Dose-dependent effects of hydroquinone on liver injury and lipid dysregulation based on SCD1/AMPK signaling pathway in C57BL/6 mice
Hydroquinone (HQ) is extensively utilized in various industrial applications and as a dermatological agent; however, its metabolic processes and toxicological effects, particularly in the liver, remain insufficiently understood. This study aimed to investigate the impact of different doses of HQ on...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325000600 |
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| author | Lijun Zou Fen Lin Jinying Wen Jinyu Huang Yue Tan Hui Huang Gonghua Hu |
| author_facet | Lijun Zou Fen Lin Jinying Wen Jinyu Huang Yue Tan Hui Huang Gonghua Hu |
| author_sort | Lijun Zou |
| collection | DOAJ |
| description | Hydroquinone (HQ) is extensively utilized in various industrial applications and as a dermatological agent; however, its metabolic processes and toxicological effects, particularly in the liver, remain insufficiently understood. This study aimed to investigate the impact of different doses of HQ on liver injury and lipid metabolism in C57BL/6 mice, with a specific focus on the SCD1/AMPK signaling pathway. We administered HQ at doses of 0, 12.5, 25.0, and 50.0 mg/kg over a period of 13 weeks, followed by biochemical analyses, RNA sequencing, and Western blotting to elucidate the underlying mechanisms. Our findings indicated that lower doses of HQ (12.5 and 25.0 mg/kg) led to lipid accumulation in the liver, accompanied by increased liver TG and serum TC. In contrast, the highest dose (50.0 mg/kg) resulted in elevated liver enzyme levels, indicative of liver damage, while lipid levels decreased. Notably, the mRNA or protein levels of SCD1 were upregulated in response to the lower doses of HQ (12.5 and 25.0 mg/kg), whereas AMPK activation and enhanced autophagy were observed in the 50.0 mg/kg HQ-treated group, reflecting an energy stress response. These findings suggest that lipid dysregulation may serve as an early indicator of HQ-induced liver injury, and that the SCD1/AMPK pathway may play a protective role against chemically induced hepatotoxicity. This study offers new insights into the toxicological effects of HQ on the liver and underscores the necessity for further exploration of the protective mechanisms of SCD1 and AMPK in mitigating HQ-induced hepatotoxicity. |
| format | Article |
| id | doaj-art-6e32de03026f4297bb1239cb01ee8677 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-6e32de03026f4297bb1239cb01ee86772025-02-12T05:30:05ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117724Dose-dependent effects of hydroquinone on liver injury and lipid dysregulation based on SCD1/AMPK signaling pathway in C57BL/6 miceLijun Zou0Fen Lin1Jinying Wen2Jinyu Huang3Yue Tan4Hui Huang5Gonghua Hu6School of Public Health and Health Management, Gannan Medical University, Ganzhou, Jiangxi 341000, PR ChinaSchool of Public Health and Health Management, Gannan Medical University, Ganzhou, Jiangxi 341000, PR ChinaSchool of Public Health and Health Management, Gannan Medical University, Ganzhou, Jiangxi 341000, PR China; Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases of Ministry of Education, Gannan Medical University, Ganzhou, Jiangxi 341000, PR ChinaKey Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases of Ministry of Education, Gannan Medical University, Ganzhou, Jiangxi 341000, PR ChinaSchool of Public Health and Health Management, Gannan Medical University, Ganzhou, Jiangxi 341000, PR ChinaSchool of Public Health and Health Management, Gannan Medical University, Ganzhou, Jiangxi 341000, PR ChinaSchool of Public Health and Health Management, Gannan Medical University, Ganzhou, Jiangxi 341000, PR China; Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases of Ministry of Education, Gannan Medical University, Ganzhou, Jiangxi 341000, PR China; Corresponding author at: School of Public Health and Health Management, Gannan Medical University, Ganzhou, Jiangxi 341000, PR China.Hydroquinone (HQ) is extensively utilized in various industrial applications and as a dermatological agent; however, its metabolic processes and toxicological effects, particularly in the liver, remain insufficiently understood. This study aimed to investigate the impact of different doses of HQ on liver injury and lipid metabolism in C57BL/6 mice, with a specific focus on the SCD1/AMPK signaling pathway. We administered HQ at doses of 0, 12.5, 25.0, and 50.0 mg/kg over a period of 13 weeks, followed by biochemical analyses, RNA sequencing, and Western blotting to elucidate the underlying mechanisms. Our findings indicated that lower doses of HQ (12.5 and 25.0 mg/kg) led to lipid accumulation in the liver, accompanied by increased liver TG and serum TC. In contrast, the highest dose (50.0 mg/kg) resulted in elevated liver enzyme levels, indicative of liver damage, while lipid levels decreased. Notably, the mRNA or protein levels of SCD1 were upregulated in response to the lower doses of HQ (12.5 and 25.0 mg/kg), whereas AMPK activation and enhanced autophagy were observed in the 50.0 mg/kg HQ-treated group, reflecting an energy stress response. These findings suggest that lipid dysregulation may serve as an early indicator of HQ-induced liver injury, and that the SCD1/AMPK pathway may play a protective role against chemically induced hepatotoxicity. This study offers new insights into the toxicological effects of HQ on the liver and underscores the necessity for further exploration of the protective mechanisms of SCD1 and AMPK in mitigating HQ-induced hepatotoxicity.http://www.sciencedirect.com/science/article/pii/S0147651325000600HydroquinoneLiver injuryLipid disregulationSCD1AMPKDose-dependent |
| spellingShingle | Lijun Zou Fen Lin Jinying Wen Jinyu Huang Yue Tan Hui Huang Gonghua Hu Dose-dependent effects of hydroquinone on liver injury and lipid dysregulation based on SCD1/AMPK signaling pathway in C57BL/6 mice Ecotoxicology and Environmental Safety Hydroquinone Liver injury Lipid disregulation SCD1 AMPK Dose-dependent |
| title | Dose-dependent effects of hydroquinone on liver injury and lipid dysregulation based on SCD1/AMPK signaling pathway in C57BL/6 mice |
| title_full | Dose-dependent effects of hydroquinone on liver injury and lipid dysregulation based on SCD1/AMPK signaling pathway in C57BL/6 mice |
| title_fullStr | Dose-dependent effects of hydroquinone on liver injury and lipid dysregulation based on SCD1/AMPK signaling pathway in C57BL/6 mice |
| title_full_unstemmed | Dose-dependent effects of hydroquinone on liver injury and lipid dysregulation based on SCD1/AMPK signaling pathway in C57BL/6 mice |
| title_short | Dose-dependent effects of hydroquinone on liver injury and lipid dysregulation based on SCD1/AMPK signaling pathway in C57BL/6 mice |
| title_sort | dose dependent effects of hydroquinone on liver injury and lipid dysregulation based on scd1 ampk signaling pathway in c57bl 6 mice |
| topic | Hydroquinone Liver injury Lipid disregulation SCD1 AMPK Dose-dependent |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325000600 |
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