Toxicity of Low-dose Graphene Oxide Nanoparticles in an in-vivo Wild Type of Caenorhabditis elegans Model
Abstract Carbon-based engineered nanomaterials, such as graphene oxide nanoparticles (GO NPs), are widely available for application, but their potentially adverse health effects on humans still require investigation. In this study, the environmental levels of GO NPs are addressed to examine whether...
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| Format: | Article |
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Springer
2020-12-01
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| Series: | Aerosol and Air Quality Research |
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| Online Access: | https://doi.org/10.4209/aaqr.200559 |
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| author | Ming-Hsien Tsai How-Ran Chao Jheng-Jie Jiang Yu-Hsieh Su Mariene-syne P. Cortez Lemmuel L. Tayo I-Cheng Lu Hao Hsieh Chih-Chung Lin Sheng-Lun Lin Wan Nurdiyana Wan Mansor Ching-Kai Su Sen-Ting Huang Wen-Li Hsu |
| author_facet | Ming-Hsien Tsai How-Ran Chao Jheng-Jie Jiang Yu-Hsieh Su Mariene-syne P. Cortez Lemmuel L. Tayo I-Cheng Lu Hao Hsieh Chih-Chung Lin Sheng-Lun Lin Wan Nurdiyana Wan Mansor Ching-Kai Su Sen-Ting Huang Wen-Li Hsu |
| author_sort | Ming-Hsien Tsai |
| collection | DOAJ |
| description | Abstract Carbon-based engineered nanomaterials, such as graphene oxide nanoparticles (GO NPs), are widely available for application, but their potentially adverse health effects on humans still require investigation. In this study, the environmental levels of GO NPs are addressed to examine whether GO leads to adverse effects on an in-vivo model of Caenorhabditis elegans (C. elegans). Nematodes with prolonged exposure (L1 larvae to young adult) to GO NPs at 0.00100, 0.0100, 0.100, and 1.00 μg L−1 were used to evaluate the potential toxic effects, including lethality (acute toxicity), reproductive (brood size) and neurological (locomotion including head thrash and body bend) responses, longevity (lifespan), and oxidative stress (gene expression of sod-1, sod-3, and clt-2). Prolonged exposure to GO NPs was not found to induce lethality at the selective levels. In the brood-size and head-thrash tests, the biological responses in nematodes were significantly reduced at 0.0100-1.00 ng L−1 GO NP exposure as compared with the untreated control. The nematodes exposure to GO NPs at 0.00100–1.00 ng L−1 exhibited significant delays in body bending behavior compared with the control. In the examination of the longevity of nematodes, it was found that the lifespan of all GO NP-exposed worms was significantly shortened as compared to the untreated worms. Gene expression of sod-1, sod-3, and ctl-2 presented significantly higher induction folds in the exposed worms compared with the controls. Consequently, prolonged exposure to the low-dose GO NPs might be associated with disruption of reproduction and locomotion, attenuation of longevity, and induction of oxidative stress in nematodes. |
| format | Article |
| id | doaj-art-58927ba55172467c99c4e7b670b0a0e1 |
| institution | Kabale University |
| issn | 1680-8584 2071-1409 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Springer |
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| series | Aerosol and Air Quality Research |
| spelling | doaj-art-58927ba55172467c99c4e7b670b0a0e12025-02-09T12:20:54ZengSpringerAerosol and Air Quality Research1680-85842071-14092020-12-0121511510.4209/aaqr.200559Toxicity of Low-dose Graphene Oxide Nanoparticles in an in-vivo Wild Type of Caenorhabditis elegans ModelMing-Hsien Tsai0How-Ran Chao1Jheng-Jie Jiang2Yu-Hsieh Su3Mariene-syne P. Cortez4Lemmuel L. Tayo5I-Cheng Lu6Hao Hsieh7Chih-Chung Lin8Sheng-Lun Lin9Wan Nurdiyana Wan Mansor10Ching-Kai Su11Sen-Ting Huang12Wen-Li Hsu13Department of Child Care, National Pingtung University of Science and TechnologyDepartment of Environmental Science and Engineering, National Pingtung University of Science and TechnologyDepartment of Environmental Engineering, Chung Yuan Christian UniversityDepartment of Environmental Science and Engineering, National Pingtung University of Science and TechnologySchool of