The effect of silymarin on ischemia-reperfusion injury in skeletal muscles of rats
Aim: Although the precise process is not entirely elucidated, it is well-known that oxidative stress mediators contribute to ischemia-reperfusion (I/R) injury. The impact of silymarin on I/R injury in many different tissues has been examined. For this purpose, we planned to see the effect of silymar...
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| Format: | Article |
| Language: | English |
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Turkish National Vascular and Endovascular Surgery Society
2024-03-01
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| Series: | Turkish Journal of Vascular Surgery |
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| Online Access: | https://turkishjournalofvascularsurgery.org//?mno=220428 |
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| author | Abdullah Ozer Basak Kocak Mustafa Arslan Baris Mardin Aysegul Kucuk Saban Cem Sezen Mustafa Hakan Zor Mustafa Kavutcu |
| author_facet | Abdullah Ozer Basak Kocak Mustafa Arslan Baris Mardin Aysegul Kucuk Saban Cem Sezen Mustafa Hakan Zor Mustafa Kavutcu |
| author_sort | Abdullah Ozer |
| collection | DOAJ |
| description | Aim: Although the precise process is not entirely elucidated, it is well-known that oxidative stress mediators contribute to ischemia-reperfusion (I/R) injury. The impact of silymarin on I/R injury in many different tissues has been examined. For this purpose, we planned to see the effect of silymarin on muscle tissue in rats subjected to lower extremity I/R injury.
Material and Methods: We used 18 male Wistar albino rats weighing 225-275 g. Each of the three groups of rats [Control (C), Ischemia-Reperfusion (I/R), and Silymarin-Ischemia/Reperfusion (S-I/R)] consisted of six rats. Silymarin was administered intraperitoneally 30 minutes before the procedure. (100 mg/kg-1) In Group I/R, the infrarenal abdominal aorta was clamped with a microvascular clamp. The clamp was removed after 120 minutes, and reperfusion was achieved for the next 120 minutes. At the end of the reperfusion period, muscle tissue samples were collected, and Malondialdehyde (MDA), catalase (CAT) enzyme activity levels and histopathological parameters were compared.
Results: In the histopathological examination, no degeneration was observed in the muscle fibers in the Group C, while findings of striated muscle damage such as muscle atrophy/hypertrophy, muscle degeneration/congestion, internalization of the muscle nucleus/oval/central nucleus, fragmentation/hyalinization and leukocyte cell infiltration were seen in the Group I/R. In Group S-I/R, muscle atrophy /hypertrophy, internalization of the muscle nucleus/oval/central nucleus, fragmentation/hyalinization, and leukocyte cell infiltration were observed to improve these damaged areas compared to Group I/R. MDA levels in the Group I/R were significantly higher compared to Group C and S-I/R. The activity of the CAT enzyme was much higher in Group I/R compared to Group C.
Conclusion: Our study revealed that 100 mg/kg-1 silymarin administered by intraperitoneal injection 30 minutes before ischemia effectively decreased lipid peroxidation, oxidative stress, and the injury caused by I/R in muscle histology in rats. [Turk J Vasc Surg 2024; 33(3.000): 171-5] |
| format | Article |
| id | doaj-art-5d86887c178a49e78985143f3ade90ce |
| institution | Kabale University |
| issn | 2667-5080 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Turkish National Vascular and Endovascular Surgery Society |
| record_format | Article |
| series | Turkish Journal of Vascular Surgery |
