Assessment of Serum Dynamic Thiol/Disulfide Homeostasis and Oxidative/Nitrosative Stress in Patients with Crohn’s Disease
Background/Aims: Crohn’s disease (CD) is a major subtype of chronic relapsing inflammatory gastrointestinal disorders. In this study, we assessed the possible contributions of serum oxidative/nitrosative stress and dynamic thiol/disulfide homeostasis to CD pathogenesis. Materials and Methods: Pat...
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| Format: | Article |
| Language: | English |
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AVES
2025-02-01
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| Series: | The Turkish Journal of Gastroenterology |
| Online Access: | https://www.turkjgastroenterol.org/en/assessment-of-serum-dynamic-thiol-disulfide-homeostasis-and-oxidative-nitrosative-stress-in-patients-with-crohn-s-disease-137297 |
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| author | Ancel Aysun Bağdaş Sezgin Barutçu Ahmet Saracaloğlu Abdullah Tuncay Demiryürek |
| author_facet | Ancel Aysun Bağdaş Sezgin Barutçu Ahmet Saracaloğlu Abdullah Tuncay Demiryürek |
| author_sort | Ancel Aysun Bağdaş |
| collection | DOAJ |
| description | Background/Aims: Crohn’s disease (CD) is a major subtype of chronic relapsing inflammatory gastrointestinal disorders. In this study, we assessed the possible contributions of serum oxidative/nitrosative stress and dynamic thiol/disulfide homeostasis to CD pathogenesis.
Materials and Methods: Patients with active CD (A-CD) at onset (n = 38), CD patients in the remission (R-CD) (n = 38), and healthy controls (n = 38) were prospectively included in this study. Serum oxidative/nitrosative parameters as well as total thiol and native thiol levels were analyzed.
Results: We observed significant augmentation in nitric oxide (NO) levels in both A-CD and R-CD patients compared to healthy controls. We detected marked reductions in the 3-nitrotyrosine levels in the patient groups. Glutathione, glutathione peroxidase, and myeloperoxidase levels were observed to be significantly lower in both the active and remission groups (P < .001). In the A-CD group, native thiol (P < .001) and total thiol (P < .01) levels were lower, and disulfide levels were higher than those of the control group (P < .01), while the native thiol/total thiol ratio was reduced and disulfide/total thiol (P < .001) and disulfide/native thiol (P < .001) ratios were elevated. Remarkably, no change in dynamic thiol/disulfide homeostasis was found in the R-CD group.
Conclusion: Our results showed increased serum NO levels and decreased antioxidant enzymes, particularly during the active phase of CD. Determination of thiol/disulfide homeostasis could help differentiate between the active and remission phases of the disease. Thiol/ disulfide parameters can be used as biomarkers for A-CD. |
| format | Article |
| id | doaj-art-6db94b39116946638709271062789f99 |
| institution | Kabale University |
| issn | 2148-5607 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | AVES |
| record_format | Article |
| series | The Turkish Journal of Gastroenterology |
| spelling | doaj-art-6db94b39116946638709271062789f992025-02-11T13:59:16ZengAVESThe Turkish Journal of Gastroenterology2148-56072025-02-0136211412310.5152/tjg.2024.23519Assessment of Serum Dynamic Thiol/Disulfide Homeostasis and Oxidative/Nitrosative Stress in Patients with Crohn’s DiseaseAncel Aysun Bağdaş0https://orcid.org/0000-0003-1618-4644Sezgin Barutçu1https://orcid.org/0000-0003-0823-3215Ahmet Saracaloğlu2https://orcid.org/0000-0003-2534-6051Abdullah Tuncay Demiryürek3https://orcid.org/0000-0002-9994-8541Department of Gastroenterology,Gaziantep University Faculty of Medicine, Gaziantep, TürkiyeDepartment of Gastroenterology,Gaziantep University Faculty of Medicine, Gaziantep, TürkiyeDepartment of Medical Pharmacology, Gaziantep University Faculty of Medicine, Gaziantep, TürkiyeDepartment of Medical Pharmacology, Gaziantep University Faculty of Medicine, Gaziantep, TürkiyeBackground/Aims: Crohn’s disease (CD) is a major subtype of chronic relapsing inflammatory gastrointestinal disorders. In this study, we assessed the possible contributions of serum oxidative/nitrosative stress and dynamic thiol/disulfide homeostasis to CD pathogenesis. Materials and Methods: Patients with active CD (A-CD) at onset (n = 38), CD patients in the remission (R-CD) (n = 38), and healthy controls (n = 38) were prospectively included in this study. Serum oxidative/nitrosative parameters as well as total thiol and native thiol levels were analyzed. Results: We observed significant augmentation in nitric oxide (NO) levels in both A-CD and R-CD patients compared to healthy controls. We detected marked reductions in the 3-nitrotyrosine levels in the patient groups. Glutathione, glutathione peroxidase, and myeloperoxidase levels were observed to be significantly lower in both the active and remission groups (P < .001). In the A-CD group, native thiol (P < .001) and total thiol (P < .01) levels were lower, and disulfide levels were higher than those of the control group (P < .01), while the native thiol/total thiol ratio was reduced and disulfide/total thiol (P < .001) and disulfide/native thiol (P < .001) ratios were elevated. Remarkably, no change in dynamic thiol/disulfide homeostasis was found in the R-CD group. Conclusion: Our results showed increased serum NO levels and decreased antioxidant enzymes, particularly during the active phase of CD. Determination of thiol/disulfide homeostasis could help differentiate between the active and remission phases of the disease. Thiol/ disulfide parameters can be used as biomarkers for A-CD.https://www.turkjgastroenterol.org/en/assessment-of-serum-dynamic-thiol-disulfide-homeostasis-and-oxidative-nitrosative-stress-in-patients-with-crohn-s-disease-137297 |
| spellingShingle | Ancel Aysun Bağdaş Sezgin Barutçu Ahmet Saracaloğlu Abdullah Tuncay Demiryürek Assessment of Serum Dynamic Thiol/Disulfide Homeostasis and Oxidative/Nitrosative Stress in Patients with Crohn’s Disease The Turkish Journal of Gastroenterology |
| title | Assessment of Serum Dynamic Thiol/Disulfide Homeostasis and Oxidative/Nitrosative Stress in Patients with Crohn’s Disease |
| title_full | Assessment of Serum Dynamic Thiol/Disulfide Homeostasis and Oxidative/Nitrosative Stress in Patients with Crohn’s Disease |
| title_fullStr | Assessment of Serum Dynamic Thiol/Disulfide Homeostasis and Oxidative/Nitrosative Stress in Patients with Crohn’s Disease |
| title_full_unstemmed | Assessment of Serum Dynamic Thiol/Disulfide Homeostasis and Oxidative/Nitrosative Stress in Patients with Crohn’s Disease |
| title_short | Assessment of Serum Dynamic Thiol/Disulfide Homeostasis and Oxidative/Nitrosative Stress in Patients with Crohn’s Disease |
| title_sort | assessment of serum dynamic thiol disulfide homeostasis and oxidative nitrosative stress in patients with crohn s disease |
| url | https://www.turkjgastroenterol.org/en/assessment-of-serum-dynamic-thiol-disulfide-homeostasis-and-oxidative-nitrosative-stress-in-patients-with-crohn-s-disease-137297 |
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