The study of miRNA-211 affects melanogenesis progress in Cashmere goats via suppressing <i>AP1S2</i>
<p>Chromaticity is a key indicator of measuring economic worth of Cashmere goats, and gene regulation plays a major role in Cashmere goat color. By targeting the 3<span class="inline-formula"><sup>′</sup></span>UTR region of genes, such as miRNA-211, miRNA pla...
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| Format: | Article |
| Language: | English |
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Copernicus Publications
2025-02-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/68/125/2025/aab-68-125-2025.pdf |
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| _version_ | 1823858488903729152 |
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| author | B. Zhang R. Liu T. Ma X. Li Y. Zhao H. Su J. Li Y. Li H. Jiang Q. Zhang |
| author_facet | B. Zhang R. Liu T. Ma X. Li Y. Zhao H. Su J. Li Y. Li H. Jiang Q. Zhang |
| author_sort | B. Zhang |
| collection | DOAJ |
| description | <p>Chromaticity is a key indicator of measuring economic worth of Cashmere goats, and gene regulation plays a major role in Cashmere goat color. By targeting the 3<span class="inline-formula"><sup>′</sup></span>UTR region of genes, such as miRNA-211, miRNA plays a significant regulatory role in melanogenesis. But the mechanism of how miRNA-211 affects the melanogenesis in Cashmere goats is unclear. As a result, insights into the color regulation by miRNA-211 in Cashmere goats and its brief mechanism are offered in this study. First, the target gene <i>AP1S2</i> of miRNA-211 was screened by TargetScan and GO/KEGG, and then the targeted relationship was confirmed by the dual-luciferase reporter experiment. In B16-F10 cells, which overexpress miRNA-211 and goat skin, the opposite expression trends between <i>AP1S2</i> and miRNA-211 were analyzed by reverse transcription–quantitative PCR (RT-qPCR) and western blot. And their opposite expression trends were confirmed in B16-F10 cells by immunofluorescence. The information above showed miRNA-211 downregulated the expression of <i>AP1S2</i>. Afterwards, the impact of miRNA-211 targeting <i>AP1S2</i> on the melanogenesis of Cashmere goats was confirmed by animal regression studies. Utilizing immunohistochemistry, the expression of <i>AP1S2</i> in a mouse hair follicle was observed. Following subcutaneous injection of antagomiRNA-211 (chemical modification inhibitor), the expression levels of the <i>AP1S2</i> gene and protein were enhanced, and the localization in the hair follicle was also increased. Furthermore, reduction in melanin content in the skin was detected. These results showed that miRNA-211 significantly affected the melanogenesis progress via downregulating <i>AP1S2</i> and advantageously affected the melanin content of Cashmere goat skin.</p> |
| format | Article |
| id | doaj-art-6f65f040b1ad47c5baa244f3c2263218 |
| institution | Kabale University |
| issn | 0003-9438 2363-9822 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Archives Animal Breeding |
| spelling | doaj-art-6f65f040b1ad47c5baa244f3c22632182025-02-11T11:08:10ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222025-02-016812513410.5194/aab-68-125-2025The study of miRNA-211 affects melanogenesis progress in Cashmere goats via suppressing <i>AP1S2</i>B. Zhang0R. Liu1T. Ma2X. Li3Y. Zhao4H. Su5J. Li6Y. Li7H. Jiang8Q. Zhang9College of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin 132109, ChinaCollege of Animal Science, Jilin University, Changchun 130062, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130033, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, China<p>Chromaticity is a key indicator of measuring economic worth of Cashmere goats, and gene regulation plays a major role in Cashmere goat color. By targeting the 3<span class="inline-formula"><sup>′</sup></span>UTR region of genes, such as miRNA-211, miRNA plays a significant regulatory role in melanogenesis. But the mechanism of how miRNA-211 affects the melanogenesis in Cashmere goats is unclear. As a result, insights into the color regulation by miRNA-211 in Cashmere goats and its brief mechanism are offered in this study. First, the target gene <i>AP1S2</i> of miRNA-211 was screened by TargetScan and GO/KEGG, and then the targeted relationship was confirmed by the dual-luciferase reporter experiment. In B16-F10 cells, which overexpress miRNA-211 and goat skin, the opposite expression trends between <i>AP1S2</i> and miRNA-211 were analyzed by reverse transcription–quantitative PCR (RT-qPCR) and western blot. And their opposite expression trends were confirmed in B16-F10 cells by immunofluorescence. The information above showed miRNA-211 downregulated the expression of <i>AP1S2</i>. Afterwards, the impact of miRNA-211 targeting <i>AP1S2</i> on the melanogenesis of Cashmere goats was confirmed by animal regression studies. Utilizing immunohistochemistry, the expression of <i>AP1S2</i> in a mouse hair follicle was observed. Following subcutaneous injection of antagomiRNA-211 (chemical modification inhibitor), the expression levels of the <i>AP1S2</i> gene and protein were enhanced, and the localization in the hair follicle was also increased. Furthermore, reduction in melanin content in the skin was detected. These results showed that miRNA-211 significantly affected the melanogenesis progress via downregulating <i>AP1S2</i> and advantageously affected the melanin content of Cashmere goat skin.</p>https://aab.copernicus.org/articles/68/125/2025/aab-68-125-2025.pdf |
| spellingShingle | B. Zhang R. Liu T. Ma X. Li Y. Zhao H. Su J. Li Y. Li H. Jiang Q. Zhang The study of miRNA-211 affects melanogenesis progress in Cashmere goats via suppressing <i>AP1S2</i> Archives Animal Breeding |
| title | The study of miRNA-211 affects melanogenesis progress in Cashmere goats via suppressing <i>AP1S2</i> |
| title_full | The study of miRNA-211 affects melanogenesis progress in Cashmere goats via suppressing <i>AP1S2</i> |
| title_fullStr | The study of miRNA-211 affects melanogenesis progress in Cashmere goats via suppressing <i>AP1S2</i> |
| title_full_unstemmed | The study of miRNA-211 affects melanogenesis progress in Cashmere goats via suppressing <i>AP1S2</i> |
| title_short | The study of miRNA-211 affects melanogenesis progress in Cashmere goats via suppressing <i>AP1S2</i> |
| title_sort | study of mirna 211 affects melanogenesis progress in cashmere goats via suppressing i ap1s2 i |
| url | https://aab.copernicus.org/articles/68/125/2025/aab-68-125-2025.pdf |
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