subunits, but their stabilization mechanisms are distinctly different. Collectively, to the best of our knowledge, this is the first study to unveil the stabilization mechanism of photosynthetic NDH-1 by incorporating photosynthetic components into its conserved subunits during evolution. |
| format | Article |
| id | doaj-art-57b7ddbae06b443abd96fa373ce1a54c |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-57b7ddbae06b443abd96fa373ce1a54c2025-02-09T12:50:19ZengNature PortfolioCommunications Biology2399-36422025-02-018111110.1038/s42003-025-07556-4A cyanobacteria-derived intermolecular salt bridge stabilizes photosynthetic NDH-1 and prevents oxidative stressMei Zheng0Yuanyuan Jiang1Zhaoxing Ran2Shengjun Liang3Tingting Xiao4Xiafei Li5Weimin Ma6College of Life Sciences, Shanghai Normal UniversityCollege of Life Sciences, Shanghai Normal UniversityCollege of Life Sciences, Shanghai Normal UniversityCollege of Life Sciences, Shanghai Normal UniversityCollege of Life Sciences, Shanghai Normal UniversityCollege of Life Sciences, Shanghai Normal UniversityCollege of Life Sciences, Shanghai Normal UniversityAbstract Throughout evolution, addition of numerous cyanobacteria-derived subunits to the photosynthetic NDH-1 complex stabilizes the complex and facilitates cyclic electron transfer around photosystem I (PSI CET), a critical antioxidant mechanism for efficient photosynthesis, but its stabilization mechanism remains elusive. Here, a cyanobacteria-derived intermolecular salt bridge is found to form between the two conserved subunits, NdhF1 and NdhD1. Its disruption destabilizes photosynthetic NDH-1 and impairs PSI CET, resulting in the production of more reactive oxygen species under high light conditions. The salt bridge and transmembrane helix 16, both situated at the C-terminus of NdhF1, collaboratively secure the linkage between NdhD1 and NdhB, akin to a cramping mechanism. The linkage is also stabilized by cyanobacteria-derived NdhP and NdhQ subunits, but their stabilization mechanisms are distinctly different. Collectively, to the best of our knowledge, this is the first study to unveil the stabilization mechanism of photosynthetic NDH-1 by incorporating photosynthetic components into its conserved subunits during evolution.https://doi.org/10.1038/s42003-025-07556-4 |
| spellingShingle | Mei Zheng Yuanyuan Jiang Zhaoxing Ran Shengjun Liang Tingting Xiao Xiafei Li Weimin Ma A cyanobacteria-derived intermolecular salt bridge stabilizes photosynthetic NDH-1 and prevents oxidative stress Communications Biology |
| title | A cyanobacteria-derived intermolecular salt bridge stabilizes photosynthetic NDH-1 and prevents oxidative stress |
| title_full | A cyanobacteria-derived intermolecular salt bridge stabilizes photosynthetic NDH-1 and prevents oxidative stress |
| title_fullStr | A cyanobacteria-derived intermolecular salt bridge stabilizes photosynthetic NDH-1 and prevents oxidative stress |
| title_full_unstemmed | A cyanobacteria-derived intermolecular salt bridge stabilizes photosynthetic NDH-1 and prevents oxidative stress |
| title_short | A cyanobacteria-derived intermolecular salt bridge stabilizes photosynthetic NDH-1 and prevents oxidative stress |
| title_sort | cyanobacteria derived intermolecular salt bridge stabilizes photosynthetic ndh 1 and prevents oxidative stress |
| url | https://doi.org/10.1038/s42003-025-07556-4 |
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