First detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024)
Abstract Background Jingmen tick virus (JMTV) is a novel tick-borne virus detected for the first time in Rhipicephalus (Boophilus) microplus in China. To date, there is no information regarding the circulation of JMTV in ticks collected from livestock in Cameroon. As part of the surveillance for arb...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-02-01
|
| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-025-06670-w |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825197617534992384 |
|---|---|
| author | Paloma Kiwan Eva Lopez Morena Gasparine Geraldine Piorkowski Agathe Colmant Achille Paguem Stephanie Mvodo Laurence Thirion Xavier de Lamballerie Remi Charrel Alessandra Falchi |
| author_facet | Paloma Kiwan Eva Lopez Morena Gasparine Geraldine Piorkowski Agathe Colmant Achille Paguem Stephanie Mvodo Laurence Thirion Xavier de Lamballerie Remi Charrel Alessandra Falchi |
| author_sort | Paloma Kiwan |
| collection | DOAJ |
| description | Abstract Background Jingmen tick virus (JMTV) is a novel tick-borne virus detected for the first time in Rhipicephalus (Boophilus) microplus in China. To date, there is no information regarding the circulation of JMTV in ticks collected from livestock in Cameroon. As part of the surveillance for arboviral circulation, this study aimed to assess the presence of JMTV in ticks collected from livestock (cattle and sheep) in an area of the Akonolinga health district, Center Region, Cameroon. Methods A cross sectional study was carried out during the dry season between 5 and 14 March 2024. Ticks were collected from cattle and sheep in six sampling sites in an area approximately 30 km long and 18 km wide along the Nyong River, in central Cameroon. Ticks were identified morphologically and molecularly. Total RNA/DNA was extracted from tick pools and screened for JMTV RNA using a segment 2 RT-qPCR system. Positive JMTV pools were sequenced for partial JMTV-Segment 1 and full genome analyses. Results A total of 622 ticks, organized into 251 pools were collected from 155 cattle and nine sheep. They consisted of five species covering three genera: R. (B.) microplus (472; 75.9%), Amblyomma variegatum (118; 19.0%), Hyalomma truncatum (13; 2.1%), Hyalomma rufipes (2; 0.3%), and other Rhipicephalus spp. (17; 2.7%). The quantitative reverse transcription polymerase chain reaction (qRT-PCR) screening of 251 tick pools yielded 61 JMTV-positive pools, of which 58 corresponded to R. (B.) microplus. Multiple sequence analysis revealed that JMTV from the Akonolinga area shared > 95% identity with strains from Guinea, and that these strains clustered phylogenetically together. Conclusions We provide molecular evidence of the presence of JMTV in R. (B.) microplus and A. variegatum collected from cattle and sheep from an area not yet recognized as endemic for this virus, confirming its wide geographical distribution. Graphical Abstract |
| format | Article |
| id | doaj-art-6f750536931e4a2cbd3ee5f91d82c42a |
| institution | Kabale University |
| issn | 1756-3305 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj-art-6f750536931e4a2cbd3ee5f91d82c42a2025-02-09T12:15:11ZengBMCParasites & Vectors1756-33052025-02-0118111310.1186/s13071-025-06670-wFirst detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024)Paloma Kiwan0Eva Lopez1Morena Gasparine2Geraldine Piorkowski3Agathe Colmant4Achille Paguem5Stephanie Mvodo6Laurence Thirion7Xavier de Lamballerie8Remi Charrel9Alessandra Falchi10Unite Des Virus Emergents (UVE: Aix-Marseille Univ, Universita Di Corsica, IRD 190, Inserm 1207, IRBA)Unite Des Virus Emergents (UVE: Aix-Marseille Univ, Universita Di Corsica, IRD 190, Inserm 1207, IRBA)Unite Des Virus Emergents (UVE: Aix-Marseille Univ, Universita Di Corsica, IRD 190, Inserm 1207, IRBA)Unite Des Virus Emergents (UVE: Aix-Marseille Univ, Universita Di Corsica, IRD 190, Inserm 1207, IRBA)Unite Des Virus Emergents (UVE: Aix-Marseille Univ, Universita Di Corsica, IRD 190, Inserm 1207, IRBA)Faculté d’Agriculture et de Médecine Vétérinaire, Université de BueaFaculté d’Agriculture et de Médecine Vétérinaire, Université de BueaUnite Des Virus Emergents (UVE: Aix-Marseille Univ, Universita Di Corsica, IRD 190, Inserm 1207, IRBA)Unite Des Virus Emergents (UVE: Aix-Marseille Univ, Universita Di Corsica, IRD 190, Inserm 1207, IRBA)Unite Des Virus Emergents (UVE: Aix-Marseille Univ, Universita Di Corsica, IRD 190, Inserm 1207, IRBA)Unite Des Virus Emergents (UVE: Aix-Marseille Univ, Universita Di Corsica, IRD 190, Inserm 1207, IRBA)Abstract Background Jingmen tick virus (JMTV) is a novel tick-borne virus detected for the first time in Rhipicephalus (Boophilus) microplus in China. To date, there is no