Antimicrobial susceptibilities of oral Neisseria from men on HIV pre-exposure prophylaxis in Hanoi, Vietnam
ABSTRACT: Background: Antimicrobial resistance (AMR) in Neisseria gonorrhoeae (N. gonorrhoeae) is an urgent global health concern. Commensal Neisseria species in the oropharynx are an important reservoir of AMR genes that are transferred to N. gonorrhoeae, yet few data about AMR among commensal Nei...
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Elsevier
2025-01-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524004491 |
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| author | Huan Vinh Dong Paul Adamson Dieu Hoa Pham Quynh Hoa Pham Hai Ha Long Le Loc Quang Pham Hao Thi Minh Bui Giang Minh Le Jeffrey D. Klausner |
| author_facet | Huan Vinh Dong Paul Adamson Dieu Hoa Pham Quynh Hoa Pham Hai Ha Long Le Loc Quang Pham Hao Thi Minh Bui Giang Minh Le Jeffrey D. Klausner |
| author_sort | Huan Vinh Dong |
| collection | DOAJ |
| description | ABSTRACT: Background: Antimicrobial resistance (AMR) in Neisseria gonorrhoeae (N. gonorrhoeae) is an urgent global health concern. Commensal Neisseria species in the oropharynx are an important reservoir of AMR genes that are transferred to N. gonorrhoeae, yet few data about AMR among commensal Neisseria in populations at risk for AMR exist. Methods: From May 2022 to December 2023, men in an HIV pre-exposure prophylaxis program in Hanoi, Vietnam, were recruited for this study. Participants self-collected oral specimens using phosphate buffer solution, for culture on LB agar media containing sucrose, vancomycin, and trimethoprim (LBVT.SNR). Oxidase-positive Gram-negative diplococci were identified using the Remel RapID NH system. Minimum inhibitory concentrations (MICs) to azithromycin, ceftriaxone, cefixime, and doxycycline were determined using Etests. Results: There were 42 male participants, the median age was 26 years and 29% (n = 12) reported using antibiotics in the past 6 months. In total, 48 Neisseria isolates were recovered; N. sicca/subflava was the most common species (50%; n = 24), followed by N. mucosa (38%; n = 18). For azithromycin, 85% (n = 41) of isolates were resistant with MICs ≥ 1 ug/mL, including 25% (n = 12) with high-level resistance (MICs ≥ 256 ug/mL of which 67% (8/12) were N. mucosa. Among non-gonococcal Neisseria isolates, the prevalence of resistance was 6% (n = 3) for ceftriaxone, 6% (n = 3) for cefixime, and 54% (n = 26) for doxycycline; the most non-susceptible isolates were N. mucosa. Conclusions: A High frequency of azithromycin resistance, moderate doxycycline resistance, and low cephalosporin resistance was found in oropharyngeal Neisseria isolates from men who have sex with men (MSM) in a pre-exposure prophylaxis (PrEP) program in Hanoi, Vietnam. N. mucosa was over-represented among resistant isolates. |
| format | Article |
| id | doaj-art-6b0af7e0817d48919775e073e8279128 |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-6b0af7e0817d48919775e073e82791282025-02-08T05:00:25ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-01-01401114Antimicrobial susceptibilities of oral Neisseria from men on HIV pre-exposure prophylaxis in Hanoi, VietnamHuan Vinh Dong0Paul Adamson1Dieu Hoa Pham2Quynh Hoa Pham3Hai Ha Long Le4Loc Quang Pham5Hao Thi Minh Bui6Giang Minh Le7Jeffrey D. Klausner8Division of Pediatric Infectious Diseases, David Geffen School of Medicine at University of California, Los Angeles (UCLA), Los Angeles, CA, USA; Corresponding author. Mailing address: David Geffen School of Medicine at UCLA, Department of Pediatrics/Peds Infectious Diseases, 10833 Le Conte Avenue, Room #12-311 MDCC, Mailcode: 175217, Los Angeles, CA 90095.Division of Infectious Diseases, David Geffen School of Medicine at UCLA, Los Angeles, CA, USANational Hospital for Dermatology and Venereology, Hanoi, VietnamNational Hospital for Dermatology and Venereology, Hanoi, VietnamNational Hospital for Dermatology and Venereology, Hanoi, Vietnam; Faculty of Medical