EVALUATION OF POLYMORPHONUCLEAR IMMUNE CELLS IN BIOCOMPATIBILITY TEST DFLP SPONGE CARTILAGE SCAFFOLD, ADIPOSE-DERIVED MSC AND SECRETOME WITH CARTILAGE INJECTION MODEL
Background: In recent years, Freeze-Dried Scaffold Bovine Cartilage has been widely used as an alternative therapy for joint cartilage defects. This study aims to determine the biocompatibility of scaffold without involving implantation which provides clinical reports as expected through the evaluat...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Airlangga
2020-04-01
|
| Series: | Journal Orthopaedi and Traumatology Surabaya |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/JOINTS/article/view/17954 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856500677804032 |
|---|---|
| author | Brilliant Citra Wirashada Dwikora Novemberi Utomo Lukas Widhiyanto |
| author_facet | Brilliant Citra Wirashada Dwikora Novemberi Utomo Lukas Widhiyanto |
| author_sort | Brilliant Citra Wirashada |
| collection | DOAJ |
| description | Background: In recent years, Freeze-Dried Scaffold Bovine Cartilage has been widely used as an alternative therapy for joint cartilage defects. This study aims to determine the biocompatibility of scaffold without involving implantation which provides clinical reports as expected through the evaluation of post-implantation chondrocytes regeneration, biocompatibility markers of the scaffold, and biocompatibility of sponge cartilage scaffold involving cartilage defects New Zealand White Rabbit.
Methods: This experimental in-vivo study was conducted for four weeks. Rabbits were divided into 4 treatment groups: microfracture defect group with DFLP sponge cartilage scaffold (P1) implantation; Microfracture defect group with DFLP sponge cartilage scaffold-secretome implantation (P2); Microfracture defect group with DFLP sponge cartilage scaffold-adipose derived Mesenchymal Stem Cells (ADMSCs) (P3); Microfracture defect group without implantation (control). The evaluations of basophil, eosinophil, neutrophil, and polymorphonuclear (PMN) cells were done in the first 24 hours, 3 days, and 1 week after the treatment. The collected data will be analyzed statistically.
Results: Research observations performed three times in the first, third, and seventh days. The results showed a small number of average Neutrophil (Neutrophil granulated) and PMN (segmented Neutrophils) cells both in the P2 and P3 groups compared with the control and the P1 group.
Conclusion: In general, biocompatibility is not included on the cytotoxic effects including inflammatory reactions and post-cartilage scaffold sponge implantation (DFLP) with or without the addition of ADMSC and secretome in the white rabbit New Zealand cartilage defect associated with differences seen in eosinophils, basophils, neutrophils, also total PMN cells in four groups. |
| format | Article |
| id | doaj-art-682a24b5301b4c8ca333e319b37c9c98 |
| institution | Kabale University |
| issn | 2722-712X 2460-8742 |
| language | English |
| publishDate | 2020-04-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal Orthopaedi and Traumatology Surabaya |
| spelling | doaj-art-682a24b5301b4c8ca333e319b37c9c982025-02-12T07:28:50ZengUniversitas AirlanggaJournal Orthopaedi and Traumatology Surabaya2722-712X2460-87422020-04-01911810.20473/joints.v9i1.2020.1-814668EVALUATION OF POLYMORPHONUCLEAR IMMUNE CELLS IN BIOCOMPATIBILITY TEST DFLP SPONGE CARTILAGE SCAFFOLD, ADIPOSE-DERIVED MSC AND SECRETOME WITH CARTILAGE INJECTION MODELBrilliant Citra Wirashada0Dwikora Novemberi Utomo1https://orcid.org/0000-0002-7832-5695Lukas Widhiyanto2https://orcid.org/0000-0002-1241-6172Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Airlangga/ Dr. Soetomo General Hospital, SurabayaDepartment of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Airlangga/ Dr. Soetomo General Hospital, SurabayaDepartment of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Airlangga/ Dr. Soetomo General Hospital, SurabayaBackground: In recent years, Freeze-Dried Scaffold Bovine