Comparative transcriptomic analysis and identification of candidate genes related to Verticillium wilt resistance in Gossypium barbadense and Gossypium hirsutum
The resistance to Verticillium wilt in Gossypium barbadense is generally greater than that in Gossypium hirsutum, and analyzing the differences in the mechanism and regulatory genes involved in Verticillium wilt resistance between G. barbadense and G. hirsutum is particularly important. Here, we rep...
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Elsevier
2025-03-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X25000247 |
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| author | Jieyin Zhao Xingui Hu Yuxiang Wang Jiaxin Lu Wenju Gao Xuening Su Quanjia Chen Yanying Qu |
| author_facet | Jieyin Zhao Xingui Hu Yuxiang Wang Jiaxin Lu Wenju Gao Xuening Su Quanjia Chen Yanying Qu |
| author_sort | Jieyin Zhao |
| collection | DOAJ |
| description | The resistance to Verticillium wilt in Gossypium barbadense is generally greater than that in Gossypium hirsutum, and analyzing the differences in the mechanism and regulatory genes involved in Verticillium wilt resistance between G. barbadense and G. hirsutum is particularly important. Here, we report a transcriptomic study for phenotypic evaluation of Verticillium wilt resistance in G. hirsutum (TM-1) and G. barbadense (Hai7124) and a comparison of the transcriptomes at 7 time points after Verticillium dahliae inoculation. Phenotypic evaluation revealed that, compared with TM-1, Hai7124 was more resistant to Verticillium wilt. A total of 18,138 differentially expressed genes (DEGs), including 1470 transcription factors (TFs). Further analysis of the expression of hormone biosynthesis- and signal transduction-related genes revealed that most of the genes in the salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) biosynthesis pathways were highly expressed in Hai7124; the expression of SA and JA biosynthesis genes began to be significantly upregulated in the early stage of Verticillium wilt stress, while the expression of ET biosynthesis genes was upregulated mainly in the later stage. WGCNA-based binding sequence comparison revealed that MYB14 had nonsynonymous single nucleotide polymorphisms (SNPs) in 5 highly Verticillium wilt-resistant G. barbadense varieties compared to 5 highly Verticillium wilt-susceptible G. hirsutum varieties. Expression analysis revealed that GbMYB14 responded more rapidly to Verticillium wilt stress than the same gene in G. hirsutum. The resistance of G. barbadense and G. hirsutum to Verticillium wilt decreased after MYB14 silencing via virus induced gene silencing (VIGS), and leaf yellowing and necrosis in the GbMYB14-silenced plants were more obvious. Compared with those in G. hirsutum, the expression levels and of lignin biosynthesis pathway genes and the lignin content in GbMYB14-silenced plants were lower. In conclusion, our results provide a theoretical basis for an in-depth understanding of the molecular mechanism underlying the difference in Verticillium wilt resistance between G. barbadense and G. hirsutum and provide a new genetic resource for the study of cotton resistance to Verticillium wilt. |
| format | Article |
| id | doaj-art-70f2010003a549e8b09ce04a195aeb84 |
| institution | Kabale University |
| issn | 2667-064X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-70f2010003a549e8b09ce04a195aeb842025-02-08T05:01:34ZengElsevierPlant Stress2667-064X2025-03-0115100759Comparative transcriptomic analysis and identification of candidate genes related to Verticillium wilt resistance in Gossypium barbadense and Gossypium hirsutumJieyin Zhao0Xingui Hu1Yuxiang Wang2Jiaxin Lu3Wenju Gao4Xuening Su5Quanjia Chen6Yanying Qu7Engineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi 830052, ChinaEngineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi 830052, ChinaEngineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi 830052, ChinaEngineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi 830052, ChinaEngineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi 830052, ChinaEngineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi 830052, ChinaEngineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi 830052, ChinaCorresponding author.; Engineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi 830052, ChinaThe resistance to Verticillium wilt in Gossypium barbadense is generally greater than that in Gossypium hirsutum, and analyzing the differences in the mechanism and regulatory genes involved in Verticillium wilt resistance between G. barbadense and G. hirsutum is particularly important. Here, we report a transcriptomic study for phenotypic evaluation of Verticillium wilt resistance in G. hirsutum (TM-1) and G. barbadense (Hai7124) and a comparison of the transcriptomes at 7 time points after Verticillium dahliae inoculation. Phenotypic evaluation revealed that, compared with TM-1, Hai7124 was more resistant to Verticillium wilt. A total of 18,138 differentially expressed genes (DEGs), including 1470 transcription factors (TFs). Further analysis of the expression of hormone biosynthesis- and signal transduction-related genes revealed that most of the genes in the salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) biosynthesis pathways were highly expressed in Hai7124; the expression of SA and JA biosynthesis genes began to be significantly upregulated in the early stage of Verticillium wilt stress, while the expression of ET biosynthesis genes was upregulated mainly in the later stage. WGCNA-based binding sequence comparison revealed that MYB14 had nonsynonymous single nucleotide polymorphisms (SNPs) in 5 highly Verticillium wilt-resistant G. barbadense varieties compared to 5 highly Verticillium wilt-susceptible G. hirsutum varieties. Expression analysis revealed that GbMYB14 responded more rapidly to Verticillium wilt stress than the same gene in G. hirsutum. The resistance of G. barbadense and G. hirsutum to Verticillium wilt decreased after MYB14 silencing via virus induced gene silencing (VIGS), and leaf yellowing and necrosis in the GbMYB14-silenced plants were more obvious. Compared with those in G. hirsutum, the expression levels and of lignin biosynthesis pathway genes and the lignin content in GbMYB14-silenced plants were lower. In conclusion, our results provide a theoretical basis for an in-depth understanding of the molecular mechanism underlying the difference in Verticillium wilt resistance between G. barbadense and G. hirsutum and provide a new genetic resource for the study of cotton resistance to Verticillium wilt.http://www.sciencedirect.com/science/article/pii/S2667064X25000247Gossypium hirsutumGossypium barbadenseVerticillium wiltRNA-seqCandidate genes |
| spellingShingle | Jieyin Zhao Xingui Hu Yuxiang Wang Jiaxin Lu Wenju Gao Xuening Su Quanjia Chen Yanying Qu Comparative transcriptomic analysis and identification of candidate genes related to Verticillium wilt resistance in Gossypium barbadense and Gossypium hirsutum Plant Stress Gossypium hirsutum Gossypium barbadense Verticillium wilt RNA-seq Candidate genes |
| title | Comparative transcriptomic analysis and identification of candidate genes related to Verticillium wilt resistance in Gossypium barbadense and Gossypium hirsutum |
| title_full | Comparative transcriptomic analysis and identification of candidate genes related to Verticillium wilt resistance in Gossypium barbadense and Gossypium hirsutum |
| title_fullStr | Comparative transcriptomic analysis and identification of candidate genes related to Verticillium wilt resistance in Gossypium barbadense and Gossypium hirsutum |
| title_full_unstemmed | Comparative transcriptomic analysis and identification of candidate genes related to Verticillium wilt resistance in Gossypium barbadense and Gossypium hirsutum |
| title_short | Comparative transcriptomic analysis and identification of candidate genes related to Verticillium wilt resistance in Gossypium barbadense and Gossypium hirsutum |
| title_sort | comparative transcriptomic analysis and identification of candidate genes related to verticillium wilt resistance in gossypium barbadense and gossypium hirsutum |
| topic | Gossypium hirsutum Gossypium barbadense Verticillium wilt RNA-seq Candidate genes |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X25000247 |
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