An Optimized Protocol for Simultaneous Propagation of Patient-derived Organoids and Matching CAFs
Recurrent hormone receptor-positive (HR+) breast cancer is a leading cause of cancer mortality in women. Recurrence and resistance to targeted therapies have been difficult to study due to the long clinical course of the disease, the complex nature of resistance, and the lack of clinically relevant...
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Bio-protocol LLC
2025-01-01
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| Online Access: | https://bio-protocol.org/en/bpdetail?id=5160&type=0 |
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| author | Jenny Högström Taru Muranen |
| author_facet | Jenny Högström Taru Muranen |
| author_sort | Jenny Högström |
| collection | DOAJ |
| description | Recurrent hormone receptor-positive (HR+) breast cancer is a leading cause of cancer mortality in women. Recurrence and resistance to targeted therapies have been difficult to study due to the long clinical course of the disease, the complex nature of resistance, and the lack of clinically relevant model systems. Existing models are limited to a few HR+ cell lines, organoid models, and patient-derived xenograft models, all lacking components of the human tumor microenvironment. Furthermore, the low take rate and loss of estrogen receptor (ER) expression in patient-derived organoids (PDOs) has been challenging. Our protocol allows simultaneous isolation of PDOs and matching cancer-associated fibroblasts (CAFs) from primary and metastatic HR+ breast cancers. Importantly, our protocol has a higher take rate and enables long-term culturing of PDOs that retain ER expression. Our matching PDOs and CAFs will provide researchers with a new resource to study the influence of the tumor microenvironment on various aspects of cancer biology such as cell growth and drug resistance in HR+ breast cancer. |
| format | Article |
| id | doaj-art-67875fd09bab4666baada26aeee332cf |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-67875fd09bab4666baada26aeee332cf2025-02-07T08:16:38ZengBio-protocol LLCBio-Protocol2331-83252025-01-0115210.21769/BioProtoc.5160An Optimized Protocol for Simultaneous Propagation of Patient-derived Organoids and Matching CAFsJenny Högström0Taru Muranen1Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USADepartment of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USARecurrent hormone receptor-positive (HR+) breast cancer is a leading cause of cancer mortality in women. Recurrence and resistance to targeted therapies have been difficult to study due to the long clinical course of the disease, the complex nature of resistance, and the lack of clinically relevant model systems. Existing models are limited to a few HR+ cell lines, organoid models, and patient-derived xenograft models, all lacking components of the human tumor microenvironment. Furthermore, the low take rate and loss of estrogen receptor (ER) expression in patient-derived organoids (PDOs) has been challenging. Our protocol allows simultaneous isolation of PDOs and matching cancer-associated fibroblasts (CAFs) from primary and metastatic HR+ breast cancers. Importantly, our protocol has a higher take rate and enables long-term culturing of PDOs that retain ER expression. Our matching PDOs and CAFs will provide researchers with a new resource to study the influence of the tumor microenvironment on various aspects of cancer biology such as cell growth and drug resistance in HR+ breast cancer.https://bio-protocol.org/en/bpdetail?id=5160&type=0 |
| spellingShingle | Jenny Högström Taru Muranen An Optimized Protocol for Simultaneous Propagation of Patient-derived Organoids and Matching CAFs Bio-Protocol |
| title | An Optimized Protocol for Simultaneous Propagation of Patient-derived Organoids and Matching CAFs |
| title_full | An Optimized Protocol for Simultaneous Propagation of Patient-derived Organoids and Matching CAFs |
| title_fullStr | An Optimized Protocol for Simultaneous Propagation of Patient-derived Organoids and Matching CAFs |
| title_full_unstemmed | An Optimized Protocol for Simultaneous Propagation of Patient-derived Organoids and Matching CAFs |
| title_short | An Optimized Protocol for Simultaneous Propagation of Patient-derived Organoids and Matching CAFs |
| title_sort | optimized protocol for simultaneous propagation of patient derived organoids and matching cafs |
| url | https://bio-protocol.org/en/bpdetail?id=5160&type=0 |
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