Interventional real-time molecular MRI for targeting early myocardial injury in a pig model
Abstract Myocardial ischemia induces tissue injury with subsequent inflammation and recruitment of immune cells. Besides myocardial tissue characterization, magnetic resonance imaging (MRI) allows for functional assessment using molecular imaging contrast agents. Here, we assessed ischemic cardiac l...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | npj Imaging |
| Online Access: | https://doi.org/10.1038/s44303-025-00069-z |
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| author | Timo Heidt Simon Reiss Julien Thielmann Christian Weber Alexander Maier Thomas Lottner Heidi R. Cristina-Schmitz Timon Bühler Diana Chiang Claus Jülicher Carolin Wadle Ingo Hilgendorf Dennis Wolf Gavin Tumlinson Luis Hortells Dirk Westermann Michael Bock Constantin von zur Mühlen |
| author_facet | Timo Heidt Simon Reiss Julien Thielmann Christian Weber Alexander Maier Thomas Lottner Heidi R. Cristina-Schmitz Timon Bühler Diana Chiang Claus Jülicher Carolin Wadle Ingo Hilgendorf Dennis Wolf Gavin Tumlinson Luis Hortells Dirk Westermann Michael Bock Constantin von zur Mühlen |
| author_sort | Timo Heidt |
| collection | DOAJ |
| description | Abstract Myocardial ischemia induces tissue injury with subsequent inflammation and recruitment of immune cells. Besides myocardial tissue characterization, magnetic resonance imaging (MRI) allows for functional assessment using molecular imaging contrast agents. Here, we assessed ischemic cardiac lesions non-invasively directly after ischemia/reperfusion (I/R) in a porcine model by advanced MRI techniques and molecular imaging, targeting the cell adhesion molecule P-selectin functionalized with microparticles of iron oxide (MPIO). We used a closed-chest model of I/R by temporary coronary balloon-occlusion, real time 3T MRI-guided coronary injection of MPIO-based contrast agents, as well as injury, edema and iron-sensitive MRI. Within the first hours after I/R, we found T1 mapping to be most sensitive for tissue injury, with no changes in edema-sensitive MRI. Intriguingly, P-selectin MPIO contrast agent selectively enhanced the ischemic area in iron-sensitive MRI. In conclusion, this approach allows for sensitive detection of early myocardial inflammation beyond traditional edema-sensitive imaging. |
| format | Article |
| id | doaj-art-6f19c58ecc994fd98136b8f8419ab32d |
| institution | Kabale University |
| issn | 2948-197X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Imaging |
| spelling | doaj-art-6f19c58ecc994fd98136b8f8419ab32d2025-02-09T12:50:13ZengNature Portfolionpj Imaging2948-197X2025-02-013111010.1038/s44303-025-00069-zInterventional real-time molecular MRI for targeting early myocardial injury in a pig modelTimo Heidt0Simon Reiss1Julien Thielmann2Christian Weber3Alexander Maier4Thomas Lottner5Heidi R. Cristina-Schmitz6Timon Bühler7Diana Chiang8Claus Jülicher9Carolin Wadle10Ingo Hilgendorf11Dennis Wolf12Gavin Tumlinson13Luis Hortells14Dirk Westermann15Michael Bock16Constantin von zur Mühlen17Department of Cardiology, Medical Center University of FreiburgFaculty of Medicine, University of FreiburgDepartment of Cardiology, Medical Center University of FreiburgDepartment of Cardiology, Medical Center University of FreiburgDepartment of Cardiology, Medical Center University of FreiburgFaculty of Medicine, University of FreiburgFaculty of Medicine, University of FreiburgDepartment of Cardiology, Medical Center University of FreiburgDepartment of Cardiology, Medical Center University of FreiburgDepartment of Cardiology, Medical Center University of FreiburgDepartment of Cardiology, Medical Center University of FreiburgDepartment of Cardiology, Medical Center University of FreiburgDepartment of Cardiology, Medical Center University of FreiburgFaculty of Medicine, University of FreiburgFaculty of Medicine, University of FreiburgDepartment of Cardiology, Medical Center University of FreiburgFaculty of Medicine, University of FreiburgDepartment of Cardiology, Medical Center University of FreiburgAbstract Myocardial ischemia induces tissue injury with subsequent inflammation and recruitment of immune cells. Besides myocardial tissue characterization, magnetic resonance imaging (MRI) allows for functional assessment using molecular imaging contrast agents. Here, we assessed ischemic cardiac lesions non-invasively directly after ischemia/reperfusion (I/R) in a porcine model by advanced MRI techniques and molecular imaging, targeting the cell adhesion molecule P-selectin functionalized with microparticles of iron oxide (MPIO). We used a closed-chest model of I/R by temporary coronary balloon-occlusion, real time 3T MRI-guided coronary injection of MPIO-based contrast agents, as well as injury, edema and iron-sensitive MRI. Within the first hours after I/R, we found T1 mapping to be most sensitive for tissue injury, with no changes in edema-sensitive MRI. Intriguingly, P-selectin MPIO contrast agent selectively enhanced the ischemic area in iron-sensitive MRI. In conclusion, this approach allows for sensitive detection of early myocardial inflammation beyond traditional edema-sensitive imaging.https://doi.org/10.1038/s44303-025-00069-z |
| spellingShingle | Timo Heidt Simon Reiss Julien Thielmann Christian Weber Alexander Maier Thomas Lottner Heidi R. Cristina-Schmitz Timon Bühler Diana Chiang Claus Jülicher Carolin Wadle Ingo Hilgendorf Dennis Wolf Gavin Tumlinson Luis Hortells Dirk Westermann Michael Bock Constantin von zur Mühlen Interventional real-time molecular MRI for targeting early myocardial injury in a pig model npj Imaging |
| title | Interventional real-time molecular MRI for targeting early myocardial injury in a pig model |
| title_full | Interventional real-time molecular MRI for targeting early myocardial injury in a pig model |
| title_fullStr | Interventional real-time molecular MRI for targeting early myocardial injury in a pig model |
| title_full_unstemmed | Interventional real-time molecular MRI for targeting early myocardial injury in a pig model |
| title_short | Interventional real-time molecular MRI for targeting early myocardial injury in a pig model |
| title_sort | interventional real time molecular mri for targeting early myocardial injury in a pig model |
| url | https://doi.org/10.1038/s44303-025-00069-z |
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