Technology, Hanoi Medical University, Hanoi, VietnamCenter for Training and Research on Substance Abuse-HIV, Hanoi Medical University, Hanoi, Vietnam; School of Preventive Medicine and Public Health, Hanoi Medical University, Hanoi, VietnamCenter for Training and Research on Substance Abuse-HIV, Hanoi Medical University, Hanoi, VietnamCenter for Training and Research on Substance Abuse-HIV, Hanoi Medical University, Hanoi, Vietnam; School of Preventive Medicine and Public Health, Hanoi Medical University, Hanoi, VietnamDepartment of Population and Public Health Sciences, Keck School of Medicine, University of Southern California (USC), Los Angeles, CA, USAABSTRACT: Background: Antimicrobial resistance (AMR) in Neisseria gonorrhoeae (N. gonorrhoeae) is an urgent global health concern. Commensal Neisseria species in the oropharynx are an important reservoir of AMR genes that are transferred to N. gonorrhoeae, yet few data about AMR among commensal Neisseria in populations at risk for AMR exist. Methods: From May 2022 to December 2023, men in an HIV pre-exposure prophylaxis program in Hanoi, Vietnam, were recruited for this study. Participants self-collected oral specimens using phosphate buffer solution, for culture on LB agar media containing sucrose, vancomycin, and trimethoprim (LBVT.SNR). Oxidase-positive Gram-negative diplococci were identified using the Remel RapID NH system. Minimum inhibitory concentrations (MICs) to azithromycin, ceftriaxone, cefixime, and doxycycline were determined using Etests. Results: There were 42 male participants, the median age was 26 years and 29% (n = 12) reported using antibiotics in the past 6 months. In total, 48 Neisseria isolates were recovered; N. sicca/subflava was the most common species (50%; n = 24), followed by N. mucosa (38%; n = 18). For azithromycin, 85% (n = 41) of isolates were resistant with MICs ≥ 1 ug/mL, including 25% (n = 12) with high-level resistance (MICs ≥ 256 ug/mL of which 67% (8/12) were N. mucosa. Among non-gonococcal Neisseria isolates, the prevalence of resistance was 6% (n = 3) for ceftriaxone, 6% (n = 3) for cefixime, and 54% (n = 26) for doxycycline; the most non-susceptible isolates were N. mucosa. Conclusions: A High frequency of azithromycin resistance, moderate doxycycline resistance, and low cephalosporin resistance was found in oropharyngeal Neisseria isolates from men who have sex with men (MSM) in a pre-exposure prophylaxis (PrEP) program in Hanoi, Vietnam. N. mucosa was over-represented among resistant isolates.http://www.sciencedirect.com/science/article/pii/S2213716524004491NeisseriaCommensalsOral microbiomeAntimicrobial resistance (AMR) |
| spellingShingle | Huan Vinh Dong Paul Adamson Dieu Hoa Pham Quynh Hoa Pham Hai Ha Long Le Loc Quang Pham Hao Thi Minh Bui Giang Minh Le Jeffrey D. Klausner Antimicrobial susceptibilities of oral Neisseria from men on HIV pre-exposure prophylaxis in Hanoi, Vietnam Journal of Global Antimicrobial Resistance Neisseria Commensals Oral microbiome Antimicrobial resistance (AMR) |
| title | Antimicrobial susceptibilities of oral Neisseria from men on HIV pre-exposure prophylaxis in Hanoi, Vietnam |
| title_full | Antimicrobial susceptibilities of oral Neisseria from men on HIV pre-exposure prophylaxis in Hanoi, Vietnam |
| title_fullStr | Antimicrobial susceptibilities of oral Neisseria from men on HIV pre-exposure prophylaxis in Hanoi, Vietnam |
| title_full_unstemmed | Antimicrobial susceptibilities of oral Neisseria from men on HIV pre-exposure prophylaxis in Hanoi, Vietnam |
| title_short | Antimicrobial susceptibilities of oral Neisseria from men on HIV pre-exposure prophylaxis in Hanoi, Vietnam |
| title_sort | antimicrobial susceptibilities of oral neisseria from men on hiv pre exposure prophylaxis in hanoi vietnam |
| topic | Neisseria Commensals Oral microbiome Antimicrobial resistance (AMR) |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524004491 |
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