Phytoconstituent analysis, bioactivity, and safety evaluation of various colors of Chrysanthemum morifolium flower extracts for cosmetic application
Abstract This study evaluated the phytochemical composition, bioactivity, and safety of purple, yellow, and white Chrysanthemum × morifolium (Ramat.) Hemsl. (C. morifolium) flower extracts using a hydroglycolic solvent blend of water and propylene glycol (PG) in a 1:1 ratio as a solvent for extracti...
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Nature Portfolio
2025-02-01
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| Online Access: | https://doi.org/10.1038/s41598-025-88590-4 |
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| author | Angsana Neatpatiparn Varaporn Buraphacheep Junyaprasert Krit Thirapanmethee Veerawat Teeranachaideekul |
| author_facet | Angsana Neatpatiparn Varaporn Buraphacheep Junyaprasert Krit Thirapanmethee Veerawat Teeranachaideekul |
| author_sort | Angsana Neatpatiparn |
| collection | DOAJ |
| description | Abstract This study evaluated the phytochemical composition, bioactivity, and safety of purple, yellow, and white Chrysanthemum × morifolium (Ramat.) Hemsl. (C. morifolium) flower extracts using a hydroglycolic solvent blend of water and propylene glycol (PG) in a 1:1 ratio as a solvent for extraction. Quantitative analysis revealed that the purple and yellow flower extracts possessed significantly higher total phenolic content (TPC) and total flavonoid content (TFC) compared to the white flower extract (p < 0.05). These extracts also exhibited superior antioxidant activity, as measured by DPPH and ABTS assays. High-performance liquid chromatography (HPLC) analysis indicated that the purple extract had the highest concentration of luteolin (0.2403% w/w, p < 0.05), while the yellow extract contained the highest levels of chlorogenic acid (0.4320% w/w, p < 0.05) and caffeic acid (0.0289% w/w, p < 0.05). In vitro anti-glycation assays demonstrated that the purple extract exhibited the highest anti-glycation activity (p < 0.05). However, there was no significant difference in anti-collagenase activity between the purple and yellow extracts (p > 0.05). Safety evaluations confirmed that the extracts did not induce skin irritation or sensitization. These preliminary findings suggest the potential of C. morifolium flower extracts, particularly from yellow and purple varieties, as a promising ingredient in plant-based formulations. Initial results indicate antioxidant, anti-glycation, and anti-collagenase properties without apparent adverse skin reactions in vitro. However, further studies, including human clinical trials, are needed to confirm these biological activities and safety profiles. |
| format | Article |
| id | doaj-art-6c60df097c68426bb5f38d9f05cb468f |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-6c60df097c68426bb5f38d9f05cb468f2025-02-09T12:28:05ZengNature PortfolioScientific Reports2045-23222025-02-0115111110.1038/s41598-025-88590-4Phytoconstituent analysis, bioactivity, and safety evaluation of various colors of Chrysanthemum morifolium flower extracts for cosmetic applicationAngsana Neatpatiparn0Varaporn Buraphacheep Junyaprasert1Krit Thirapanmethee2Veerawat Teeranachaideekul3Department of Pharmacy, Faculty of Pharmacy, Mahidol UniversityDepartment of Pharmacy, Faculty of Pharmacy, Mahidol UniversityDepartment of Microbiology, Faculty of Pharmacy, Mahidol UniversityDepartment of Pharmacy, Faculty of Pharmacy, Mahidol UniversityAbstract This study evaluated the phytochemical composition, bioactivity, and safety of purple, yellow, and white Chrysanthemum × morifolium (Ramat.) Hemsl. (C. morifolium) flower extracts using a hydroglycolic solvent blend of water and propylene glycol (PG) in a 1:1 ratio as a solvent for extraction. Quantitative analysis revealed that the purple and yellow flower extracts possessed significantly higher total phenolic content (TPC) and total flavonoid content (TFC) compared to the white flower extract (p < 0.05). These extracts also exhibited superior antioxidant activity, as measured by DPPH and ABTS assays. High-performance liquid chromatography (HPLC) analysis indicated that the purple extract had the highest concentration of luteolin (0.2403% w/w, p < 0.05), while the yellow extract contained the highest levels of chlorogenic acid (0.4320% w/w, p < 0.05) and caffeic acid (0.0289% w/w, p < 0.05). In vitro anti-glycation assays demonstrated that the purple extract exhibited the highest anti-glycation activity (p < 0.05). However, there was no significant difference in anti-collagenase activity between the purple and yellow extracts (p > 0.05). Safety evaluations confirmed that the extracts did not induce skin irritation or sensitization. These preliminary findings suggest the potential of C. morifolium flower extracts, particularly from yellow and purple varieties, as a promising ingredient in plant-based formulations. Initial results indicate antioxidant, anti-glycation, and anti-collagenase properties without apparent adverse skin reactions in vitro. However, further studies, including human clinical trials, are needed to confirm these biological activities and safety profiles.https://doi.org/10.1038/s41598-025-88590-4Chrysanthemum morifolium flowersC. morifoliumAntioxidantAnti-glycationAnti-collagenaseSkin irritation |
| spellingShingle | Angsana Neatpatiparn Varaporn Buraphacheep Junyaprasert Krit Thirapanmethee Veerawat Teeranachaideekul Phytoconstituent analysis, bioactivity, and safety evaluation of various colors of Chrysanthemum morifolium flower extracts for cosmetic application Scientific Reports Chrysanthemum morifolium flowers C. morifolium Antioxidant Anti-glycation Anti-collagenase Skin irritation |
| title | Phytoconstituent analysis, bioactivity, and safety evaluation of various colors of Chrysanthemum morifolium flower extracts for cosmetic application |
| title_full | Phytoconstituent analysis, bioactivity, and safety evaluation of various colors of Chrysanthemum morifolium flower extracts for cosmetic application |
| title_fullStr | Phytoconstituent analysis, bioactivity, and safety evaluation of various colors of Chrysanthemum morifolium flower extracts for cosmetic application |
| title_full_unstemmed | Phytoconstituent analysis, bioactivity, and safety evaluation of various colors of Chrysanthemum morifolium flower extracts for cosmetic application |
| title_short | Phytoconstituent analysis, bioactivity, and safety evaluation of various colors of Chrysanthemum morifolium flower extracts for cosmetic application |
| title_sort | phytoconstituent analysis bioactivity and safety evaluation of various colors of chrysanthemum morifolium flower extracts for cosmetic application |
| topic | Chrysanthemum morifolium flowers C. morifolium Antioxidant Anti-glycation Anti-collagenase Skin irritation |
| url | https://doi.org/10.1038/s41598-025-88590-4 |
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