PLIN5 contributes to lipophagy of hepatic stellate cells induced by inorganic arsenic
Arsenic exposure triggers the activation of hepatic stellate cells (HSCs), resulting in liver fibrosis (LF). A significant decrease in lipid droplets marks the activation of HSCs. However, the exact underlying molecular mechanism remains elusive. Lipophagy, a specialized form of selective autophagy,...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324016233 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856911790899200 |
|---|---|
| author | Haomiao Li Xuri Xie Tianming Qiu Jingyuan Zhang Jie Bai Guang Yang Ningning Wang Xiaofeng Yao Xiance Sun |
| author_facet | Haomiao Li Xuri Xie Tianming Qiu Jingyuan Zhang Jie Bai Guang Yang Ningning Wang Xiaofeng Yao Xiance Sun |
| author_sort | Haomiao Li |
| collection | DOAJ |
| description | Arsenic exposure triggers the activation of hepatic stellate cells (HSCs), resulting in liver fibrosis (LF). A significant decrease in lipid droplets marks the activation of HSCs. However, the exact underlying molecular mechanism remains elusive. Lipophagy, a specialized form of selective autophagy, is crucial for the degradation of lipid droplets to maintain intracellular lipid metabolism homeostasis. In this study, arsenic treatment induced lipophagy, as evidenced by the co-localization of LC3 with lipid droplets. Remarkably, arsenic exposure increased the expression levels of Perilipin 5 (PLIN5), a lipid droplet-associated protein, both at the mRNA and protein levels. Moreover, silencing PLIN5 influenced arsenic-induced lipolysis. Consequently, the results of this study indicate that PLIN5 serves as a substrate protein involved in arsenic-induced lipophagy. This research offers a novel perspective on the mechanisms of arsenic-induced HSCs activation and liver lipid metabolism, potentially guiding new strategies for the prevention and treatment of arsenic-related liver diseases. |
| format | Article |
| id | doaj-art-76c55bf80de5436c8cac9157a5e8da9b |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-76c55bf80de5436c8cac9157a5e8da9b2025-02-12T05:29:38ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117547PLIN5 contributes to lipophagy of hepatic stellate cells induced by inorganic arsenicHaomiao Li0Xuri Xie1Tianming Qiu2Jingyuan Zhang3Jie Bai4Guang Yang5Ningning Wang6Xiaofeng Yao7Xiance Sun8Department of Occupational and Environmental Health, School of Public Health, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, PR ChinaDepartment of Occupational and Environmental Health, School of Public Health, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, PR ChinaDepartment of Occupational and Environmental Health, School of Public Health, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, PR ChinaDepartment of Occupational and Environmental Health, School of Public Health, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, PR ChinaDepartment of Public Health Experimental Teaching Center, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, PR ChinaDepartment of Nutrition and Food Safety, School of Public Health, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, PR ChinaDepartment of Public Health Experimental Teaching Center, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, PR China; Global Health Research Center, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, PR ChinaDepartment of Occupational and Environmental Health, School of Public Health, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, PR ChinaDepartment of Occupational and Environmental Health, School of Public Health, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, PR China; Global Health Research Center, Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, PR China; Correspondence to: Department of Occupational and Environmental Health, Global Health Research Center, School of Public Health, Dalian Medical University, Dalian 166044, PR China.Arsenic exposure triggers the activation of hepatic stellate cells (HSCs), resulting in liver fibrosis (LF). A significant decrease in lipid droplets marks the activation of HSCs. However, the exact underlying molecular mechanism remains elusive. Lipophagy, a specialized form of selective autophagy, is crucial for the degradation of lipid droplets to maintain intracellular lipid metabolism homeostasis. In this study, arsenic treatment induced lipophagy, as evidenced by the co-localization of LC3 with lipid droplets. Remarkably, arsenic exposure increased the expression levels of Perilipin 5 (PLIN5), a lipid droplet-associated protein, both at the mRNA and protein levels. Moreover, silencing PLIN5 influenced arsenic-induced lipolysis. Consequently, the results of this study indicate that PLIN5 serves as a substrate protein involved in arsenic-induced lipophagy. This research offers a novel perspective on the mechanisms of arsenic-induced HSCs activation and liver lipid metabolism, potentially guiding new strategies for the prevention and treatment of arsenic-related liver diseases.http://www.sciencedirect.com/science/article/pii/S0147651324016233ArsenicPerilipin 5LipophagyHSCs |
| spellingShingle | Haomiao Li Xuri Xie Tianming Qiu Jingyuan Zhang Jie Bai Guang Yang Ningning Wang Xiaofeng Yao Xiance Sun PLIN5 contributes to lipophagy of hepatic stellate cells induced by inorganic arsenic Ecotoxicology and Environmental Safety Arsenic Perilipin 5 Lipophagy HSCs |
| title | PLIN5 contributes to lipophagy of hepatic stellate cells induced by inorganic arsenic |
| title_full | PLIN5 contributes to lipophagy of hepatic stellate cells induced by inorganic arsenic |
| title_fullStr | PLIN5 contributes to lipophagy of hepatic stellate cells induced by inorganic arsenic |
| title_full_unstemmed | PLIN5 contributes to lipophagy of hepatic stellate cells induced by inorganic arsenic |
| title_short | PLIN5 contributes to lipophagy of hepatic stellate cells induced by inorganic arsenic |
| title_sort | plin5 contributes to lipophagy of hepatic stellate cells induced by inorganic arsenic |
| topic | Arsenic Perilipin 5 Lipophagy HSCs |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324016233 |
| work_keys_str_mv | AT haomiaoli plin5contributestolipophagyofhepaticstellatecellsinducedbyinorganicarsenic AT xurixie plin5contributestolipophagyofhepaticstellatecellsinducedbyinorganicarsenic AT tianmingqiu plin5contributestolipophagyofhepaticstellatecellsinducedbyinorganicarsenic AT jingyuanzhang plin5contributestolipophagyofhepaticstellatecellsinducedbyinorganicarsenic AT jiebai plin5contributestolipophagyofhepaticstellatecellsinducedbyinorganicarsenic AT guangyang plin5contributestolipophagyofhepaticstellatecellsinducedbyinorganicarsenic AT ningningwang plin5contributestolipophagyofhepaticstellatecellsinducedbyinorganicarsenic AT xiaofengyao plin5contributestolipophagyofhepaticstellatecellsinducedbyinorganicarsenic AT xiancesun plin5contributestolipophagyofhepaticstellatecellsinducedbyinorganicarsenic |