Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure
Abstract Constructing a dense vascular endothelial network within engineered tissue is crucial for successful engraftment. The present study investigated the effects of air-compressing intermittent positive pressure (IPP) on co-cultured mesenchymal stem cells and vascular endothelial cells and evalu...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07627-6 |
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| _version_ | 1823861726621204480 |
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| author | Misako Katsuura Jun Homma Yuhei Higashi Hidekazu Sekine Tatsuya Shimizu |
| author_facet | Misako Katsuura Jun Homma Yuhei Higashi Hidekazu Sekine Tatsuya Shimizu |
| author_sort | Misako Katsuura |
| collection | DOAJ |
| description | Abstract Constructing a dense vascular endothelial network within engineered tissue is crucial for successful engraftment. The present study investigated the effects of air-compressing intermittent positive pressure (IPP) on co-cultured mesenchymal stem cells and vascular endothelial cells and evaluated the potential of IPP-cultured cell sheets for transplantation therapy. The results demonstrated that the IPP (+) group exhibited a denser vascular endothelial network and significantly increased cell sheet thickness compared to the IPP (-) group. Furthermore, in vivo experiments showed that IPP-cultured cell sheets enhanced the secretion of Gaussian luciferase by genetically modified mesenchymal stem cells. These findings highlight the IPP method as a technique that simultaneously enables the thickening of planar tissues and the construction of vascular networks. This approach demonstrates promise for fabricating functional, transplantable, and thick tissues with dense vascularization and a high capacity for protein secretion, paving the way for novel applications in regenerative medicine. |
| format | Article |
| id | doaj-art-6dcca04b755d4f148b92cbbf7a0ab094 |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-6dcca04b755d4f148b92cbbf7a0ab0942025-02-09T12:50:36ZengNature PortfolioCommunications Biology2399-36422025-02-018111710.1038/s42003-025-07627-6Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressureMisako Katsuura0Jun Homma1Yuhei Higashi2Hidekazu Sekine3Tatsuya Shimizu4Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women’s Medical UniversityInstitute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women’s Medical UniversityTokaihit Co. LtdInstitute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women’s Medical UniversityInstitute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women’s Medical UniversityAbstract Constructing a dense vascular endothelial network within engineered tissue is crucial for successful engraftment. The present study investigated the effects of air-compressing intermittent positive pressure (IPP) on co-cultured mesenchymal stem cells and vascular endothelial cells and evaluated the potential of IPP-cultured cell sheets for transplantation therapy. The results demonstrated that the IPP (+) group exhibited a denser vascular endothelial network and significantly increased cell sheet thickness compared to the IPP (-) group. Furthermore, in vivo experiments showed that IPP-cultured cell sheets enhanced the secretion of Gaussian luciferase by genetically modified mesenchymal stem cells. These findings highlight the IPP method as a technique that simultaneously enables the thickening of planar tissues and the construction of vascular networks. This approach demonstrates promise for fabricating functional, transplantable, and thick tissues with dense vascularization and a high capacity for protein secretion, paving the way for novel applications in regenerative medicine.https://doi.org/10.1038/s42003-025-07627-6 |
| spellingShingle | Misako Katsuura Jun Homma Yuhei Higashi Hidekazu Sekine Tatsuya Shimizu Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure Communications Biology |
| title | Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure |
| title_full | Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure |
| title_fullStr | Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure |
| title_full_unstemmed | Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure |
| title_short | Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure |
| title_sort | densely vascularized thick 3d tissue shows enhanced protein secretion constructed with intermittent positive pressure |
| url | https://doi.org/10.1038/s42003-025-07627-6 |
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