| spelling | doaj-art-5d86887c178a49e78985143f3ade90ce2025-02-11T11:13:16ZengTurkish National Vascular and Endovascular Surgery SocietyTurkish Journal of Vascular Surgery2667-50802024-03-013331715220428The effect of silymarin on ischemia-reperfusion injury in skeletal muscles of ratsAbdullah Ozer0Basak Kocak1Mustafa Arslan2Baris Mardin3Aysegul Kucuk4Saban Cem Sezen5Mustafa Hakan Zor6Mustafa Kavutcu71Department of Cardiovascular Surgery, Gazi University Medical Faculty, Ankara, 06510, Turkey 1Department of Cardiovascular Surgery, Gazi University Medical Faculty, Ankara, 06510, Turkey 2Department of Anaesthesiology and Reanimation, Gazi University Medical Faculty, Ankara, 06510, Turkey Department of Cardiovascular Surgery, Gazi University Medical Faculty, Ankara, 06510, Turkey Department of Physiology, Kutahya Health Sciences University Faculty of Medicine, Kutahya, 43020, Turkey 4Department of Histology and Embryology, Kırıkkale University, Kırıkkale, 71450, Turkey Department of Cardiovascular Surgery, Gazi University Medical Faculty, Ankara, 06510, Turkey Department of Medical Biochemistry, Gazi University Medical Faculty, Ankara, 06510, TurkeyAim: Although the precise process is not entirely elucidated, it is well-known that oxidative stress mediators contribute to ischemia-reperfusion (I/R) injury. The impact of silymarin on I/R injury in many different tissues has been examined. For this purpose, we planned to see the effect of silymarin on muscle tissue in rats subjected to lower extremity I/R injury. Material and Methods: We used 18 male Wistar albino rats weighing 225-275 g. Each of the three groups of rats [Control (C), Ischemia-Reperfusion (I/R), and Silymarin-Ischemia/Reperfusion (S-I/R)] consisted of six rats. Silymarin was administered intraperitoneally 30 minutes before the procedure. (100 mg/kg-1) In Group I/R, the infrarenal abdominal aorta was clamped with a microvascular clamp. The clamp was removed after 120 minutes, and reperfusion was achieved for the next 120 minutes. At the end of the reperfusion period, muscle tissue samples were collected, and Malondialdehyde (MDA), catalase (CAT) enzyme activity levels and histopathological parameters were compared. Results: In the histopathological examination, no degeneration was observed in the muscle fibers in the Group C, while findings of striated muscle damage such as muscle atrophy/hypertrophy, muscle degeneration/congestion, internalization of the muscle nucleus/oval/central nucleus, fragmentation/hyalinization and leukocyte cell infiltration were seen in the Group I/R. In Group S-I/R, muscle atrophy /hypertrophy, internalization of the muscle nucleus/oval/central nucleus, fragmentation/hyalinization, and leukocyte cell infiltration were observed to improve these damaged areas compared to Group I/R. MDA levels in the Group I/R were significantly higher compared to Group C and S-I/R. The activity of the CAT enzyme was much higher in Group I/R compared to Group C. Conclusion: Our study revealed that 100 mg/kg-1 silymarin administered by intraperitoneal injection 30 minutes before ischemia effectively decreased lipid peroxidation, oxidative stress, and the injury caused by I/R in muscle histology in rats. [Turk J Vasc Surg 2024; 33(3.000): 171-5]https://turkishjournalofvascularsurgery.org//?mno=220428silymarinmalondialdehydecatalaseischemia-reperfusion |
| spellingShingle | Abdullah Ozer Basak Kocak Mustafa Arslan Baris Mardin Aysegul Kucuk Saban Cem Sezen Mustafa Hakan Zor Mustafa Kavutcu The effect of silymarin on ischemia-reperfusion injury in skeletal muscles of rats Turkish Journal of Vascular Surgery silymarin malondialdehyde catalase ischemia-reperfusion |
| title | The effect of silymarin on ischemia-reperfusion injury in skeletal muscles of rats |
| title_full | The effect of silymarin on ischemia-reperfusion injury in skeletal muscles of rats |
| title_fullStr | The effect of silymarin on ischemia-reperfusion injury in skeletal muscles of rats |
| title_full_unstemmed | The effect of silymarin on ischemia-reperfusion injury in skeletal muscles of rats |
| title_short | The effect of silymarin on ischemia-reperfusion injury in skeletal muscles of rats |
| title_sort | effect of silymarin on ischemia reperfusion injury in skeletal muscles of rats |
| topic | silymarin malondialdehyde catalase ischemia-reperfusion |
| url | https://turkishjournalofvascularsurgery.org//?mno=220428 |
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