Cost-reduction strategy to culture patient derived bladder tumor organoids
Abstract Organoids as self-organized structure derived from stem cells can recapitulate the function of an organ in miniature form which have developed great potential for clinical translation, drug screening and personalized medicine. Nevertheless, the majority of patient-derived organoids (PDOs) a...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-87509-3 |
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| author | Mahsa Mollapour Sisakht Fatemeh Gholizadeh Shirin Hekmatirad Tokameh Mahmoudi Saeed Montazeri Laleh Sharifi Hamed Daemi Shahla Romal Mohammad Hosein Yazdi Mohammad Ali Faramarzi Ahmad Reza Shahverdi Amir Ali Hamidieh |
| author_facet | Mahsa Mollapour Sisakht Fatemeh Gholizadeh Shirin Hekmatirad Tokameh Mahmoudi Saeed Montazeri Laleh Sharifi Hamed Daemi Shahla Romal Mohammad Hosein Yazdi Mohammad Ali Faramarzi Ahmad Reza Shahverdi Amir Ali Hamidieh |
| author_sort | Mahsa Mollapour Sisakht |
| collection | DOAJ |
| description | Abstract Organoids as self-organized structure derived from stem cells can recapitulate the function of an organ in miniature form which have developed great potential for clinical translation, drug screening and personalized medicine. Nevertheless, the majority of patient-derived organoids (PDOs) are currently being cultured in the basement membrane matrices (BMMs), which are constrained by xenogeneic origin, batch-to-batch variability, cost, and complexity. Besides, organoid culture relies on biochemical signals provided by various growth factors in the composition of medium. We propose sodium alginate hydrogel scaffold in addition to the fibroblast conditioned medium (FCM)-enriched culture medium that is inexpensive and easily amenable to clinical applications for the culture of bladder cancer PDOs. PDOs grown in sodium alginate and FCM based medium have proliferation potential, growth rate, and gene expression that are similar to PDOs cultured in BME. According to the results, sodium alginate has substantial mechanical properties and reduces variance in early passage bladder tumor organoid cultures collected from patients. Furthermore, using FCM based medium as an alternative solution to eliminate some essential growth factors can be considered, especially for low-resource situation and develop cost effective tumor organoids. |
| format | Article |
| id | doaj-art-60ecaa1dd4c445a089a30b64ecc55697 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-60ecaa1dd4c445a089a30b64ecc556972025-02-09T12:33:04ZengNature PortfolioScientific Reports2045-23222025-02-0115111610.1038/s41598-025-87509-3Cost-reduction strategy to culture patient derived bladder tumor organoidsMahsa Mollapour Sisakht0Fatemeh Gholizadeh1Shirin Hekmatirad2Tokameh Mahmoudi3Saeed Montazeri4Laleh Sharifi5Hamed Daemi6Shahla Romal7Mohammad Hosein Yazdi8Mohammad Ali Faramarzi9Ahmad Reza Shahverdi10Amir Ali Hamidieh11Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical SciencesStem Cell and Regenerative Medicine innovation center, Tehran University of Medical SciencesStem Cell and Regenerative Medicine innovation center, Tehran University of Medical SciencesDepartment of Urology, Erasmus MC Cancer Institute, Erasmus University Medical Center RotterdamUro-oncology Research Center, Tehran University of Medical SciencesUro-oncology Research Center, Tehran University of Medical SciencesDepartment of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECRDepartment of Urology, Erasmus MC Cancer Institute, Erasmus University Medical Center RotterdamBiotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical SciencesBiotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical SciencesBiotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical SciencesBiotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical SciencesAbstract Organoids as self-organized structure derived from stem cells can recapitulate the function of an organ in miniature form which have developed great potential for clinical translation, drug screening and personalized medicine. Nevertheless, the majority of patient-derived organoids (PDOs) are currently being cultured in the basement membrane matrices (BMMs), which are constrained by xenogeneic origin, batch-to-batch variability, cost, and complexity. Besides, organoid culture relies on biochemical signals provided by various growth factors in the composition of medium. We propose sodium alginate hydrogel scaffold in addition to the fibroblast conditioned medium (FCM)-enriched culture medium that is inexpensive and easily amenable to clinical applications for the culture of bladder cancer PDOs. PDOs grown in sodium alginate and FCM based medium have proliferation potential, growth rate, and gene expression that are similar to PDOs cultured in BME. According to the results, sodium alginate has substantial mechanical properties and reduces variance in early passage bladder tumor organoid cultures collected from patients. Furthermore, using FCM based medium as an alternative solution to eliminate some essential growth factors can be considered, especially for low-resource situation and develop cost effective tumor organoids.https://doi.org/10.1038/s41598-025-87509-3OrganoidsBladder tumorAlginateFibroblast conditioned medium |
| spellingShingle | Mahsa Mollapour Sisakht Fatemeh Gholizadeh Shirin Hekmatirad Tokameh Mahmoudi Saeed Montazeri Laleh Sharifi Hamed Daemi Shahla Romal Mohammad Hosein Yazdi Mohammad Ali Faramarzi Ahmad Reza Shahverdi Amir Ali Hamidieh Cost-reduction strategy to culture patient derived bladder tumor organoids Scientific Reports Organoids Bladder tumor Alginate Fibroblast conditioned medium |
| title | Cost-reduction strategy to culture patient derived bladder tumor organoids |
| title_full | Cost-reduction strategy to culture patient derived bladder tumor organoids |
| title_fullStr | Cost-reduction strategy to culture patient derived bladder tumor organoids |
| title_full_unstemmed | Cost-reduction strategy to culture patient derived bladder tumor organoids |
| title_short | Cost-reduction strategy to culture patient derived bladder tumor organoids |
| title_sort | cost reduction strategy to culture patient derived bladder tumor organoids |
| topic | Organoids Bladder tumor Alginate Fibroblast conditioned medium |
| url | https://doi.org/10.1038/s41598-025-87509-3 |
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