Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flight
Abstract Returning to the moon and traveling to Mars represent the main targets of human space exploration missions within the upcoming decades. Comparable to microgravity, partial gravity in these destinations is assumed to dysregulate immune functions, thereby threatening astronauts´ health. To in...
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Nature Portfolio
2025-02-01
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| Series: | npj Microgravity |
| Online Access: | https://doi.org/10.1038/s41526-024-00456-7 |
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| author | Yu Du Bing Han Katharina Biere Nathalie Abdelmalek Xinyu Shu Chaoyang Song Guangyao Chen Ning Li Marina Tuschen Huan Wu Shujin Sun Alexander Choukér Mian Long Dominique Moser |
| author_facet | Yu Du Bing Han Katharina Biere Nathalie Abdelmalek Xinyu Shu Chaoyang Song Guangyao Chen Ning Li Marina Tuschen Huan Wu Shujin Sun Alexander Choukér Mian Long Dominique Moser |
| author_sort | Yu Du |
| collection | DOAJ |
| description | Abstract Returning to the moon and traveling to Mars represent the main targets of human space exploration missions within the upcoming decades. Comparable to microgravity, partial gravity in these destinations is assumed to dysregulate immune functions, thereby threatening astronauts´ health. To investigate the impact of partial gravity on immune cell attachment to vessel endothelia, THP-1 cells and HUVEC cell layers were monitored in a flow chamber system during parabolic flight in lunar (0.16 g) or Martian (0.38 g) gravity. Focus was set on floating speed, cell adhesion, surface molecule expression and cytoskeletal reorganization under basal and TNF-induced inflammatory environment. Floating speed of THP-1 cells was increased in partial gravity, which was accompanied by a successively lower adhesion to the endothelial HUVEC cells. Expression levels of the adhesion markers Mac-1 on THP-1 cells as well as ICAM-1 on HUVECs were found elevated in lunar and Martian gravity, which was aggravated by TNF. Analysis of cytoskeletal organization in HUVECs revealed reduced intracellular F-actin microfilament networks and a stronger cell directionality with stress fiber alignment at cell borders in partial gravity, which was intensified by TNF. In summary, altered immune cell - endothelium interactions as quantified in partial gravity conditions show similarities to cellular behavior in microgravity. However, the different magnitudes of effects in dependence of gravitational level still need to be assessed in further investigations. |
| format | Article |
| id | doaj-art-5b578778eafb4efd9afa252dd79acc29 |
| institution | Kabale University |
| issn | 2373-8065 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Microgravity |
| spelling | doaj-art-5b578778eafb4efd9afa252dd79acc292025-02-09T12:48:37ZengNature Portfolionpj Microgravity2373-80652025-02-011111810.1038/s41526-024-00456-7Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flightYu Du0Bing Han1Katharina Biere2Nathalie Abdelmalek3Xinyu Shu4Chaoyang Song5Guangyao Chen6Ning Li7Marina Tuschen8Huan Wu9Shujin Sun10Alexander Choukér11Mian Long12Dominique Moser13Key Laboratory of Microgravity (National Microgravity Laboratory), Center of Biomechanics and Bioengineering, and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of SciencesLaboratory of Translational Research ‘Stress and Immunity’, Department of Anesthesiology, LMU Hospital, Ludwig-Maximilians-University MunichLaboratory of Translational Research ‘Stress and Immunity’, Department of Anesthesiology, LMU Hospital, Ludwig-Maximilians-University MunichLaboratory of Translational Research ‘Stress and Immunity’, Department of Anesthesiology, LMU Hospital, Ludwig-Maximilians-University MunichKey Laboratory of Microgravity (National Microgravity Laboratory), Center of Biomechanics and Bioengineering, and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of SciencesKey Laboratory of Microgravity (National Microgravity Laboratory), Center of Biomechanics and Bioengineering, and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of SciencesKey Laboratory of Microgravity (National Microgravity Laboratory), Center of Biomechanics and Bioengineering, and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of SciencesKey Laboratory of Microgravity (National Microgravity Laboratory), Center of Biomechanics and Bioengineering, and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of SciencesLaboratory of Translational Research ‘Stress and Immunity’, Department of Anesthesiology, LMU Hospital, Ludwig-Maximilians-University MunichKey Laboratory of Microgravity (National Microgravity Laboratory), Center of Biomechanics and Bioengineering, and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of SciencesKey Laboratory of Microgravity (National Microgravity Laboratory), Center of Biomechanics and Bioengineering, and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of SciencesLaboratory of Translational Research ‘Stress and Immunity’, Department of Anesthesiology, LMU Hospital, Ludwig-Maximilians-University MunichKey Laboratory of Microgravity (National Microgravity Laboratory), Center of Biomechanics and Bioengineering, and Beijing Key Laboratory of Engineered Construction and Mechanobiology, Institute of Mechanics, Chinese Academy of SciencesLaboratory of Translational Research ‘Stress and Immunity’, Department of Anesthesiology, LMU Hospital, Ludwig-Maximilians-University MunichAbstract Returning to the moon and traveling to Mars represent the main targets of human space exploration missions within the upcoming decades. Comparable to microgravity, partial gravity in these destinations is assumed to dysregulate immune functions, thereby threatening astronauts´ health. To investigate the impact of partial gravity on immune cell attachment to vessel endothelia, THP-1 cells and HUVEC cell layers were monitored in a flow chamber system during parabolic flight in lunar (0.16 g) or Martian (0.38 g) gravity. Focus was set on floating speed, cell adhesion, surface molecule expression and cytoskeletal reorganization under basal and TNF-induced inflammatory environment. Floating speed of THP-1 cells was increased in partial gravity, which was accompanied by a successively lower adhesion to the endothelial HUVEC cells. Expression levels of the adhesion markers Mac-1 on THP-1 cells as well as ICAM-1 on HUVECs were found elevated in lunar and Martian gravity, which was aggravated by TNF. Analysis of cytoskeletal organization in HUVECs revealed reduced intracellular F-actin microfilament networks and a stronger cell directionality with stress fiber alignment at cell borders in partial gravity, which was intensified by TNF. In summary, altered immune cell - endothelium interactions as quantified in partial gravity conditions show similarities to cellular behavior in microgravity. However, the different magnitudes of effects in dependence of gravitational level still need to be assessed in further investigations.https://doi.org/10.1038/s41526-024-00456-7 |
| spellingShingle | Yu Du Bing Han Katharina Biere Nathalie Abdelmalek Xinyu Shu Chaoyang Song Guangyao Chen Ning Li Marina Tuschen Huan Wu Shujin Sun Alexander Choukér Mian Long Dominique Moser Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flight npj Microgravity |
| title | Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flight |
| title_full | Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flight |
| title_fullStr | Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flight |
| title_full_unstemmed | Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flight |
| title_short | Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flight |
| title_sort | lunar and martian gravity alter immune cell interactions with endothelia in parabolic flight |
| url | https://doi.org/10.1038/s41526-024-00456-7 |
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