Role of gut microbiota and fecal metabolites in the protective effect of soybean pulp-rich diet against estrogen-induced cholestasis in rats
This study was designed to explore the role of gut microbiota and its metabolites in the treatment of estrogen-induced cholestasis (EIC) in rats with a soybean pulp-rich diet and to clarify the effects of daidzein (DAI), a principal active ingredient of soybean pulp. The findings demonstrated that t...
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Elsevier
2025-01-01
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| Series: | Current Research in Food Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927125000218 |
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| author | Jiawei Wang Qichao Hu Jianying Wang Liwei Lang Shizhang Wei Haotian Li Manyi Jing Xiao Ma Yanling Zhao Xuelin Zhou |
| author_facet | Jiawei Wang Qichao Hu Jianying Wang Liwei Lang Shizhang Wei Haotian Li Manyi Jing Xiao Ma Yanling Zhao Xuelin Zhou |
| author_sort | Jiawei Wang |
| collection | DOAJ |
| description | This study was designed to explore the role of gut microbiota and its metabolites in the treatment of estrogen-induced cholestasis (EIC) in rats with a soybean pulp-rich diet and to clarify the effects of daidzein (DAI), a principal active ingredient of soybean pulp. The findings demonstrated that the soybean pulp-rich diet could relieve cholestasis by decreasing the levels of total bile acids (TBA) and alkaline phosphatase and enhancing the bile flow rate. Through gut microbiota and metabolomics analyses, it was revealed that this diet might alter the abundances of certain bacterial taxa including Akkermansia, Bacteroides, and Turicibacter, thus influencing lipid metabolism, tryptophan metabolism, and steroid metabolism, which led to disparities between the groups fed with and without the soybean pulp-rich diet. Moreover, the soybean pulp-rich diet could modulate the abundances of Prevotella spp. and Tyzzerella, reducing EIC by regulating lipid metabolism and short-chain fatty acids synthesis. Notably, DAI treatment significantly alleviated the abnormalities in serum TBA, alanine aminotransferase, and aspartate aminotransferase levels and mitigated the liver tissue damage in the EIC model. In summary, during cholestasis, variations in gut microbiota and metabolite profiles occurred. The intervention of soybean pulp affected the abundances of bacteria (such as Prevotella spp. and Tyzzerella) and regulated lipid metabolism-related pathways. Importantly, DAI was identified as a crucial component for the protective effects associated with the soybean pulp diet. |
| format | Article |
| id | doaj-art-6d3d2d0402684d32b5d70a8e9baea4e6 |
| institution | Kabale University |
| issn | 2665-9271 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj-art-6d3d2d0402684d32b5d70a8e9baea4e62025-02-10T04:34:55ZengElsevierCurrent Research in Food Science2665-92712025-01-0110100990Role of gut microbiota and fecal metabolites in the protective effect of soybean pulp-rich diet against estrogen-induced cholestasis in ratsJiawei Wang0Qichao Hu1Jianying Wang2Liwei Lang3Shizhang Wei4Haotian Li5Manyi Jing6Xiao Ma7Yanling Zhao8Xuelin Zhou9State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China; Department of Pharmacy, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Information, Medical Supplies Center of PLA General Hospital, Beijing, ChinaDepartment of Pharmacy, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaDepartment of Pharmacy, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaDepartment of Pharmacy, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaDepartment of Pharmacy, The Fifth Medical Center of PLA General Hospital, Beijing, ChinaState Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China; Corresponding author.Department of Pharmacy, The Fifth Medical Center of PLA General Hospital, Beijing, China; Corresponding author.State Key Laboratory of Druggability Evaluation and Systematic Translational Medicine, Tianjin Institute of Pharmaceutical Research, Tianjin, China; Department of Pharmacology, School of Basic Medical Sciences, Capital Medical University, Beijing, China; Corresponding author. State Key Laboratory of Druggability Evaluation and Systematic Translational Medicine, Tianjin Institute of Pharmaceutical Research & Department of Pharmacology, School of Basic Medical Sciences, Capital Medical University, China.This study was designed to explore the role of gut microbiota and its metabolites in the treatment of estrogen-induced cholestasis (EIC) in rats with a soybean pulp-rich diet and to clarify the effects of daidzein (DAI), a principal active ingredient of soybean pulp. The findings demonstrated that the soybean pulp-rich diet could relieve cholestasis by decreasing the levels of total bile acids (TBA) and alkaline phosphatase and enhancing the bile flow rate. Through gut microbiota and metabolomics analyses, it was revealed that this diet might alter the abundances of certain bacterial taxa including Akkermansia, Bacteroides, and Turicibacter, thus influencing lipid metabolism, tryptophan metabolism, and steroid metabolism, which led to disparities between the groups fed with and without the soybean pulp-rich diet. Moreover, the soybean pulp-rich diet could modulate the abundances of Prevotella spp. and Tyzzerella, reducing EIC by regulating lipid metabolism and short-chain fatty acids synthesis. Notably, DAI treatment significantly alleviated the abnormalities in serum TBA, alanine aminotransferase, and aspartate aminotransferase levels and mitigated the liver tissue damage in the EIC model. In summary, during cholestasis, variations in gut microbiota and metabolite profiles occurred. The intervention of soybean pulp affected the abundances of bacteria (such as Prevotella spp. and Tyzzerella) and regulated lipid metabolism-related pathways. Importantly, DAI was identified as a crucial component for the protective effects associated with the soybean pulp diet.http://www.sciencedirect.com/science/article/pii/S2665927125000218Soybean pulpDaidzeinCholestasisEstrogenIntestinal floraMetabolomics |
| spellingShingle | Jiawei Wang Qichao Hu Jianying Wang Liwei Lang Shizhang Wei Haotian Li Manyi Jing Xiao Ma Yanling Zhao Xuelin Zhou Role of gut microbiota and fecal metabolites in the protective effect of soybean pulp-rich diet against estrogen-induced cholestasis in rats Current Research in Food Science Soybean pulp Daidzein Cholestasis Estrogen Intestinal flora Metabolomics |
| title | Role of gut microbiota and fecal metabolites in the protective effect of soybean pulp-rich diet against estrogen-induced cholestasis in rats |
| title_full | Role of gut microbiota and fecal metabolites in the protective effect of soybean pulp-rich diet against estrogen-induced cholestasis in rats |
| title_fullStr | Role of gut microbiota and fecal metabolites in the protective effect of soybean pulp-rich diet against estrogen-induced cholestasis in rats |
| title_full_unstemmed | Role of gut microbiota and fecal metabolites in the protective effect of soybean pulp-rich diet against estrogen-induced cholestasis in rats |
| title_short | Role of gut microbiota and fecal metabolites in the protective effect of soybean pulp-rich diet against estrogen-induced cholestasis in rats |
| title_sort | role of gut microbiota and fecal metabolites in the protective effect of soybean pulp rich diet against estrogen induced cholestasis in rats |
| topic | Soybean pulp Daidzein Cholestasis Estrogen Intestinal flora Metabolomics |
| url | http://www.sciencedirect.com/science/article/pii/S2665927125000218 |
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