Cartilage has been widely used as an alternative therapy for joint cartilage defects. This study aims to determine the biocompatibility of scaffold without involving implantation which provides clinical reports as expected through the evaluation of post-implantation chondrocytes regeneration, biocompatibility markers of the scaffold, and biocompatibility of sponge cartilage scaffold involving cartilage defects New Zealand White Rabbit. Methods: This experimental in-vivo study was conducted for four weeks. Rabbits were divided into 4 treatment groups: microfracture defect group with DFLP sponge cartilage scaffold (P1) implantation; Microfracture defect group with DFLP sponge cartilage scaffold-secretome implantation (P2); Microfracture defect group with DFLP sponge cartilage scaffold-adipose derived Mesenchymal Stem Cells (ADMSCs) (P3); Microfracture defect group without implantation (control). The evaluations of basophil, eosinophil, neutrophil, and polymorphonuclear (PMN) cells were done in the first 24 hours, 3 days, and 1 week after the treatment. The collected data will be analyzed statistically. Results: Research observations performed three times in the first, third, and seventh days. The results showed a small number of average Neutrophil (Neutrophil granulated) and PMN (segmented Neutrophils) cells both in the P2 and P3 groups compared with the control and the P1 group. Conclusion: In general, biocompatibility is not included on the cytotoxic effects including inflammatory reactions and post-cartilage scaffold sponge implantation (DFLP) with or without the addition of ADMSC and secretome in the white rabbit New Zealand cartilage defect associated with differences seen in eosinophils, basophils, neutrophils, also total PMN cells in four groups.https://e-journal.unair.ac.id/JOINTS/article/view/17954scaffoldcartilage jointadmscssecretomehuman and medicine |
| spellingShingle | Brilliant Citra Wirashada Dwikora Novemberi Utomo Lukas Widhiyanto EVALUATION OF POLYMORPHONUCLEAR IMMUNE CELLS IN BIOCOMPATIBILITY TEST DFLP SPONGE CARTILAGE SCAFFOLD, ADIPOSE-DERIVED MSC AND SECRETOME WITH CARTILAGE INJECTION MODEL Journal Orthopaedi and Traumatology Surabaya scaffold cartilage joint admscs secretome human and medicine |
| title | EVALUATION OF POLYMORPHONUCLEAR IMMUNE CELLS IN BIOCOMPATIBILITY TEST DFLP SPONGE CARTILAGE SCAFFOLD, ADIPOSE-DERIVED MSC AND SECRETOME WITH CARTILAGE INJECTION MODEL |
| title_full | EVALUATION OF POLYMORPHONUCLEAR IMMUNE CELLS IN BIOCOMPATIBILITY TEST DFLP SPONGE CARTILAGE SCAFFOLD, ADIPOSE-DERIVED MSC AND SECRETOME WITH CARTILAGE INJECTION MODEL |
| title_fullStr | EVALUATION OF POLYMORPHONUCLEAR IMMUNE CELLS IN BIOCOMPATIBILITY TEST DFLP SPONGE CARTILAGE SCAFFOLD, ADIPOSE-DERIVED MSC AND SECRETOME WITH CARTILAGE INJECTION MODEL |
| title_full_unstemmed | EVALUATION OF POLYMORPHONUCLEAR IMMUNE CELLS IN BIOCOMPATIBILITY TEST DFLP SPONGE CARTILAGE SCAFFOLD, ADIPOSE-DERIVED MSC AND SECRETOME WITH CARTILAGE INJECTION MODEL |
| title_short | EVALUATION OF POLYMORPHONUCLEAR IMMUNE CELLS IN BIOCOMPATIBILITY TEST DFLP SPONGE CARTILAGE SCAFFOLD, ADIPOSE-DERIVED MSC AND SECRETOME WITH CARTILAGE INJECTION MODEL |
| title_sort | evaluation of polymorphonuclear immune cells in biocompatibility test dflp sponge cartilage scaffold adipose derived msc and secretome with cartilage injection model |
| topic | scaffold cartilage joint admscs secretome human and medicine |
| url | https://e-journal.unair.ac.id/JOINTS/article/view/17954 |
| work_keys_str_mv | AT brilliantcitrawirashada evaluationofpolymorphonuclearimmunecellsinbiocompatibilitytestdflpspongecartilagescaffoldadiposederivedmscandsecretomewithcartilageinjectionmodel AT dwikoranovemberiutomo evaluationofpolymorphonuclearimmunecellsinbiocompatibilitytestdflpspongecartilagescaffoldadiposederivedmscandsecretomewithcartilageinjectionmodel AT lukaswidhiyanto evaluationofpolymorphonuclearimmunecellsinbiocompatibilitytestdflpspongecartilagescaffoldadiposederivedmscandsecretomewithcartilageinjectionmodel |