Primary Neuronal Culture and Transient Transfection
Primary neuronal culture and transient transfection offer a pair of crucial tools for neuroscience research, providing a controlled environment to study the behavior, function, and interactions of neurons in vitro. These cultures can be used to investigate fundamental aspects of neuronal development...
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2025-01-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5169&type=0 |
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| author | Shun- Tseng Peng- Chen Eric Hwang |
| author_facet | Shun- Tseng Peng- Chen Eric Hwang |
| author_sort | Shun- Tseng |
| collection | DOAJ |
| description | Primary neuronal culture and transient transfection offer a pair of crucial tools for neuroscience research, providing a controlled environment to study the behavior, function, and interactions of neurons in vitro. These cultures can be used to investigate fundamental aspects of neuronal development and plasticity, as well as disease mechanisms. There are numerous methods of transient transfection, such as electroporation, calcium phosphate precipitation, or cationic lipid transfection. In this protocol, we used electroporation for neurons immediately before plating and cationic lipid transfection for neurons that have been cultured for a few days in vitro. In our experience, the transfection efficiency of electroporation can be as high as 30%, and cationic lipid transfection has an efficiency of 1%–2%. While cationic lipid transfection has much lower efficiency than electroporation, it does offer the advantage of a higher expression level. Therefore, these transfection methods are suitable for different stages of neurons and different expression requirements. |
| format | Article |
| id | doaj-art-7133e0556a684449978936e94f4563b7 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-7133e0556a684449978936e94f4563b72025-02-07T08:16:38ZengBio-protocol LLCBio-Protocol2331-83252025-01-0115210.21769/BioProtoc.5169Primary Neuronal Culture and Transient TransfectionShun- Tseng0Peng- Chen1Eric Hwang2Department of Orthopedic Surgery, Changhua Christian Hospital, Changhua, TaiwanDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, TaiwanInstitute of Molecular Medicine and Bioengineering, National Yang Ming Chiao Tung University, Hsinchu, TaiwanDepartment of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, TaiwanInstitute of Molecular Medicine and Bioengineering, National Yang Ming Chiao Tung University, Hsinchu, Taiwan, Institute of Bioinformatics and Systems Biology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan, Center for Intelligent Drug Systems and Smart Bio-devices (IDS2B), National Yang Ming Chiao Tung University, Hsinchu, TaiwanPrimary neuronal culture and transient transfection offer a pair of crucial tools for neuroscience research, providing a controlled environment to study the behavior, function, and interactions of neurons in vitro. These cultures can be used to investigate fundamental aspects of neuronal development and plasticity, as well as disease mechanisms. There are numerous methods of transient transfection, such as electroporation, calcium phosphate precipitation, or cationic lipid transfection. In this protocol, we used electroporation for neurons immediately before plating and cationic lipid transfection for neurons that have been cultured for a few days in vitro. In our experience, the transfection efficiency of electroporation can be as high as 30%, and cationic lipid transfection has an efficiency of 1%–2%. While cationic lipid transfection has much lower efficiency than electroporation, it does offer the advantage of a higher expression level. Therefore, these transfection methods are suitable for different stages of neurons and different expression requirements.https://bio-protocol.org/en/bpdetail?id=5169&type=0 |
| spellingShingle | Shun- Tseng Peng- Chen Eric Hwang Primary Neuronal Culture and Transient Transfection Bio-Protocol |
| title | Primary Neuronal Culture and Transient Transfection |
| title_full | Primary Neuronal Culture and Transient Transfection |
| title_fullStr | Primary Neuronal Culture and Transient Transfection |
| title_full_unstemmed | Primary Neuronal Culture and Transient Transfection |
| title_short | Primary Neuronal Culture and Transient Transfection |
| title_sort | primary neuronal culture and transient transfection |
| url | https://bio-protocol.org/en/bpdetail?id=5169&type=0 |
| work_keys_str_mv | AT shuntseng primaryneuronalcultureandtransienttransfection AT pengchen primaryneuronalcultureandtransienttransfection AT erichwang primaryneuronalcultureandtransienttransfection |