The unique activity of the bone morphogenetic protein TGH4 affects the embryonic development of Trichinella spiralis and the establishment of vaccine protection
Abstract In recent years, animal outbreaks of trichinellosis have been reported in Mexico, China, Algeria, Croatia and others, which is a challenge to meat safety and public health. Vaccination is an important means to control trichinellosis, but one of the main challenges in vaccine development is...
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-025-01473-4 |
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| author | Wenjie Shi Yi Liu Yan Liu Xue Bai Yue Liang Yaming Yang Fangwei Wu Mingyuan Liu Ning Xu |
| author_facet | Wenjie Shi Yi Liu Yan Liu Xue Bai Yue Liang Yaming Yang Fangwei Wu Mingyuan Liu Ning Xu |
| author_sort | Wenjie Shi |
| collection | DOAJ |
| description | Abstract In recent years, animal outbreaks of trichinellosis have been reported in Mexico, China, Algeria, Croatia and others, which is a challenge to meat safety and public health. Vaccination is an important means to control trichinellosis, but one of the main challenges in vaccine development is screening for protective candidate antigens. Bone morphogenetic proteins (BMP)), which are potential vaccine research targets, play key roles in the growth and development of metazoans. The BMP homologue TGH4 was identified from Trichinella spiralis (T. spiralis), and recombinant C-terminal mature rTGH4-m had BMP activity and exerted regulatory effects on both mouse fibroblast and T. spiralis embryonic development. The construction of a protein mutant without activation potential confirmed that BMP activity had a negative regulatory effect on the establishment of immune protection by affecting the innate, adaptive, and humoral immunity of mice. Stripping protein activity can enhance immune protection and host resistance to T. spiralis. Our findings demonstrate that the TGH4 mutant is an important vaccine candidate antigen that blocks embryonic development, kills larvae, and provides insight into parasite vaccine research. |
| format | Article |
| id | doaj-art-5c3b253dadcd408a99749ee24d4094b4 |
| institution | Kabale University |
| issn | 1297-9716 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj-art-5c3b253dadcd408a99749ee24d4094b42025-02-09T12:50:04ZengBMCVeterinary Research1297-97162025-02-0156111410.1186/s13567-025-01473-4The unique activity of the bone morphogenetic protein TGH4 affects the embryonic development of Trichinella spiralis and the establishment of vaccine protectionWenjie Shi0Yi Liu1Yan Liu2Xue Bai3Yue Liang4Yaming Yang5Fangwei Wu6Mingyuan Liu7Ning Xu8State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin UniversityCollege of Food Science and Engineering, Jilin UniversityCollege of Public Health, Jilin Medical UniversityState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin UniversityState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin UniversityDepartment of Helminth, Yunnan Institute of Parasitic DiseasesDepartment of Helminth, Yunnan Institute of Parasitic DiseasesState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin UniversityState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin UniversityAbstract In recent years, animal outbreaks of trichinellosis have been reported in Mexico, China, Algeria, Croatia and others, which is a challenge to meat safety and public health. Vaccination is an important means to control trichinellosis, but one of the main challenges in vaccine development is screening for protective candidate antigens. Bone morphogenetic proteins (BMP)), which are potential vaccine research targets, play key roles in the growth and development of metazoans. The BMP homologue TGH4 was identified from Trichinella spiralis (T. spiralis), and recombinant C-terminal mature rTGH4-m had BMP activity and exerted regulatory effects on both mouse fibroblast and T. spiralis embryonic development. The construction of a protein mutant without activation potential confirmed that BMP activity had a negative regulatory effect on the establishment of immune protection by affecting the innate, adaptive, and humoral immunity of mice. Stripping protein activity can enhance immune protection and host resistance to T. spiralis. Our findings demonstrate that the TGH4 mutant is an important vaccine candidate antigen that blocks embryonic development, kills larvae, and provides insight into parasite vaccine research.https://doi.org/10.1186/s13567-025-01473-4Trichinella spiralisbone morphogenetic proteinvaccineimmunoregulationprotective efficacy |
| spellingShingle | Wenjie Shi Yi Liu Yan Liu Xue Bai Yue Liang Yaming Yang Fangwei Wu Mingyuan Liu Ning Xu The unique activity of the bone morphogenetic protein TGH4 affects the embryonic development of Trichinella spiralis and the establishment of vaccine protection Veterinary Research Trichinella spiralis bone morphogenetic protein vaccine immunoregulation protective efficacy |
| title | The unique activity of the bone morphogenetic protein TGH4 affects the embryonic development of Trichinella spiralis and the establishment of vaccine protection |
| title_full | The unique activity of the bone morphogenetic protein TGH4 affects the embryonic development of Trichinella spiralis and the establishment of vaccine protection |
| title_fullStr | The unique activity of the bone morphogenetic protein TGH4 affects the embryonic development of Trichinella spiralis and the establishment of vaccine protection |
| title_full_unstemmed | The unique activity of the bone morphogenetic protein TGH4 affects the embryonic development of Trichinella spiralis and the establishment of vaccine protection |
| title_short | The unique activity of the bone morphogenetic protein TGH4 affects the embryonic development of Trichinella spiralis and the establishment of vaccine protection |
| title_sort | unique activity of the bone morphogenetic protein tgh4 affects the embryonic development of trichinella spiralis and the establishment of vaccine protection |
| topic | Trichinella spiralis bone morphogenetic protein vaccine immunoregulation protective efficacy |
| url | https://doi.org/10.1186/s13567-025-01473-4 |
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