Chemical, Biological and Materials Engineering and Sciences, Mapúa UniversitySchool of Chemical, Biological and Materials Engineering and Sciences, Mapúa UniversityDepartment of Environmental Science and Engineering, National Pingtung University of Science and TechnologyDepartment of Environmental Science and Engineering, National Pingtung University of Science and TechnologyDepartment of Environmental Science and Engineering, National Pingtung University of Science and TechnologySchool of Mechanical Engineering, Beijing Institute of TechnologyFaculty of Ocean Engineering Technology & Informatics, Universiti Malaysia TerengganuDepartment of Internal Medicine, Kaohsiung Veterans General Hospital Pingtung BranchResearch Institute for Life Support Innovation, Research Organization for Nano and Life Innovation, Waseda UniversityEmerging Compounds Research Center, General Research Service Center, National Pingtung University of Science and TechnologyAbstract Carbon-based engineered nanomaterials, such as graphene oxide nanoparticles (GO NPs), are widely available for application, but their potentially adverse health effects on humans still require investigation. In this study, the environmental levels of GO NPs are addressed to examine whether GO leads to adverse effects on an in-vivo model of Caenorhabditis elegans (C. elegans). Nematodes with prolonged exposure (L1 larvae to young adult) to GO NPs at 0.00100, 0.0100, 0.100, and 1.00 μg L−1 were used to evaluate the potential toxic effects, including lethality (acute toxicity), reproductive (brood size) and neurological (locomotion including head thrash and body bend) responses, longevity (lifespan), and oxidative stress (gene expression of sod-1, sod-3, and clt-2). Prolonged exposure to GO NPs was not found to induce lethality at the selective levels. In the brood-size and head-thrash tests, the biological responses in nematodes were significantly reduced at 0.0100-1.00 ng L−1 GO NP exposure as compared with the untreated control. The nematodes exposure to GO NPs at 0.00100–1.00 ng L−1 exhibited significant delays in body bending behavior compared with the control. In the examination of the longevity of nematodes, it was found that the lifespan of all GO NP-exposed worms was significantly shortened as compared to the untreated worms. Gene expression of sod-1, sod-3, and ctl-2 presented significantly higher induction folds in the exposed worms compared with the controls. Consequently, prolonged exposure to the low-dose GO NPs might be associated with disruption of reproduction and locomotion, attenuation of longevity, and induction of oxidative stress in nematodes.https://doi.org/10.4209/aaqr.200559Graphene oxideCaenorhabditis elegansReproductive toxicityNeurobehavioral toxicityOxidative stress |
| spellingShingle | Ming-Hsien Tsai How-Ran Chao Jheng-Jie Jiang Yu-Hsieh Su Mariene-syne P. Cortez Lemmuel L. Tayo I-Cheng Lu Hao Hsieh Chih-Chung Lin Sheng-Lun Lin Wan Nurdiyana Wan Mansor Ching-Kai Su Sen-Ting Huang Wen-Li Hsu Toxicity of Low-dose Graphene Oxide Nanoparticles in an in-vivo Wild Type of Caenorhabditis elegans Model Aerosol and Air Quality Research Graphene oxide Caenorhabditis elegans Reproductive toxicity Neurobehavioral toxicity Oxidative stress |
| title | Toxicity of Low-dose Graphene Oxide Nanoparticles in an in-vivo Wild Type of Caenorhabditis elegans Model |
| title_full | Toxicity of Low-dose Graphene Oxide Nanoparticles in an in-vivo Wild Type of Caenorhabditis elegans Model |
| title_fullStr | Toxicity of Low-dose Graphene Oxide Nanoparticles in an in-vivo Wild Type of Caenorhabditis elegans Model |
| title_full_unstemmed | Toxicity of Low-dose Graphene Oxide Nanoparticles in an in-vivo Wild Type of Caenorhabditis elegans Model |
| title_short | Toxicity of Low-dose Graphene Oxide Nanoparticles in an in-vivo Wild Type of Caenorhabditis elegans Model |
| title_sort | toxicity of low dose graphene oxide nanoparticles in an in vivo wild type of caenorhabditis elegans model |
| topic | Graphene oxide Caenorhabditis elegans Reproductive toxicity Neurobehavioral toxicity Oxidative stress |
| url | https://doi.org/10.4209/aaqr.200559 |
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