The study of miRNA-211 affects melanogenesis progress in Cashmere goats via suppressing <i>AP1S2</i>
<p>Chromaticity is a key indicator of measuring economic worth of Cashmere goats, and gene regulation plays a major role in Cashmere goat color. By targeting the 3<span class="inline-formula"><sup>′</sup></span>UTR region of genes, such as miRNA-211, miRNA pla...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2025-02-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/68/125/2025/aab-68-125-2025.pdf |
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| Summary: | <p>Chromaticity is a key indicator of measuring economic worth of Cashmere goats, and gene regulation plays a major role in Cashmere goat color. By targeting the 3<span class="inline-formula"><sup>′</sup></span>UTR region of genes, such as miRNA-211, miRNA plays a significant regulatory role in melanogenesis. But the mechanism of how miRNA-211 affects the melanogenesis in Cashmere goats is unclear. As a result, insights into the color regulation by miRNA-211 in Cashmere goats and its brief mechanism are offered in this study. First, the target gene <i>AP1S2</i> of miRNA-211 was screened by TargetScan and GO/KEGG, and then the targeted relationship was confirmed by the dual-luciferase reporter experiment. In B16-F10 cells, which overexpress miRNA-211 and goat skin, the opposite expression trends between <i>AP1S2</i> and miRNA-211 were analyzed by reverse transcription–quantitative PCR (RT-qPCR) and western blot. And their opposite expression trends were confirmed in B16-F10 cells by immunofluorescence. The information above showed miRNA-211 downregulated the expression of <i>AP1S2</i>. Afterwards, the impact of miRNA-211 targeting <i>AP1S2</i> on the melanogenesis of Cashmere goats was confirmed by animal regression studies. Utilizing immunohistochemistry, the expression of <i>AP1S2</i> in a mouse hair follicle was observed. Following subcutaneous injection of antagomiRNA-211 (chemical modification inhibitor), the expression levels of the <i>AP1S2</i> gene and protein were enhanced, and the localization in the hair follicle was also increased. Furthermore, reduction in melanin content in the skin was detected. These results showed that miRNA-211 significantly affected the melanogenesis progress via downregulating <i>AP1S2</i> and advantageously affected the melanin content of Cashmere goat skin.</p> |
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| ISSN: | 0003-9438 2363-9822 |