New nucleopolyhedrovirus isolate in the management of Spodoptera eridania
Abstract Spodoptera eridania is considered a polyphagous pest, as it attacks several crops. The aim of this study was to identify a virus isolate present in symptomatic S. eridania caterpillars. The virus sample was extracted and OBs purification performed. Viral DNA was extracted using the PureLink...
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Academia Brasileira de Ciências
2025-02-01
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author | ALIXELHE P. DAMASCENA FERNANDO Z. MADALON LUIS M. DE ARAUJO JUNIOR DIRCEU PRATISSOLI CARLOS EDUARDO C. PAIVA LORENA C. MACHADO HUGO GONÇALVES JUNIOR VINÍCIUS H. BELLO |
author_facet | ALIXELHE P. DAMASCENA FERNANDO Z. MADALON LUIS M. DE ARAUJO JUNIOR DIRCEU PRATISSOLI CARLOS EDUARDO C. PAIVA LORENA C. MACHADO HUGO GONÇALVES JUNIOR VINÍCIUS H. BELLO |
author_sort | ALIXELHE P. DAMASCENA |
collection | DOAJ |
description | Abstract Spodoptera eridania is considered a polyphagous pest, as it attacks several crops. The aim of this study was to identify a virus isolate present in symptomatic S. eridania caterpillars. The virus sample was extracted and OBs purification performed. Viral DNA was extracted using the PureLink Viral RNA/DNA Mini Kit (ThermoFisher) and primers prl8-1 and prl8-1B were used for amplification of the gene fragment. The isolate was identified showing high genetic similarity with the isolate “Spodoptera eridania nucleopolyhedrovirus isolate CNPSo-165”, characterizing itself as a novelty in the scenario and as promising, adding the tactics available for the integrated management of S. eridania. |
format | Article |
id | doaj-art-6c75a14a285644679e6fb7e637e9353c |
institution | Kabale University |
issn | 1678-2690 |
language | English |
publishDate | 2025-02-01 |
publisher | Academia Brasileira de Ciências |
record_format | Article |
series | Anais da Academia Brasileira de Ciências |
spelling | doaj-art-6c75a14a285644679e6fb7e637e9353c2025-02-11T07:42:08ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902025-02-0197110.1590/0001-3765202520230077New nucleopolyhedrovirus isolate in the management of Spodoptera eridaniaALIXELHE P. DAMASCENAhttps://orcid.org/0000-0003-1374-5119FERNANDO Z. MADALONhttps://orcid.org/0000-0001-7140-7615LUIS M. DE ARAUJO JUNIORhttps://orcid.org/0000-0003-3354-9465DIRCEU PRATISSOLIhttps://orcid.org/0000-0003-4485-1491CARLOS EDUARDO C. PAIVAhttps://orcid.org/0000-0002-6086-9600LORENA C. MACHADOhttps://orcid.org/0000-0002-8126-167XHUGO GONÇALVES JUNIORhttps://orcid.org/0000-0002-6780-6610VINÍCIUS H. BELLOhttps://orcid.org/0000-0002-2869-8119Abstract Spodoptera eridania is considered a polyphagous pest, as it attacks several crops. The aim of this study was to identify a virus isolate present in symptomatic S. eridania caterpillars. The virus sample was extracted and OBs purification performed. Viral DNA was extracted using the PureLink Viral RNA/DNA Mini Kit (ThermoFisher) and primers prl8-1 and prl8-1B were used for amplification of the gene fragment. The isolate was identified showing high genetic similarity with the isolate “Spodoptera eridania nucleopolyhedrovirus isolate CNPSo-165”, characterizing itself as a novelty in the scenario and as promising, adding the tactics available for the integrated management of S. eridania.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652025000100901&lng=en&tlng=enBaculovirusIntegrated pest managementVirus identificationPathogenicity |
spellingShingle | ALIXELHE P. DAMASCENA FERNANDO Z. MADALON LUIS M. DE ARAUJO JUNIOR DIRCEU PRATISSOLI CARLOS EDUARDO C. PAIVA LORENA C. MACHADO HUGO GONÇALVES JUNIOR VINÍCIUS H. BELLO New nucleopolyhedrovirus isolate in the management of Spodoptera eridania Anais da Academia Brasileira de Ciências Baculovirus Integrated pest management Virus identification Pathogenicity |
title | New nucleopolyhedrovirus isolate in the management of Spodoptera eridania |
title_full | New nucleopolyhedrovirus isolate in the management of Spodoptera eridania |
title_fullStr | New nucleopolyhedrovirus isolate in the management of Spodoptera eridania |
title_full_unstemmed | New nucleopolyhedrovirus isolate in the management of Spodoptera eridania |
title_short | New nucleopolyhedrovirus isolate in the management of Spodoptera eridania |
title_sort | new nucleopolyhedrovirus isolate in the management of spodoptera eridania |
topic | Baculovirus Integrated pest management Virus identification Pathogenicity |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652025000100901&lng=en&tlng=en |
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