Identification of a new fish trypanosome from the large yellow croaker (Larimichthys crocea) and description of its impact on host pathology, blood biochemical parameters and immune responses
The aim of this study was to clarify the taxonomic identification of a hemoflagellate and assess the effect of trypanosome infection on Larimichthys crocea. Giemsa staining showed the presence of three morphotypes of trypomastigotes. The trypanosomes had the following morphological characteristics:...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2025-01-01
|
| Series: | Parasite |
| Subjects: | |
| Online Access: | https://www.parasite-journal.org/articles/parasite/full_html/2025/01/parasite240150/parasite240150.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825206566957088768 |
|---|---|
| author | Yang Xiaoao Qi Pengzhi Tao Zhen Zhang Qingwei Wang Yanjie Zhu Denghui Yan Xiaojun Fu Peipei Guo Baoying |
| author_facet | Yang Xiaoao Qi Pengzhi Tao Zhen Zhang Qingwei Wang Yanjie Zhu Denghui Yan Xiaojun Fu Peipei Guo Baoying |
| author_sort | Yang Xiaoao |
| collection | DOAJ |
| description | The aim of this study was to clarify the taxonomic identification of a hemoflagellate and assess the effect of trypanosome infection on Larimichthys crocea. Giemsa staining showed the presence of three morphotypes of trypomastigotes. The trypanosomes had the following morphological characteristics: a slender body with a long flagellum at the front; body size 12.30–30.90 × 1.13–2.33 μm; elongated oval nucleus situated in the median region; kinetoplast small, oval, located at the posterior end. The parasite had significant morphological differences from Trypanosoma epinepheli Su, Feng, Jiang, Guo, Liu & Xu, 2014 and Trypanosoma carassii (Mitrofanov) Doflein, 1901. The 18S rDNA sequences of the trypanosome from L. crocea had the highest homology (98.4%) with T. carassii. Phylogenetic analysis indicated that the parasite clustered with freshwater fish trypanosomes. Based on the differences in morphological characteristics and molecular data, it is considered a new species, Trypanosoma larimichthysi n. sp. Trypanosome infection had no effect on the growth of L. crocea, but significantly increased the concentration of blood urea nitrogen (BUN), and induced pathological changes in the gills, liver, spleen and kidney. The pro-inflammatory immune genes, including TNF-α, IFN-γ, IL-1β, CXCL8 and iNOS, were significantly upregulated in the L. crocea infected with trypanosomes. These results suggest that the trypanosome has negative impacts on host health. |
| format | Article |
| id | doaj-art-6979dbe53ffa4eb2b64a7831b804e3a9 |
| institution | Kabale University |
| issn | 1776-1042 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj-art-6979dbe53ffa4eb2b64a7831b804e3a92025-02-07T08:26:35ZengEDP SciencesParasite1776-10422025-01-0132110.1051/parasite/2024078parasite240150Identification of a new fish trypanosome from the large yellow croaker (Larimichthys crocea) and description of its impact on host pathology, blood biochemical parameters and immune responsesYang Xiaoao0Qi Pengzhi1Tao Zhen2Zhang Qingwei3Wang Yanjie4Zhu Denghui5Yan Xiaojun6Fu Peipeihttps://orcid.org/0000-0003-1644-2279Guo BaoyingNational Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean UniversityNational Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean UniversitySchool of Fisheries, Zhejiang Ocean UniversitySchool of Fisheries, Zhejiang Ocean UniversityNational Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean UniversityTaizhou Key Laboratory of Biomedicine and Advanced Dosage Forms, School of Life Sciences, Taizhou UniversityNational Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean UniversityThe aim of this study was to clarify the taxonomic identification of a hemoflagellate and assess the effect of trypanosome infection on Larimichthys crocea. Giemsa staining showed the presence of three morphotypes of trypomastigotes. The trypanosomes had the following morphological characteristics: a slender body with a long flagellum at the front; body size 12.30–30.90 × 1.13–2.33 μm; elongated oval nucleus situated in the median region; kinetoplast small, oval, located at the posterior end. The parasite had significant morphological differences from Trypanosoma epinepheli Su, Feng, Jiang, Guo, Liu & Xu, 2014 and Trypanosoma carassii (Mitrofanov) Doflein, 1901. The 