information regarding the circulation of JMTV in ticks collected from livestock in Cameroon. As part of the surveillance for arboviral circulation, this study aimed to assess the presence of JMTV in ticks collected from livestock (cattle and sheep) in an area of the Akonolinga health district, Center Region, Cameroon. Methods A cross sectional study was carried out during the dry season between 5 and 14 March 2024. Ticks were collected from cattle and sheep in six sampling sites in an area approximately 30 km long and 18 km wide along the Nyong River, in central Cameroon. Ticks were identified morphologically and molecularly. Total RNA/DNA was extracted from tick pools and screened for JMTV RNA using a segment 2 RT-qPCR system. Positive JMTV pools were sequenced for partial JMTV-Segment 1 and full genome analyses. Results A total of 622 ticks, organized into 251 pools were collected from 155 cattle and nine sheep. They consisted of five species covering three genera: R. (B.) microplus (472; 75.9%), Amblyomma variegatum (118; 19.0%), Hyalomma truncatum (13; 2.1%), Hyalomma rufipes (2; 0.3%), and other Rhipicephalus spp. (17; 2.7%). The quantitative reverse transcription polymerase chain reaction (qRT-PCR) screening of 251 tick pools yielded 61 JMTV-positive pools, of which 58 corresponded to R. (B.) microplus. Multiple sequence analysis revealed that JMTV from the Akonolinga area shared > 95% identity with strains from Guinea, and that these strains clustered phylogenetically together. Conclusions We provide molecular evidence of the presence of JMTV in R. (B.) microplus and A. variegatum collected from cattle and sheep from an area not yet recognized as endemic for this virus, confirming its wide geographical distribution. Graphical Abstracthttps://doi.org/10.1186/s13071-025-06670-wJingmen tick virusTicksCattleSheepCameroon |
| spellingShingle | Paloma Kiwan Eva Lopez Morena Gasparine Geraldine Piorkowski Agathe Colmant Achille Paguem Stephanie Mvodo Laurence Thirion Xavier de Lamballerie Remi Charrel Alessandra Falchi First detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024) Parasites & Vectors Jingmen tick virus Ticks Cattle Sheep Cameroon |
| title | First detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024) |
| title_full | First detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024) |
| title_fullStr | First detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024) |
| title_full_unstemmed | First detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024) |
| title_short | First detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024) |
| title_sort | first detection and molecular characterization of jingmen tick virus with a high occurrence in rhipicephalus boophilus microplus collected from livestock in cameroon 2024 |
| topic | Jingmen tick virus Ticks Cattle Sheep Cameroon |
| url | https://doi.org/10.1186/s13071-025-06670-w |
| work_keys_str_mv | AT palomakiwan firstdetectionandmolecularcharacterizationofjingmentickviruswithahighoccurrenceinrhipicephalusboophilusmicropluscollectedfromlivestockincameroon2024 AT evalopez firstdetectionandmolecularcharacterizationofjingmentickviruswithahighoccurrenceinrhipicephalusboophilusmicropluscollectedfromlivestockincameroon2024 AT morenagasparine firstdetectionandmolecularcharacterizationofjingmentickviruswithahighoccurrenceinrhipicephalusboophilusmicropluscollectedfromlivestockincameroon2024 AT geraldinepiorkowski firstdetectionandmolecularcharacterizationofjingmentickviruswithahighoccurrenceinrhipicephalusboophilusmicropluscollectedfromlivestockincameroon2024 AT agathecolmant firstdetectionandmolecularcharacterizationofjingmentickviruswithahighoccurrenceinrhipicephalusboophilusmicropluscollectedfromlivestockincameroon2024 AT achillepaguem firstdetectionandmolecularcharacterizationofjingmentickviruswithahighoccurrenceinrhipicephalusboophilusmicropluscollectedfromlivestockincameroon2024 AT stephaniemvodo firstdetectionandmolecularcharacterizationofjingmentickviruswithahighoccurrenceinrhipicephalusboophilusmicropluscollectedfromlivestockincameroon2024 AT laurencethirion firstdetectionandmolecularcharacterizationofjingmentickviruswithahighoccurrenceinrhipicephalusboophilusmicropluscollectedfromlivestockincameroon2024 AT xavierdelamballerie firstdetectionandmolecularcharacterizationofjingmentickviruswithahighoccurrenceinrhipicephalusboophilusmicropluscollectedfromlivestockincameroon2024 AT remicharrel firstdetectionandmolecularcharacterizationofjingmentickviruswithahighoccurrenceinrhipicephalusboophilusmicropluscollectedfromlivestockincameroon2024 AT alessandrafalchi firstdetectionandmolecularcharacterizationofjingmentickviruswithahighoccurrenceinrhipicephalusboophilusmicropluscollectedfromlivestockincameroon2024 |