Identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound-specific isotope analysis
Extracellular polymeric substances (EPS) refer to a wide variety of high molecular weight molecules secreted outside the cell membrane by biofilm microorganisms. In the present study, EPS from marine microphytobenthic biofilms were extracted and their isotope ratios were analysed. A comparison of th...
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2023-10-01
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| Online Access: | https://peercommunityjournal.org/articles/10.24072/pcjournal.336/ |
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| author | Hubas, Cédric Gaubert-Boussarie, Julie D’Hondt, An-Sofie Jesus, Bruno Lamy, Dominique Meleder, Vona Prins, Antoine Rosa, Philippe Stock, Willem Sabbe, Koen |
| author_facet | Hubas, Cédric Gaubert-Boussarie, Julie D’Hondt, An-Sofie Jesus, Bruno Lamy, Dominique Meleder, Vona Prins, Antoine Rosa, Philippe Stock, Willem Sabbe, Koen |
| author_sort | Hubas, Cédric |
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| description | Extracellular polymeric substances (EPS) refer to a wide variety of high molecular weight molecules secreted outside the cell membrane by biofilm microorganisms. In the present study, EPS from marine microphytobenthic biofilms were extracted and their isotope ratios were analysed. A comparison of these ratios with the carbon isotope ratios of fatty acid biomarkers allowed the identification of the main EPS producers of two contrasting types of intertidal marine sediments. Our study reveals that EPS sources are more diverse in sandy sediments than in muddy sediments. We also found distinct patterns in the production and breakdown of EPS in sandy and muddy environments. The main difference observed was in how epipelic and epipsammic diatoms affected the chemistry of EPS, which had significant implications for the growth of bacteria specialized in utilizing EPS. These differences were likely linked to variations in the functioning of epipelic and epipsammic communities, specifically in how EPS was used either for motility or for cell attachment.
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| format | Article |
| id | doaj-art-68865a1f96ed4086a890720e605da9d0 |
| institution | Kabale University |
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| language | English |
| publishDate | 2023-10-01 |
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| series | Peer Community Journal |
| spelling | doaj-art-68865a1f96ed4086a890720e605da9d02025-02-07T10:16:48ZengPeer Community InPeer Community Journal2804-38712023-10-01310.24072/pcjournal.33610.24072/pcjournal.336Identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound-specific isotope analysis Hubas, Cédric0https://orcid.org/0000-0002-9110-9292Gaubert-Boussarie, Julie1D’Hondt, An-Sofie2https://orcid.org/0000-0002-4275-5773Jesus, Bruno3https://orcid.org/0000-0002-2047-3783Lamy, Dominique4Meleder, Vona5https://orcid.org/0000-0002-1874-1929Prins, Antoine6Rosa, Philippe7https://orcid.org/0000-0001-6621-3445Stock, Willem8https://orcid.org/0000-0001-7648-6404Sabbe, Koen9https://orcid.org/0000-0001-5163-5581Laboratoire de Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Muséum National d’Histoire Naturelle, CNRS, Sorbonne Université, IRD, Université des Antilles, Université de Caen Normandie; Station Marine de Concarneau, Place de la croix,29900, Concarneau, FranceLaboratoire de Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Muséum National d’Histoire Naturelle, CNRS, Sorbonne Université, IRD, Université des Antilles, Université de Caen Normandie; Station Marine de Concarneau, Place de la croix,29900, Concarneau, FranceDepartment of Biology, Marine Biology Research Group, Ghent University, Krijgslaan 281/S8, 9000, BelgiumNantes Université, Institut des Substances et Organismes de la Mer, ISOMer, UR 2160, F-44000 Nantes, FranceLaboratoire de Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Muséum National d’Histoire Naturelle, CNRS, Sorbonne Université, IRD, Université des Antilles, Université de Caen Normandie; Jardin des plantes, Bâtiment arthropodes, Paris, France; Institute of Ecology and Environmental Sciences of Paris (iEES-Paris), Sorbonne Université, Univ Paris Est Créteil, IRD, CNRS, INRA, 4 place Jussieu, 75005 Paris, FranceNantes Université, Institut des Substances et Organismes de la Mer, ISOMer, UR 2160, F-44000 Nantes, FranceLaboratoire de Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Muséum National d’Histoire Naturelle, CNRS, Sorbonne Université, IRD, Université des Antilles, Université de Caen Normandie; Station Marine de Concarneau, Place de la croix,29900, Concarneau, France; Nantes Université, Institut des Substances et Organismes de la Mer, ISOMer, UR 2160, F-44000 Nantes, FranceNantes Université, Institut des Substances et Organismes de la Mer, ISOMer, UR 2160, F-44000 Nantes, FranceDepartment of Biology, Research Group Protistology and Aquatic Ecology, Ghent University, Krijgslaan 281/S8, 9000, BelgiumDepartment of Biology, Research Group Protistology and Aquatic Ecology, Ghent University, Krijgslaan 281/S8, 9000, BelgiumExtracellular polymeric substances (EPS) refer to a wide variety of high molecular weight molecules secreted outside the cell membrane by biofilm microorganisms. In the present study, EPS from marine microphytobenthic biofilms were extracted and their isotope ratios were analysed. A comparison of these ratios with the carbon isotope ratios of fatty acid biomarkers allowed the identification of the main EPS producers of two contrasting types of intertidal marine sediments. Our study reveals that EPS sources are more diverse in sandy sediments than in muddy sediments. We also found distinct patterns in the production and breakdown of EPS in sandy and muddy environments. The main difference observed was in how epipelic and epipsammic diatoms affected the chemistry of EPS, which had significant implications for the growth of bacteria specialized in utilizing EPS. These differences were likely linked to variations in the functioning of epipelic and epipsammic communities, specifically in how EPS was used either for motility or for cell attachment. https://peercommunityjournal.org/articles/10.24072/pcjournal.336/Extracellular Polymeric SubstancesStable isotopescompound specific isotope analysisfatty acids |
| spellingShingle | Hubas, Cédric Gaubert-Boussarie, Julie D’Hondt, An-Sofie Jesus, Bruno Lamy, Dominique Meleder, Vona Prins, Antoine Rosa, Philippe Stock, Willem Sabbe, Koen Identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound-specific isotope analysis Peer Community Journal Extracellular Polymeric Substances Stable isotopes compound specific isotope analysis fatty acids |
| title | Identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound-specific isotope analysis
|
| title_full | Identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound-specific isotope analysis
|
| title_fullStr | Identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound-specific isotope analysis
|
| title_full_unstemmed | Identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound-specific isotope analysis
|
| title_short | Identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound-specific isotope analysis
|
| title_sort | identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound specific isotope analysis |
| topic | Extracellular Polymeric Substances Stable isotopes compound specific isotope analysis fatty acids |
| url | https://peercommunityjournal.org/articles/10.24072/pcjournal.336/ |
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