Toxic Assessment of Heavily Traffic-related Fine Particulate Matter Using an in-vivo Wild-type Caenorhabditis elegans Model
Abstract In association with the mortality rate due to air pollution, vehicular emitted fine particles (PM2.5) are a threat to public health. PM2.5-induced in-vivo studies on environmental microorganisms can be used to assess the adverse impacts of PM2.5 on human health. In the present study, the to...
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| Format: | Article |
| Language: | English |
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Springer
2020-06-01
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| Series: | Aerosol and Air Quality Research |
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| Online Access: | https://doi.org/10.4209/aaqr.2020.05.0192 |
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| author | Meng-Ching Chung Kuo-Lin Huang Japheth L. Avelino Lemmuel L. Tayo Chih-Chung Lin Ming-Hsien Tsai Sheng-Lun Lin Wan Nurdiyana Wan Mansor Ching-Kai Su Sen-Ting Huang |
| author_facet | Meng-Ching Chung Kuo-Lin Huang Japheth L. Avelino Lemmuel L. Tayo Chih-Chung Lin Ming-Hsien Tsai Sheng-Lun Lin Wan Nurdiyana Wan Mansor Ching-Kai Su Sen-Ting Huang |
| author_sort | Meng-Ching Chung |
| collection | DOAJ |
| description | Abstract In association with the mortality rate due to air pollution, vehicular emitted fine particles (PM2.5) are a threat to public health. PM2.5-induced in-vivo studies on environmental microorganisms can be used to assess the adverse impacts of PM2.5 on human health. In the present study, the toxicity of traffic-related-air-pollutant (TRAP) PM2.5 was evaluated in the animal model Caenorhabditis elegans (C. elegans) using different toxicological endpoints such as lethality, survivability (lifespan), behavioral (head thrashing and body bending), and reproduction (brood size). The TRAP PM2.5 sample were collected in Taichung City, Taiwan from Mar 24 to April 15 in 2018. Of these 23 day samples, three samples (Days A, B, and C) were randomly selected. The results showed that no immediate lethality was observed after acute (24 h) exposure of the nematodes. On the other hand, sublethal endpoints of reproduction exhibited statistically significant dose-dependent reduction, although Day A and Day C did not decrease the egg-laying capability of the worms. For the neurological toxicity, it is inferred that the higher the PM2.5 concentrations, the more the adverse effects of neurobehavior (head trashing and body bending) it poses on the C. elegans. The lifespans of nematodes exposed to heavily TRAP PM2.5 were significantly shortened compared with those of untreated ones based on survival rate. The nematodes exposed PM2.5 models not only posed potentially adverse health effects on human but also represented ecotoxic impacts on the ecosystem. In conclusion, heavy concentrations of TRAP PM2.5 significantly and severely disrupted toxicological endpoints of neurology and reproduction to C. elegans. TRAP PM2.5 significantly shortened the lifespan of the nematodes compared with the control. TRAP PM2.5 might more severely influenced the specific toxic endpoints, such as lifespan and neurobehavira, in this in-vivo models compared with the reproductive endpoints. |
| format | Article |
| id | doaj-art-598896d4135d47c2ab4b62fbd122b62f |
| institution | Kabale University |
| issn | 1680-8584 2071-1409 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Springer |
| record_format | Article |
| series | Aerosol and Air Quality Research |
| spelling | doaj-art-598896d4135d47c2ab4b62fbd122b62f2025-02-09T12:19:35ZengSpringerAerosol and Air Quality Research1680-85842071-14092020-06-012091974198610.4209/aaqr.2020.05.0192Toxic Assessment of Heavily Traffic-related Fine Particulate Matter Using an in-vivo Wild-type Caenorhabditis elegans ModelMeng-Ching Chung0Kuo-Lin Huang1Japheth L. Avelino2Lemmuel L. Tayo3Chih-Chung Lin4Ming-Hsien Tsai5Sheng-Lun Lin6Wan Nurdiyana Wan Mansor7Ching-Kai Su8Sen-Ting Huang9Department of Environmental Science and Engineering, National Pingtung University of Science and TechnologyDepartment of Environmental Science and Engineering, National Pingtung University of Science and TechnologySchool of