The protein interactome of Escherichia coli carbohydrate metabolism.
We investigate how protein-protein interactions (PPIs) can regulate carbohydrate metabolism in Escherichia coli. We specifically investigated the stoichiometry of 378 PPIs involving carbohydrate metabolic enzymes. In 48 interactions, the interactors were much more abundant than the enzyme and are th...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0315240 |
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| author | Shomeek Chowdhury Stephen S Fong Peter Uetz |
| author_facet | Shomeek Chowdhury Stephen S Fong Peter Uetz |
| author_sort | Shomeek Chowdhury |
| collection | DOAJ |
| description | We investigate how protein-protein interactions (PPIs) can regulate carbohydrate metabolism in Escherichia coli. We specifically investigated the stoichiometry of 378 PPIs involving carbohydrate metabolic enzymes. In 48 interactions, the interactors were much more abundant than the enzyme and are thus likely to affect enzyme activity and carbohydrate metabolism. Many of these PPIs are conserved across thousands of bacteria including pathogens and microbial species. E. coli adapts to different cellular environments by adjusting the quantities of the interacting proteins (25 PPIs) in a way that the protein-enzyme interaction (PEI) is a likely mechanism to regulate its metabolism in specific environments. We predict 3 PPIs (RpsB-AdhE, DcyD-NanE and MinE-Yccx) previously not known to regulate metabolism. |
| format | Article |
| id | doaj-art-7433496376ff4f99b6d2e0a9c1453ade |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-7433496376ff4f99b6d2e0a9c1453ade2025-02-09T05:30:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e031524010.1371/journal.pone.0315240The protein interactome of Escherichia coli carbohydrate metabolism.Shomeek ChowdhuryStephen S FongPeter UetzWe investigate how protein-protein interactions (PPIs) can regulate carbohydrate metabolism in Escherichia coli. We specifically investigated the stoichiometry of 378 PPIs involving carbohydrate metabolic enzymes. In 48 interactions, the interactors were much more abundant than the enzyme and are thus likely to affect enzyme activity and carbohydrate metabolism. Many of these PPIs are conserved across thousands of bacteria including pathogens and microbial species. E. coli adapts to different cellular environments by adjusting the quantities of the interacting proteins (25 PPIs) in a way that the protein-enzyme interaction (PEI) is a likely mechanism to regulate its metabolism in specific environments. We predict 3 PPIs (RpsB-AdhE, DcyD-NanE and MinE-Yccx) previously not known to regulate metabolism.https://doi.org/10.1371/journal.pone.0315240 |
| spellingShingle | Shomeek Chowdhury Stephen S Fong Peter Uetz The protein interactome of Escherichia coli carbohydrate metabolism. PLoS ONE |
| title | The protein interactome of Escherichia coli carbohydrate metabolism. |
| title_full | The protein interactome of Escherichia coli carbohydrate metabolism. |
| title_fullStr | The protein interactome of Escherichia coli carbohydrate metabolism. |
| title_full_unstemmed | The protein interactome of Escherichia coli carbohydrate metabolism. |
| title_short | The protein interactome of Escherichia coli carbohydrate metabolism. |
| title_sort | protein interactome of escherichia coli carbohydrate metabolism |
| url | https://doi.org/10.1371/journal.pone.0315240 |
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