Helicobacter pylori CagA+ strains modulate colorectal pathology by regulating intestinal flora
Abstract Aim This article aims to investigate the role of Helicobacter Pylori (HP) CagA+ strains affected colorectal lesion via gut microbiota. Method 6-week C57BL/6J mice were divided into: (a) HP CagA+ group undergoing HP CagA+ strains administration by gavage at 0.2 mL for 10 days; (b) HP CagA- g...
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | BMC Gastroenterology |
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| Online Access: | https://doi.org/10.1186/s12876-025-03631-6 |
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| author | Shasha Cui Xinqiang Liu Fengxia Han Lu Zhang Jingjing Bu Sainan Wu Jiafen Wang |
| author_facet | Shasha Cui Xinqiang Liu Fengxia Han Lu Zhang Jingjing Bu Sainan Wu Jiafen Wang |
| author_sort | Shasha Cui |
| collection | DOAJ |
| description | Abstract Aim This article aims to investigate the role of Helicobacter Pylori (HP) CagA+ strains affected colorectal lesion via gut microbiota. Method 6-week C57BL/6J mice were divided into: (a) HP CagA+ group undergoing HP CagA+ strains administration by gavage at 0.2 mL for 10 days; (b) HP CagA- group undergoing HP CagA- strains administration by gavage at 0.2 mL for 10 days; (c) control group intragastrically given 0.2 mL of brian heart infusion (BHI) medium for 10 days. Gastric mucosa was collected for Giemsa staining, and colorectal mucosa was for hematoxylin and eosin (H&E) staining, 16 S ribosomal RNA (rRNA) sequencing and immunohistochemistry for Major Histocompatibility Complex (MHC). Colon tissues and serum from caudal vein was collected for quantification of interleukin (IL)-6, IL-8, IL10 and tumor necrosis factor (TNF-α). Results Mice with HP CagA+ infection developed loss of some resident cells and inflammation infiltration in colorectal mucosa, and increased Giemsa-positive cells in gastric tissue. Also, MHC II-positive cells were increased in colorectal tissue in HP CagA+ strains infection. HP CagA+ infection cause increase of TNF-α, IL-6, IL-8 and IL-10 in the serum. Meanwhile, HP CagA+ stainis evoked gut microbiota dysbiosis which was characterized by altered microbiome distribution, reduction in Front-to-Back (F/B ratio), decreased α-diversity metric (Chao1 and Shannon). In β-diversity, gut microbiota in control and HP CagA+ groups showed the significant distance based on UniFrac distance. Cag group was enriched a higher abundance of Staphylococcus and Corynebacterium, while control subjects were enriched in Marinifilaceae and Odoribacter. Conclusion HP CagA+ strains are capable of causing gut microbiota dysbiosis to develop destruction of intestinal barrier, and it may affect the development of colorectal cancer by increasing colonization of Staphylococcus and Corynebacterium. |
| format | Article |
| id | doaj-art-64d0cf43ad7645639d40bf874f92368c |
| institution | Kabale University |
| issn | 1471-230X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj-art-64d0cf43ad7645639d40bf874f92368c2025-02-09T12:39:43ZengBMCBMC Gastroenterology1471-230X2025-02-012511910.1186/s12876-025-03631-6Helicobacter pylori CagA+ strains modulate colorectal pathology by regulating intestinal floraShasha Cui0Xinqiang Liu1Fengxia Han2Lu Zhang3Jingjing Bu4Sainan Wu5Jiafen Wang6Clinical Laboratory, Binzhou People’s HospitalDepartment of Oncology, Binzhou People’s HospitalClinical Laboratory, Binzhou People’s HospitalDepartment of Oncology, Binzhou People’s HospitalClinical Laboratory, Binzhou People’s HospitalClinical Laboratory, Binzhou People’s HospitalClinical Laboratory, Binzhou People’s HospitalAbstract Aim This article aims to investigate the role of Helicobacter Pylori (HP) CagA+ strains affected colorectal lesion via gut microbiota. Method 6-week C57BL/6J mice were divided into: (a) HP CagA+ group undergoing HP CagA+ strains administration by gavage at 0.2 mL for 10 days; (b) HP CagA- group undergoing HP CagA- strains administration by gavage at 0.2 mL for 10 days; (c) control group intragastrically given 0.2 mL of brian heart infusion (BHI) medium for 10 days. Gastric mucosa was collected for Giemsa staining, and colorectal mucosa was for hematoxylin and eosin (H&E) staining, 16 S ribosomal RNA (rRNA) sequencing and immunohistochemistry for Major Histocompatibility Complex (MHC). Colon tissues and serum from caudal vein was collected for quantification of interleukin (IL)-6, IL-8, IL10 and tumor necrosis factor (TNF-α). Results Mice with HP CagA+ infection developed loss of some resident cells and inflammation infiltration in colorectal mucosa, and increased Giemsa-positive cells in gastric tissue. Also, MHC II-positive cells were increased in colorectal tissue in HP CagA+ strains infection. HP CagA+ infection cause increase of TNF-α, IL-6, IL-8 and IL-10 in the serum. Meanwhile, HP CagA+ stainis evoked gut microbiota dysbiosis which was characterized by altered microbiome distribution, reduction in Front-to-Back (F/B ratio), decreased α-diversity metric (Chao1 and Shannon). In β-diversity, gut microbiota in control and HP CagA+ groups showed the significant distance based on UniFrac distance. Cag group was enriched a higher abundance of Staphylococcus and Corynebacterium, while control subjects were enriched in Marinifilaceae and Odoribacter. Conclusion HP CagA+ strains are capable of causing gut microbiota dysbiosis to develop destruction of intestinal barrier, and it may affect the development of colorectal cancer by increasing colonization of Staphylococcus and Corynebacterium.https://doi.org/10.1186/s12876-025-03631-6CagA+Colorectal lesionGut microbiotaHelicobacter pylori |
| spellingShingle | Shasha Cui Xinqiang Liu Fengxia Han Lu Zhang Jingjing Bu Sainan Wu Jiafen Wang Helicobacter pylori CagA+ strains modulate colorectal pathology by regulating intestinal flora BMC Gastroenterology CagA+ Colorectal lesion Gut microbiota Helicobacter pylori |
| title | Helicobacter pylori CagA+ strains modulate colorectal pathology by regulating intestinal flora |
| title_full | Helicobacter pylori CagA+ strains modulate colorectal pathology by regulating intestinal flora |
| title_fullStr | Helicobacter pylori CagA+ strains modulate colorectal pathology by regulating intestinal flora |
| title_full_unstemmed | Helicobacter pylori CagA+ strains modulate colorectal pathology by regulating intestinal flora |
| title_short | Helicobacter pylori CagA+ strains modulate colorectal pathology by regulating intestinal flora |
| title_sort | helicobacter pylori caga strains modulate colorectal pathology by regulating intestinal flora |
| topic | CagA+ Colorectal lesion Gut microbiota Helicobacter pylori |
| url | https://doi.org/10.1186/s12876-025-03631-6 |
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