18S rDNA sequences of the trypanosome from L. crocea had the highest homology (98.4%) with T. carassii. Phylogenetic analysis indicated that the parasite clustered with freshwater fish trypanosomes. Based on the differences in morphological characteristics and molecular data, it is considered a new species, Trypanosoma larimichthysi n. sp. Trypanosome infection had no effect on the growth of L. crocea, but significantly increased the concentration of blood urea nitrogen (BUN), and induced pathological changes in the gills, liver, spleen and kidney. The pro-inflammatory immune genes, including TNF-α, IFN-γ, IL-1β, CXCL8 and iNOS, were significantly upregulated in the L. crocea infected with trypanosomes. These results suggest that the trypanosome has negative impacts on host health.https://www.parasite-journal.org/articles/parasite/full_html/2025/01/parasite240150/parasite240150.htmltrypanosomalarimichthys croceaidentificationpathologyimmunity |
| spellingShingle | Yang Xiaoao Qi Pengzhi Tao Zhen Zhang Qingwei Wang Yanjie Zhu Denghui Yan Xiaojun Fu Peipei Guo Baoying Identification of a new fish trypanosome from the large yellow croaker (Larimichthys crocea) and description of its impact on host pathology, blood biochemical parameters and immune responses Parasite trypanosoma larimichthys crocea identification pathology immunity |
| title | Identification of a new fish trypanosome from the large yellow croaker (Larimichthys crocea) and description of its impact on host pathology, blood biochemical parameters and immune responses |
| title_full | Identification of a new fish trypanosome from the large yellow croaker (Larimichthys crocea) and description of its impact on host pathology, blood biochemical parameters and immune responses |
| title_fullStr | Identification of a new fish trypanosome from the large yellow croaker (Larimichthys crocea) and description of its impact on host pathology, blood biochemical parameters and immune responses |
| title_full_unstemmed | Identification of a new fish trypanosome from the large yellow croaker (Larimichthys crocea) and description of its impact on host pathology, blood biochemical parameters and immune responses |
| title_short | Identification of a new fish trypanosome from the large yellow croaker (Larimichthys crocea) and description of its impact on host pathology, blood biochemical parameters and immune responses |
| title_sort | identification of a new fish trypanosome from the large yellow croaker larimichthys crocea and description of its impact on host pathology blood biochemical parameters and immune responses |
| topic | trypanosoma larimichthys crocea identification pathology immunity |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2025/01/parasite240150/parasite240150.html |
| work_keys_str_mv | AT yangxiaoao identificationofanewfishtrypanosomefromthelargeyellowcroakerlarimichthyscroceaanddescriptionofitsimpactonhostpathologybloodbiochemicalparametersandimmuneresponses AT qipengzhi identificationofanewfishtrypanosomefromthelargeyellowcroakerlarimichthyscroceaanddescriptionofitsimpactonhostpathologybloodbiochemicalparametersandimmuneresponses AT taozhen identificationofanewfishtrypanosomefromthelargeyellowcroakerlarimichthyscroceaanddescriptionofitsimpactonhostpathologybloodbiochemicalparametersandimmuneresponses AT zhangqingwei identificationofanewfishtrypanosomefromthelargeyellowcroakerlarimichthyscroceaanddescriptionofitsimpactonhostpathologybloodbiochemicalparametersandimmuneresponses AT wangyanjie identificationofanewfishtrypanosomefromthelargeyellowcroakerlarimichthyscroceaanddescriptionofitsimpactonhostpathologybloodbiochemicalparametersandimmuneresponses AT zhudenghui identificationofanewfishtrypanosomefromthelargeyellowcroakerlarimichthyscroceaanddescriptionofitsimpactonhostpathologybloodbiochemicalparametersandimmuneresponses AT yanxiaojun identificationofanewfishtrypanosomefromthelargeyellowcroakerlarimichthyscroceaanddescriptionofitsimpactonhostpathologybloodbiochemicalparametersandimmuneresponses AT fupeipei identificationofanewfishtrypanosomefromthelargeyellowcroakerlarimichthyscroceaanddescriptionofitsimpactonhostpathologybloodbiochemicalparametersandimmuneresponses AT guobaoying identificationofanewfishtrypanosomefromthelargeyellowcroakerlarimichthyscroceaanddescriptionofitsimpactonhostpathologybloodbiochemicalparametersandimmuneresponses |