Investigating the mechanistic role of oxidative stress in brain–gut axis disruption in rat model
This study investigated the impact of endotoxin exposure on intestinal oxidative stress and white blood cell (WBC) subtypes in a rat model to understand its role in disrupting brain–gut communication. Lipopolysaccharide (LPS)-derived endotoxin was intraperitoneally injected into Sprague D...
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Academia.edu Journals
2024-11-01
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| Series: | Academia Biology |
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| author | Akeem Babatunde Sikiru Stephen Acheneje Egena Muhyideen Adio Kilani Morufat Eniola Azeez Maryam Nahari Adam Ahmad Abdullahi |
| author_facet | Akeem Babatunde Sikiru Stephen Acheneje Egena Muhyideen Adio Kilani Morufat Eniola Azeez Maryam Nahari Adam Ahmad Abdullahi |
| author_sort | Akeem Babatunde Sikiru |
| collection | DOAJ |
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This study investigated the impact of endotoxin exposure on intestinal oxidative stress and white blood cell (WBC) subtypes in a rat model to understand its role in disrupting brain–gut communication. Lipopolysaccharide (LPS)-derived endotoxin was intraperitoneally injected into Sprague Dawley rats at varying doses of 250, 500, 750, and 1,000 μg per kg body weight four times weekly. The intestinal oxidative stress markers (superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), malondialdehyde (MDA)) and WBC differentials (WBCs, lymphocytes, monocytes, neutrophils) were measured. The endotoxin exposure significantly increased intestinal oxidative stress by decreasing the levels of SOD, CAT, and GSH, and it increased the level of MDA compared with the control group (p < 0.05). The endotoxin exposure caused nonspecific inflammatory response marked with increased counts of WBCs and neutrophils (p < 0.05). The monocyte percentage decreased (p < 0.05), while the lymphocyte percentage remained unchanged (p > 0.05). These findings suggest that endotoxin disrupts the gut’s antioxidant system and triggers inflammation, which could potentially lead to gut dysfunction. The findings also suggest a link between gut dysfunction and brain function, which implies a possible indirect effect of endotoxin on body reserve and reproduction via the brain–gut axis compromise. The study concluded that an understanding of oxidative stress and immune compromise, as reflected by changes in intestinal oxidative stress biomarkers and WBC subtypes, offers potential targets for therapeutic development against various diseases and performance issues related to the brain–gut axis. |
| format | Article |
| id | doaj-art-6999d74dfc2e4a698e9ebc9142a149ae |
| institution | Kabale University |
| issn | 2837-4010 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Academia.edu Journals |
| record_format | Article |
| series | Academia Biology |
| spelling | doaj-art-6999d74dfc2e4a698e9ebc9142a149ae2025-02-11T00:46:05ZengAcademia.edu JournalsAcademia Biology2837-40102024-11-012410.20935/AcadBiol7409Investigating the mechanistic role of oxidative stress in brain–gut axis disruption in rat modelAkeem Babatunde Sikiru0Stephen Acheneje Egena1Muhyideen Adio Kilani2Morufat Eniola Azeez3Maryam Nahari Adam4Ahmad Abdullahi5Department of Animal Science, Federal University of Agriculture, Zuru, Kebbi 872101, Nigeria.Department of Animal Production, Federal University of Technology, Minna, Niger 920101, Nigeria.Department of Veterinary Surgery and Radiology, Federal University of Agriculture, Zuru, Kebbi 872101, Nigeria.Department of Biology and Integrated Science, Emmanuel Alayande University of Education, Oyo 211101, Nigeria.Department of Biology, Federal University of Agriculture, Zuru, Kebbi 872101, Nigeria.Department of Animal Science, Federal University of Agriculture, Zuru, Kebbi 872101, Nigeria. This study investigated the impact of endotoxin exposure on intestinal oxidative stress and white blood cell (WBC) subtypes in a rat model to understand its role in disrupting brain–gut communication. Lipopolysaccharide (LPS)-derived endotoxin was intraperitoneally injected into Sprague Dawley rats at varying doses of 250, 500, 750, and 1,000 μg per kg body weight four times weekly. The intestinal oxidative stress markers (superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), malondialdehyde (MDA)) and WBC differentials (WBCs, lymphocytes, monocytes, neutrophils) were measured. The endotoxin exposure significantly increased intestinal oxidative stress by decreasing the levels of SOD, CAT, and GSH, and it increased the level of MDA compared with the control group (p < 0.05). The endotoxin exposure caused nonspecific inflammatory response marked with increased counts of WBCs and neutrophils (p < 0.05). The monocyte percentage decreased (p < 0.05), while the lymphocyte percentage remained unchanged (p > 0.05). These findings suggest that endotoxin disrupts the gut’s antioxidant system and triggers inflammation, which could potentially lead to gut dysfunction. The findings also suggest a link between gut dysfunction and brain function, which implies a possible indirect effect of endotoxin on body reserve and reproduction via the brain–gut axis compromise. The study concluded that an understanding of oxidative stress and immune compromise, as reflected by changes in intestinal oxidative stress biomarkers and WBC subtypes, offers potential targets for therapeutic development against various diseases and performance issues related to the brain–gut axis.https://www.academia.edu/125484322/Investigating_the_mechanistic_role_of_oxidative_stress_in_brain_gut_axis_disruption_in_rat_model |
| spellingShingle | Akeem Babatunde Sikiru Stephen Acheneje Egena Muhyideen Adio Kilani Morufat Eniola Azeez Maryam Nahari Adam Ahmad Abdullahi Investigating the mechanistic role of oxidative stress in brain–gut axis disruption in rat model Academia Biology |
| title | Investigating the mechanistic role of oxidative stress in brain–gut axis disruption in rat model |
| title_full | Investigating the mechanistic role of oxidative stress in brain–gut axis disruption in rat model |
| title_fullStr | Investigating the mechanistic role of oxidative stress in brain–gut axis disruption in rat model |
| title_full_unstemmed | Investigating the mechanistic role of oxidative stress in brain–gut axis disruption in rat model |
| title_short | Investigating the mechanistic role of oxidative stress in brain–gut axis disruption in rat model |
| title_sort | investigating the mechanistic role of oxidative stress in brain gut axis disruption in rat model |
| url | https://www.academia.edu/125484322/Investigating_the_mechanistic_role_of_oxidative_stress_in_brain_gut_axis_disruption_in_rat_model |
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