Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?
OBJECTIVE: In this study, we aimed to investigate the role of the cumulus cell’s apoptosis parameter in the maturation of immature rescue oocytes. STUDY DESIGN: In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matu...
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| Format: | Article |
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Medical Network
2021-12-01
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| Series: | Gynecology Obstetrics & Reproductive Medicine |
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| Online Access: | https://gorm.com.tr/index.php/GORM/article/view/1087 |
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| author | Tulay Irez Sinem Ercan Dogan Enver Ciraci Saadet Busra Aksoyer Muhammet Sait Toprak Nurten Dayioglu Onur Guralp |
| author_facet | Tulay Irez Sinem Ercan Dogan Enver Ciraci Saadet Busra Aksoyer Muhammet Sait Toprak Nurten Dayioglu Onur Guralp |
| author_sort | Tulay Irez |
| collection | DOAJ |
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OBJECTIVE: In this study, we aimed to investigate the role of the cumulus cell’s apoptosis parameter in the maturation of immature rescue oocytes.
STUDY DESIGN: In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matured germinal vesicle oocytes were compared with apoptotic properties of cumulus cells.
RESULTS: In all of the immature oocytes after oocyte in vitro maturation, the maturation rate has been observed as 56.1% and 2PN rate as 63.0%. Afterin vitro maturation of germinal vesicle oocytes, there was no difference in apoptosis rates of the cumulus cells between mature and immature oocytes (p> 0.05). The ratio of 2PN in matured germinal vesicle oocytes showing embryo development was 35.4%. A positive correlation was found between luteinizing hormone values on day 3 and E2 values during HCG days during oocyte maturation and embryo development (p=0.021, p=0.020). In addition, it has been observed that the germinal vesicle oocytes, which have completed their maturation and developed into embryos, have high E2 values during HCG days (p=0.020).
CONCLUSION: In our study, it has been demonstrated that in vitro maturation in rescue oocytes from stimulated cycles, embryo development potential could not be explained by the apoptosis parameter.
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| format | Article |
| id | doaj-art-61cecbe505f040b3a083475750c7f0c1 |
| institution | Kabale University |
| issn | 1300-4751 2602-4918 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Medical Network |
| record_format | Article |
| series | Gynecology Obstetrics & Reproductive Medicine |
| spelling | doaj-art-61cecbe505f040b3a083475750c7f0c12025-02-11T21:13:47ZengMedical NetworkGynecology Obstetrics & Reproductive Medicine1300-47512602-49182021-12-0127310.21613/GORM.2020.1087Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?Tulay Irez0Sinem Ercan Dogan1Enver Ciraci2Saadet Busra AksoyerMuhammet Sait Toprak3Nurten DayiogluOnur GuralpIstanbul Yeni Yuzyil University Faculty of Medicine,Dept.of Histology&EmbryologyIstanbul Yeni Yuzyil University ,Histology and Embryology, Clinical Embryology Master program,IstanbulBiruni University ,Health Science Faculty, IstanbulBiruni University Health Science Faculty,Istanbul OBJECTIVE: In this study, we aimed to investigate the role of the cumulus cell’s apoptosis parameter in the maturation of immature rescue oocytes. STUDY DESIGN: In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matured germinal vesicle oocytes were compared with apoptotic properties of cumulus cells. RESULTS: In all of the immature oocytes after oocyte in vitro maturation, the maturation rate has been observed as 56.1% and 2PN rate as 63.0%. Afterin vitro maturation of germinal vesicle oocytes, there was no difference in apoptosis rates of the cumulus cells between mature and immature oocytes (p> 0.05). The ratio of 2PN in matured germinal vesicle oocytes showing embryo development was 35.4%. A positive correlation was found between luteinizing hormone values on day 3 and E2 values during HCG days during oocyte maturation and embryo development (p=0.021, p=0.020). In addition, it has been observed that the germinal vesicle oocytes, which have completed their maturation and developed into embryos, have high E2 values during HCG days (p=0.020). CONCLUSION: In our study, it has been demonstrated that in vitro maturation in rescue oocytes from stimulated cycles, embryo development potential could not be explained by the apoptosis parameter. https://gorm.com.tr/index.php/GORM/article/view/1087ApoptosisCumulus cellsIn vitro oocyte maturation humanTunel assay |
| spellingShingle | Tulay Irez Sinem Ercan Dogan Enver Ciraci Saadet Busra Aksoyer Muhammet Sait Toprak Nurten Dayioglu Onur Guralp Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation? Gynecology Obstetrics & Reproductive Medicine Apoptosis Cumulus cells In vitro oocyte maturation human Tunel assay |
| title | Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation? |
| title_full | Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation? |
| title_fullStr | Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation? |
| title_full_unstemmed | Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation? |
| title_short | Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation? |
| title_sort | does the apoptosis value of cumulus cells play a role in rescue oocyte in vitro maturation |
| topic | Apoptosis Cumulus cells In vitro oocyte maturation human Tunel assay |
| url | https://gorm.com.tr/index.php/GORM/article/view/1087 |
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