New nucleopolyhedrovirus isolate in the management of Spodoptera eridania

Abstract Spodoptera eridania is considered a polyphagous pest, as it attacks several crops. The aim of this study was to identify a virus isolate present in symptomatic S. eridania caterpillars. The virus sample was extracted and OBs purification performed. Viral DNA was extracted using the PureLink...

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Main Authors: ALIXELHE P. DAMASCENA, FERNANDO Z. MADALON, LUIS M. DE ARAUJO JUNIOR, DIRCEU PRATISSOLI, CARLOS EDUARDO C. PAIVA, LORENA C. MACHADO, HUGO GONÇALVES JUNIOR, VINÍCIUS H. BELLO
Format: Article
Language:English
Published: Academia Brasileira de Ciências 2025-02-01
Series:Anais da Academia Brasileira de Ciências
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Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652025000100901&lng=en&tlng=en
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author ALIXELHE P. DAMASCENA
FERNANDO Z. MADALON
LUIS M. DE ARAUJO JUNIOR
DIRCEU PRATISSOLI
CARLOS EDUARDO C. PAIVA
LORENA C. MACHADO
HUGO GONÇALVES JUNIOR
VINÍCIUS H. BELLO
author_facet ALIXELHE P. DAMASCENA
FERNANDO Z. MADALON
LUIS M. DE ARAUJO JUNIOR
DIRCEU PRATISSOLI
CARLOS EDUARDO C. PAIVA
LORENA C. MACHADO
HUGO GONÇALVES JUNIOR
VINÍCIUS H. BELLO
author_sort ALIXELHE P. DAMASCENA
collection DOAJ
description Abstract Spodoptera eridania is considered a polyphagous pest, as it attacks several crops. The aim of this study was to identify a virus isolate present in symptomatic S. eridania caterpillars. The virus sample was extracted and OBs purification performed. Viral DNA was extracted using the PureLink Viral RNA/DNA Mini Kit (ThermoFisher) and primers prl8-1 and prl8-1B were used for amplification of the gene fragment. The isolate was identified showing high genetic similarity with the isolate “Spodoptera eridania nucleopolyhedrovirus isolate CNPSo-165”, characterizing itself as a novelty in the scenario and as promising, adding the tactics available for the integrated management of S. eridania.
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spelling doaj-art-6c75a14a285644679e6fb7e637e9353c2025-02-11T07:42:08ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902025-02-0197110.1590/0001-3765202520230077New nucleopolyhedrovirus isolate in the management of Spodoptera eridaniaALIXELHE P. DAMASCENAhttps://orcid.org/0000-0003-1374-5119FERNANDO Z. MADALONhttps://orcid.org/0000-0001-7140-7615LUIS M. DE ARAUJO JUNIORhttps://orcid.org/0000-0003-3354-9465DIRCEU PRATISSOLIhttps://orcid.org/0000-0003-4485-1491CARLOS EDUARDO C. PAIVAhttps://orcid.org/0000-0002-6086-9600LORENA C. MACHADOhttps://orcid.org/0000-0002-8126-167XHUGO GONÇALVES JUNIORhttps://orcid.org/0000-0002-6780-6610VINÍCIUS H. BELLOhttps://orcid.org/0000-0002-2869-8119Abstract Spodoptera eridania is considered a polyphagous pest, as it attacks several crops. The aim of this study was to identify a virus isolate present in symptomatic S. eridania caterpillars. The virus sample was extracted and OBs purification performed. Viral DNA was extracted using the PureLink Viral RNA/DNA Mini Kit (ThermoFisher) and primers prl8-1 and prl8-1B were used for amplification of the gene fragment. The isolate was identified showing high genetic similarity with the isolate “Spodoptera eridania nucleopolyhedrovirus isolate CNPSo-165”, characterizing itself as a novelty in the scenario and as promising, adding the tactics available for the integrated management of S. eridania.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652025000100901&lng=en&tlng=enBaculovirusIntegrated pest managementVirus identificationPathogenicity
spellingShingle ALIXELHE P. DAMASCENA
FERNANDO Z. MADALON
LUIS M. DE ARAUJO JUNIOR
DIRCEU PRATISSOLI
CARLOS EDUARDO C. PAIVA
LORENA C. MACHADO
HUGO GONÇALVES JUNIOR
VINÍCIUS H. BELLO
New nucleopolyhedrovirus isolate in the management of Spodoptera eridania
Anais da Academia Brasileira de Ciências
Baculovirus
Integrated pest management
Virus identification
Pathogenicity
title New nucleopolyhedrovirus isolate in the management of Spodoptera eridania
title_full New nucleopolyhedrovirus isolate in the management of Spodoptera eridania
title_fullStr New nucleopolyhedrovirus isolate in the management of Spodoptera eridania
title_full_unstemmed New nucleopolyhedrovirus isolate in the management of Spodoptera eridania
title_short New nucleopolyhedrovirus isolate in the management of Spodoptera eridania
title_sort new nucleopolyhedrovirus isolate in the management of spodoptera eridania
topic Baculovirus
Integrated pest management
Virus identification
Pathogenicity
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652025000100901&lng=en&tlng=en
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