Mitigation PFHxA-induced neurotoxicity in Carassius auratus brain cells by selenium-enriched Bacillus subtilis via the BDNF/PI3K/AKT/GSK-3β pathway
As emerging contaminants growing threat to aquatic organisms, explore effective mitigation strategies is particularly important. Our previous studies have shown that selenium-rich Bacillus subtilis can not only alleviate the cause of brain damage by perfluorohexanoic acid (PFHxA) in Carassius auratu...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324016439 |
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| author | Yu-ting Lu Zheng-yao Guo Lei Guo Yu-hua He Li-ming Liu Xue Jiao Yue-hong Li |
| author_facet | Yu-ting Lu Zheng-yao Guo Lei Guo Yu-hua He Li-ming Liu Xue Jiao Yue-hong Li |
| author_sort | Yu-ting Lu |
| collection | DOAJ |
| description | As emerging contaminants growing threat to aquatic organisms, explore effective mitigation strategies is particularly important. Our previous studies have shown that selenium-rich Bacillus subtilis can not only alleviate the cause of brain damage by perfluorohexanoic acid (PFHxA) in Carassius auratus via the intestinal axis of the brain, but its metabolites can also alleviate PFHxA toxicity. This study further explores the potential mechanism through in vitro experiments. Findings demonstrate that apoptosis caused by PFHXA is effectively reduced with the use of selenium-rich Bacillus subtilis, which operates through the BDNF/PI3K/AKT/GSK-3β signalling pathway. Furthermore, utilisation of LY294002 and LICl inhibitors provided additional confirmation of the pivotal function of this pathway in neuroprotection. Our study results emphasize the significance of the PI3K/AKT/GSK-3β signalling pathway in promoting neuronal survival. Additionally, our findings establish a novel theoretical framework for using selenium-enriched Bacillus subtilis in environmental toxicology. Selenium-enriched Bacillus subtilis can be used as a novel microecological preparation. Implementing this approach could effectively counteract neurotoxic consequences of emerging contaminants, hence safeguarding and preserving aquatic ecosystems. |
| format | Article |
| id | doaj-art-67fd9ed170d94f8d92c4f89ecf686629 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-67fd9ed170d94f8d92c4f89ecf6866292025-02-12T05:29:48ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117567Mitigation PFHxA-induced neurotoxicity in Carassius auratus brain cells by selenium-enriched Bacillus subtilis via the BDNF/PI3K/AKT/GSK-3β pathwayYu-ting Lu0Zheng-yao Guo1Lei Guo2Yu-hua He3Li-ming Liu4Xue Jiao5Yue-hong Li6Jilin Agricultural Science and Technology University, Animal science and Technology College, Jilin, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaChangchun University of Chinese Medicine Affiliated Hospital, Changchun, ChinaJilin Agricultural Science and Technology University, Animal science and Technology College, Jilin, ChinaJilin Agricultural Science and Technology University, Animal science and Technology College, Jilin, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, China; Corresponding authors.College of Animal Science and Technology, Jilin Agricultural University, Changchun, China; Corresponding authors.As emerging contaminants growing threat to aquatic organisms, explore effective mitigation strategies is particularly important. Our previous studies have shown that selenium-rich Bacillus subtilis can not only alleviate the cause of brain damage by perfluorohexanoic acid (PFHxA) in Carassius auratus via the intestinal axis of the brain, but its metabolites can also alleviate PFHxA toxicity. This study further explores the potential mechanism through in vitro experiments. Findings demonstrate that apoptosis caused by PFHXA is effectively reduced with the use of selenium-rich Bacillus subtilis, which operates through the BDNF/PI3K/AKT/GSK-3β signalling pathway. Furthermore, utilisation of LY294002 and LICl inhibitors provided additional confirmation of the pivotal function of this pathway in neuroprotection. Our study results emphasize the significance of the PI3K/AKT/GSK-3β signalling pathway in promoting neuronal survival. Additionally, our findings establish a novel theoretical framework for using selenium-enriched Bacillus subtilis in environmental toxicology. Selenium-enriched Bacillus subtilis can be used as a novel microecological preparation. Implementing this approach could effectively counteract neurotoxic consequences of emerging contaminants, hence safeguarding and preserving aquatic ecosystems.http://www.sciencedirect.com/science/article/pii/S0147651324016439Selenium-enriched Bacillus subtilisPerfluorohexanoic acid (PFHxA)NeurotoxicityBDNF/PI3K/AKT/GSK-3β signaling pathwayApoptosis |
| spellingShingle | Yu-ting Lu Zheng-yao Guo Lei Guo Yu-hua He Li-ming Liu Xue Jiao Yue-hong Li Mitigation PFHxA-induced neurotoxicity in Carassius auratus brain cells by selenium-enriched Bacillus subtilis via the BDNF/PI3K/AKT/GSK-3β pathway Ecotoxicology and Environmental Safety Selenium-enriched Bacillus subtilis Perfluorohexanoic acid (PFHxA) Neurotoxicity BDNF/PI3K/AKT/GSK-3β signaling pathway Apoptosis |
| title | Mitigation PFHxA-induced neurotoxicity in Carassius auratus brain cells by selenium-enriched Bacillus subtilis via the BDNF/PI3K/AKT/GSK-3β pathway |
| title_full | Mitigation PFHxA-induced neurotoxicity in Carassius auratus brain cells by selenium-enriched Bacillus subtilis via the BDNF/PI3K/AKT/GSK-3β pathway |
| title_fullStr | Mitigation PFHxA-induced neurotoxicity in Carassius auratus brain cells by selenium-enriched Bacillus subtilis via the BDNF/PI3K/AKT/GSK-3β pathway |
| title_full_unstemmed | Mitigation PFHxA-induced neurotoxicity in Carassius auratus brain cells by selenium-enriched Bacillus subtilis via the BDNF/PI3K/AKT/GSK-3β pathway |
| title_short | Mitigation PFHxA-induced neurotoxicity in Carassius auratus brain cells by selenium-enriched Bacillus subtilis via the BDNF/PI3K/AKT/GSK-3β pathway |
| title_sort | mitigation pfhxa induced neurotoxicity in carassius auratus brain cells by selenium enriched bacillus subtilis via the bdnf pi3k akt gsk 3β pathway |
| topic | Selenium-enriched Bacillus subtilis Perfluorohexanoic acid (PFHxA) Neurotoxicity BDNF/PI3K/AKT/GSK-3β signaling pathway Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324016439 |
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