Facile colorimetric detection of As(V) in Rice with immobilized acid phosphatase on hollow metal-organic frameworks hybrid with peroxidase-like activity
Quantitative analysis of As(V) in rice is of great significance for food safety and heavy metal pollution control. Here, a facile colorimetric method for As(V) detection was constructed by using immobilized acid phosphatase (ACP) in hollow metal-organic frameworks hybrid. Metalloporphyrin and gold n...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524009398 |
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| author | Zhiyang Qin Zhuolan Xu Yixin Liu Xinguang Qin Gang Liu Xinlin Wei Haizhi Zhang |
| author_facet | Zhiyang Qin Zhuolan Xu Yixin Liu Xinguang Qin Gang Liu Xinlin Wei Haizhi Zhang |
| author_sort | Zhiyang Qin |
| collection | DOAJ |
| description | Quantitative analysis of As(V) in rice is of great significance for food safety and heavy metal pollution control. Here, a facile colorimetric method for As(V) detection was constructed by using immobilized acid phosphatase (ACP) in hollow metal-organic frameworks hybrid. Metalloporphyrin and gold nanoparticles modified hollow zeolite imidazole framework-8 [Au/HZIF-8@TCPP(Fe)], named AuHT, was chosen here as ACP immobilizing carrier with peroxidase-like activity. Firstly, the morphology, structure, immobilization efficiency and catalytic ability of obtained AuHT@ACP were fully characterized. Then, based on the inhibition of As(V) on immobilized ACP and cascade reaction mediated by ACP and AuHT, a colorimetric biosensor was established with excellent simplicity. After comprehensive validation, this colorimetric method presented the advantages of wide linear range (10.0–1000.0 μg/L), low LOD (4.0 μg/L), nice accuracy (recovery of 93.7–109.6 %) and good selectivity. Finally, this method was applied to visual detection of As(V) in rice samples with different varieties. |
| format | Article |
| id | doaj-art-7735f5cc33984081a3affbf8012efd60 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-7735f5cc33984081a3affbf8012efd602025-02-12T05:31:48ZengElsevierFood Chemistry: X2590-15752025-01-0125102051Facile colorimetric detection of As(V) in Rice with immobilized acid phosphatase on hollow metal-organic frameworks hybrid with peroxidase-like activityZhiyang Qin0Zhuolan Xu1Yixin Liu2Xinguang Qin3Gang Liu4Xinlin Wei5Haizhi Zhang6College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaCollege of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaCollege of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaCollege of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaCollege of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaCollege of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaCorresponding author at: Xuefu South Road No.68, Changqing Garden, Wuhan City, Hubei Province 430023, China.; College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, ChinaQuantitative analysis of As(V) in rice is of great significance for food safety and heavy metal pollution control. Here, a facile colorimetric method for As(V) detection was constructed by using immobilized acid phosphatase (ACP) in hollow metal-organic frameworks hybrid. Metalloporphyrin and gold nanoparticles modified hollow zeolite imidazole framework-8 [Au/HZIF-8@TCPP(Fe)], named AuHT, was chosen here as ACP immobilizing carrier with peroxidase-like activity. Firstly, the morphology, structure, immobilization efficiency and catalytic ability of obtained AuHT@ACP were fully characterized. Then, based on the inhibition of As(V) on immobilized ACP and cascade reaction mediated by ACP and AuHT, a colorimetric biosensor was established with excellent simplicity. After comprehensive validation, this colorimetric method presented the advantages of wide linear range (10.0–1000.0 μg/L), low LOD (4.0 μg/L), nice accuracy (recovery of 93.7–109.6 %) and good selectivity. Finally, this method was applied to visual detection of As(V) in rice samples with different varieties.http://www.sciencedirect.com/science/article/pii/S2590157524009398As(V)Colorimetric detectionAcid phosphataseNanozymeHollow MOF |
| spellingShingle | Zhiyang Qin Zhuolan Xu Yixin Liu Xinguang Qin Gang Liu Xinlin Wei Haizhi Zhang Facile colorimetric detection of As(V) in Rice with immobilized acid phosphatase on hollow metal-organic frameworks hybrid with peroxidase-like activity Food Chemistry: X As(V) Colorimetric detection Acid phosphatase Nanozyme Hollow MOF |
| title | Facile colorimetric detection of As(V) in Rice with immobilized acid phosphatase on hollow metal-organic frameworks hybrid with peroxidase-like activity |
| title_full | Facile colorimetric detection of As(V) in Rice with immobilized acid phosphatase on hollow metal-organic frameworks hybrid with peroxidase-like activity |
| title_fullStr | Facile colorimetric detection of As(V) in Rice with immobilized acid phosphatase on hollow metal-organic frameworks hybrid with peroxidase-like activity |
| title_full_unstemmed | Facile colorimetric detection of As(V) in Rice with immobilized acid phosphatase on hollow metal-organic frameworks hybrid with peroxidase-like activity |
| title_short | Facile colorimetric detection of As(V) in Rice with immobilized acid phosphatase on hollow metal-organic frameworks hybrid with peroxidase-like activity |
| title_sort | facile colorimetric detection of as v in rice with immobilized acid phosphatase on hollow metal organic frameworks hybrid with peroxidase like activity |
| topic | As(V) Colorimetric detection Acid phosphatase Nanozyme Hollow MOF |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524009398 |
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