Chemical, Biological and Materials Engineering and Sciences, Mapúa UniversitySchool of Chemical, Biological and Materials Engineering and Sciences, Mapúa UniversityDepartment of Environmental Science and Engineering, National Pingtung University of Science and TechnologyDepartment of Child Care, National Pingtung University of Science and TechnologyDepartment of Civil Engineering and Geomatics, Cheng Shiu UniversityFaculty of Ocean Engineering Technology & Informatics, Universiti MalaysiaDepartment of Internal Medicine, Kaohsiung Veterans General Hospital Pingtung BranchDepartment of Internal Medicine, Kaohsiung Veterans General Hospital Pingtung BranchAbstract In association with the mortality rate due to air pollution, vehicular emitted fine particles (PM2.5) are a threat to public health. PM2.5-induced in-vivo studies on environmental microorganisms can be used to assess the adverse impacts of PM2.5 on human health. In the present study, the toxicity of traffic-related-air-pollutant (TRAP) PM2.5 was evaluated in the animal model Caenorhabditis elegans (C. elegans) using different toxicological endpoints such as lethality, survivability (lifespan), behavioral (head thrashing and body bending), and reproduction (brood size). The TRAP PM2.5 sample were collected in Taichung City, Taiwan from Mar 24 to April 15 in 2018. Of these 23 day samples, three samples (Days A, B, and C) were randomly selected. The results showed that no immediate lethality was observed after acute (24 h) exposure of the nematodes. On the other hand, sublethal endpoints of reproduction exhibited statistically significant dose-dependent reduction, although Day A and Day C did not decrease the egg-laying capability of the worms. For the neurological toxicity, it is inferred that the higher the PM2.5 concentrations, the more the adverse effects of neurobehavior (head trashing and body bending) it poses on the C. elegans. The lifespans of nematodes exposed to heavily TRAP PM2.5 were significantly shortened compared with those of untreated ones based on survival rate. The nematodes exposed PM2.5 models not only posed potentially adverse health effects on human but also represented ecotoxic impacts on the ecosystem. In conclusion, heavy concentrations of TRAP PM2.5 significantly and severely disrupted toxicological endpoints of neurology and reproduction to C. elegans. TRAP PM2.5 significantly shortened the lifespan of the nematodes compared with the control. TRAP PM2.5 might more severely influenced the specific toxic endpoints, such as lifespan and neurobehavira, in this in-vivo models compared with the reproductive endpoints.https://doi.org/10.4209/aaqr.2020.05.0192PM2.5Traffic related air pollutant (TRAP)C. elegansLifespanReproductionLocomotion |
| spellingShingle | Meng-Ching Chung Kuo-Lin Huang Japheth L. Avelino Lemmuel L. Tayo Chih-Chung Lin Ming-Hsien Tsai Sheng-Lun Lin Wan Nurdiyana Wan Mansor Ching-Kai Su Sen-Ting Huang Toxic Assessment of Heavily Traffic-related Fine Particulate Matter Using an in-vivo Wild-type Caenorhabditis elegans Model Aerosol and Air Quality Research PM2.5 Traffic related air pollutant (TRAP) C. elegans Lifespan Reproduction Locomotion |
| title | Toxic Assessment of Heavily Traffic-related Fine Particulate Matter Using an in-vivo Wild-type Caenorhabditis elegans Model |
| title_full | Toxic Assessment of Heavily Traffic-related Fine Particulate Matter Using an in-vivo Wild-type Caenorhabditis elegans Model |
| title_fullStr | Toxic Assessment of Heavily Traffic-related Fine Particulate Matter Using an in-vivo Wild-type Caenorhabditis elegans Model |
| title_full_unstemmed | Toxic Assessment of Heavily Traffic-related Fine Particulate Matter Using an in-vivo Wild-type Caenorhabditis elegans Model |
| title_short | Toxic Assessment of Heavily Traffic-related Fine Particulate Matter Using an in-vivo Wild-type Caenorhabditis elegans Model |
| title_sort | toxic assessment of heavily traffic related fine particulate matter using an in vivo wild type caenorhabditis elegans model |
| topic | PM2.5 Traffic related air pollutant (TRAP) C. elegans Lifespan Reproduction Locomotion |
| url | https://doi.org/10.4209/aaqr.2020.05.0192 |
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