Deciphering the anti-obesity mechanisms of pharmabiotic probiotics through advanced multiomics analysis
Summary: Probiotics with “pharmabiotic” properties are increasingly recognized as effective tools for combating obesity by altering gut microbiota and reducing body fat. However, the molecular mechanisms underlying their anti-obesity effects remain largely unexplored due to the absence of a universa...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225001506 |
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| author | Ye-Rim Kim Tae-Rim Choi Sung-Hyun Jo Won-Suk Song TaeHyun Kim Min-Gyu Kim Ji-Hyun Baek Seo-Young Kwon Bo-Gyeong Choi Sang Woo Seo Chol-Soon Jang Yung-Hun Yang Yun-Gon Kim |
| author_facet | Ye-Rim Kim Tae-Rim Choi Sung-Hyun Jo Won-Suk Song TaeHyun Kim Min-Gyu Kim Ji-Hyun Baek Seo-Young Kwon Bo-Gyeong Choi Sang Woo Seo Chol-Soon Jang Yung-Hun Yang Yun-Gon Kim |
| author_sort | Ye-Rim Kim |
| collection | DOAJ |
| description | Summary: Probiotics with “pharmabiotic” properties are increasingly recognized as effective tools for combating obesity by altering gut microbiota and reducing body fat. However, the molecular mechanisms underlying their anti-obesity effects remain largely unexplored due to the absence of a universal methodology. Herein, we developed a multiomics-based strategy to elucidate how probiotics reduce lipid production in adipocytes. Our initial investigation assessed the impact of probiotics at defined adipocyte differentiation stages. Leveraging these insights, we performed comprehensive multiomics analyses at key intervals to identify the suppression mechanisms of lipid formation. Lactobacillus reuteri, specifically, targets early differentiation stages, inhibits branched-chain amino acid catabolism, and reduces lipid accumulation in adipocytes by suppressing Krüppel-like factor 5. Concurrently, enhanced hypoxia-inducible factor 1 expression impedes adipogenesis by downregulating lipin-1 expression. This study not only demonstrates the effectiveness of our approach in revealing complex host-microbe interactions but also significantly advances probiotic therapeutic development, offering promising avenues for obesity management. |
| format | Article |
| id | doaj-art-5bd659fd456c4c2499b5680e59a55007 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-5bd659fd456c4c2499b5680e59a550072025-02-11T04:35:20ZengElsevieriScience2589-00422025-02-01282111890Deciphering the anti-obesity mechanisms of pharmabiotic probiotics through advanced multiomics analysisYe-Rim Kim0Tae-Rim Choi1Sung-Hyun Jo2Won-Suk Song3TaeHyun Kim4Min-Gyu Kim5Ji-Hyun Baek6Seo-Young Kwon7Bo-Gyeong Choi8Sang Woo Seo9Chol-Soon Jang10Yung-Hun Yang11Yun-Gon Kim12Department of Chemical Engineering, Soongsil University, Seoul 06978, Republic of KoreaDepartment of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of KoreaDepartment of Chemical Engineering, Soongsil University, Seoul 06978, Republic of KoreaDepartment of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USASchool of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of KoreaDepartment of Chemical Engineering, Soongsil University, Seoul 06978, Republic of KoreaDepartment of Chemical Engineering, Soongsil University, Seoul 06978, Republic of KoreaDepartment of Chemical Engineering, Soongsil University, Seoul 06978, Republic of KoreaDepartment of Chemical Engineering, Soongsil University, Seoul 06978, Republic of KoreaSchool of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of KoreaSchool of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of KoreaDepartment of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of KoreaDepartment of Chemical Engineering, Soongsil University, Seoul 06978, Republic of Korea; Corresponding authorSummary: Probiotics with “pharmabiotic” properties are increasingly recognized as effective tools for combating obesity by altering gut microbiota and reducing body fat. However, the molecular mechanisms underlying their anti-obesity effects remain largely unexplored due to the absence of a universal methodology. Herein, we developed a multiomics-based strategy to elucidate how probiotics reduce lipid production in adipocytes. Our initial investigation assessed the impact of probiotics at defined adipocyte differentiation stages. Leveraging these insights, we performed comprehensive multiomics analyses at key intervals to identify the suppression mechanisms of lipid formation. Lactobacillus reuteri, specifically, targets early differentiation stages, inhibits branched-chain amino acid catabolism, and reduces lipid accumulation in adipocytes by suppressing Krüppel-like factor 5. Concurrently, enhanced hypoxia-inducible factor 1 expression impedes adipogenesis by downregulating lipin-1 expression. This study not only demonstrates the effectiveness of our approach in revealing complex host-microbe interactions but also significantly advances probiotic therapeutic development, offering promising avenues for obesity management.http://www.sciencedirect.com/science/article/pii/S2589004225001506Obesity medicineMicrobiologyMicrobiomeOmics |
| spellingShingle | Ye-Rim Kim Tae-Rim Choi Sung-Hyun Jo Won-Suk Song TaeHyun Kim Min-Gyu Kim Ji-Hyun Baek Seo-Young Kwon Bo-Gyeong Choi Sang Woo Seo Chol-Soon Jang Yung-Hun Yang Yun-Gon Kim Deciphering the anti-obesity mechanisms of pharmabiotic probiotics through advanced multiomics analysis iScience Obesity medicine Microbiology Microbiome Omics |
| title | Deciphering the anti-obesity mechanisms of pharmabiotic probiotics through advanced multiomics analysis |
| title_full | Deciphering the anti-obesity mechanisms of pharmabiotic probiotics through advanced multiomics analysis |
| title_fullStr | Deciphering the anti-obesity mechanisms of pharmabiotic probiotics through advanced multiomics analysis |
| title_full_unstemmed | Deciphering the anti-obesity mechanisms of pharmabiotic probiotics through advanced multiomics analysis |
| title_short | Deciphering the anti-obesity mechanisms of pharmabiotic probiotics through advanced multiomics analysis |
| title_sort | deciphering the anti obesity mechanisms of pharmabiotic probiotics through advanced multiomics analysis |
| topic | Obesity medicine Microbiology Microbiome Omics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225001506 |
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