CD163, a novel receptor for TNF, was revealed in situ by proximity ligation assay
Cytokine therapy utilizes cytokines to enhance the immune system to fight diseases. These strategies rely on advanced knowledge, including the communication between cytokines and their receptors. In situ, cytokine-receptor interactions are typically analyzed by co-localization using immunolabeling....
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025005742 |
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| author | Alexandre Cousin Myriam Oger Aymar de Jenlis Audrey Lejart Laure Barbier Diane Riccobono Xavier Holy Anne-Laure Favier Krisztina Nikovics |
| author_facet | Alexandre Cousin Myriam Oger Aymar de Jenlis Audrey Lejart Laure Barbier Diane Riccobono Xavier Holy Anne-Laure Favier Krisztina Nikovics |
| author_sort | Alexandre Cousin |
| collection | DOAJ |
| description | Cytokine therapy utilizes cytokines to enhance the immune system to fight diseases. These strategies rely on advanced knowledge, including the communication between cytokines and their receptors. In situ, cytokine-receptor interactions are typically analyzed by co-localization using immunolabeling. Our study compared co-localization using the Proximity Ligation Assay (PLA), a recently developed in situ protein-protein interaction technique. In an inflamed porcine lung model, we demonstrated the efficacy of PLA in detecting interactions between tumor necrosis factor (TNF) and its receptors TNFR1 and TNFR2. Additionally, the CD163 receptor was identified as a novel partner of TNF. Furthermore, the combination of immunolabeling and PLA offered additional insights, particularly, the internalization of TNF following its binding with CD163 in macrophages. Our work focused on in situ interactions of TNF with macrophages TNF receptors and suggested exciting perspectives for further understanding and application of cytokine-based therapies. |
| format | Article |
| id | doaj-art-736b85cd93ac4cb69341472042355417 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-736b85cd93ac4cb693414720423554172025-02-07T04:47:54ZengElsevierHeliyon2405-84402025-02-01113e42194CD163, a novel receptor for TNF, was revealed in situ by proximity ligation assayAlexandre Cousin0Myriam Oger1Aymar de Jenlis2Audrey Lejart3Laure Barbier4Diane Riccobono5Xavier Holy6Anne-Laure Favier7Krisztina Nikovics8Imagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223, Brétigny-sur-Orge, FranceImagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223, Brétigny-sur-Orge, FranceImagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223, Brétigny-sur-Orge, FranceMolecular Biology Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223, Brétigny-sur-Orge, FranceMolecular Biology Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223, Brétigny-sur-Orge, FranceRadiobiology Unit, Department of NRBC Defense, French Armed Forces Biomedical Research Institute, 91223, Brétigny-sur-Orge, FranceDepartment of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223, Brétigny-sur-Orge, FranceImagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223, Brétigny-sur-Orge, FranceImagery Unit, Department of Platforms and Technology Research, French Armed Forces Biomedical Research Institute, 91223, Brétigny-sur-Orge, France; Corresponding author.Cytokine therapy utilizes cytokines to enhance the immune system to fight diseases. These strategies rely on advanced knowledge, including the communication between cytokines and their receptors. In situ, cytokine-receptor interactions are typically analyzed by co-localization using immunolabeling. Our study compared co-localization using the Proximity Ligation Assay (PLA), a recently developed in situ protein-protein interaction technique. In an inflamed porcine lung model, we demonstrated the efficacy of PLA in detecting interactions between tumor necrosis factor (TNF) and its receptors TNFR1 and TNFR2. Additionally, the CD163 receptor was identified as a novel partner of TNF. Furthermore, the combination of immunolabeling and PLA offered additional insights, particularly, the internalization of TNF following its binding with CD163 in macrophages. Our work focused on in situ interactions of TNF with macrophages TNF receptors and suggested exciting perspectives for further understanding and application of cytokine-based therapies.http://www.sciencedirect.com/science/article/pii/S2405844025005742TNFCD163InflammationRegenerationProximity ligation assaySPR |
| spellingShingle | Alexandre Cousin Myriam Oger Aymar de Jenlis Audrey Lejart Laure Barbier Diane Riccobono Xavier Holy Anne-Laure Favier Krisztina Nikovics CD163, a novel receptor for TNF, was revealed in situ by proximity ligation assay Heliyon TNF CD163 Inflammation Regeneration Proximity ligation assay SPR |
| title | CD163, a novel receptor for TNF, was revealed in situ by proximity ligation assay |
| title_full | CD163, a novel receptor for TNF, was revealed in situ by proximity ligation assay |
| title_fullStr | CD163, a novel receptor for TNF, was revealed in situ by proximity ligation assay |
| title_full_unstemmed | CD163, a novel receptor for TNF, was revealed in situ by proximity ligation assay |
| title_short | CD163, a novel receptor for TNF, was revealed in situ by proximity ligation assay |
| title_sort | cd163 a novel receptor for tnf was revealed in situ by proximity ligation assay |
| topic | TNF CD163 Inflammation Regeneration Proximity ligation assay SPR |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025005742 |
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