Knockdown of PTEN promotes colon cancer progression and induces M2 macrophage polarization in the colon cancer cell environment
Objective: This article aims to study the effect of phosphate and tension homolog deleted on chromosome ten (PTEN) knockdown on colon cancer progression and macrophage polarization in the cancer environment. Materials and Methods and Results: The expression of PTEN in colon cancer tissues and colon...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-07-01
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| Series: | Indian Journal of Pathology and Microbiology |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/ijpm.ijpm_786_21 |
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| _version_ | 1825206161948803072 |
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| author | Xu Han Ting Yan Lina Wang Bin He Huaxu Yu |
| author_facet | Xu Han Ting Yan Lina Wang Bin He Huaxu Yu |
| author_sort | Xu Han |
| collection | DOAJ |
| description | Objective:
This article aims to study the effect of phosphate and tension homolog deleted on chromosome ten (PTEN) knockdown on colon cancer progression and macrophage polarization in the cancer environment.
Materials and Methods and Results:
The expression of PTEN in colon cancer tissues and colon cancer cells was significantly lower than in precancerous tissues or CCD-18Co cells, and the decrease was most evident in SW620 cells. The expressions of phosphate (p)-p38, c-Jun N-terminal kinase (JNK), activator protein 1 (AP-1), B-cell lymphoma-2 (Bcl-2) protein in colon cancer tissues and cells were significantly higher than in precancerous tissues or CCD-18Co cells (P-values < 0.05). Bcl-2-associated X (Bax) and Caspase-3 expressions in colon cancer tissues and cells were significantly lower than in precancerous tissues or CCD-18Co cells (P-values < 0.05). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was applied to measure cell viability. Transwell evaluated the cell migration and invasion ability. Si-PTEN improved the proliferation, migration, and invasion of SW620 cells (P-values < 0.05). The expression levels of arginase-1 (Arg-1), CD163, CD206 in colon cancer tissues were significantly higher than in precancerous tissues (P-values < 0.05). The cell cycle, the number of M1 and M2 double-positive cells were assessed by flow cytometry. Si-PTEN reduced the expression of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and inducible nitric oxide synthase (iNOS), which upregulated the expression of Arg-1, CD206, CD163, p-p38, JNK, and AP-1 (P-values < 0.05).
Conclusion:
Si-PTEN promoted colon cancer progression and induced the polarization of M2 tumor-associated macrophages in the colon cancer cell environment. |
| format | Article |
| id | doaj-art-790d84d15442447f8aa5858d3d07c8eb |
| institution | Kabale University |
| issn | 0377-4929 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Pathology and Microbiology |
| spelling | doaj-art-790d84d15442447f8aa5858d3d07c8eb2025-02-07T13:30:18ZengWolters Kluwer Medknow PublicationsIndian Journal of Pathology and Microbiology0377-49292023-07-0166347848710.4103/ijpm.ijpm_786_21Knockdown of PTEN promotes colon cancer progression and induces M2 macrophage polarization in the colon cancer cell environmentXu HanTing YanLina WangBin HeHuaxu YuObjective: This article aims to study the effect of phosphate and tension homolog deleted on chromosome ten (PTEN) knockdown on colon cancer progression and macrophage polarization in the cancer environment. Materials and Methods and Results: The expression of PTEN in colon cancer tissues and colon cancer cells was significantly lower than in precancerous tissues or CCD-18Co cells, and the decrease was most evident in SW620 cells. The expressions of phosphate (p)-p38, c-Jun N-terminal kinase (JNK), activator protein 1 (AP-1), B-cell lymphoma-2 (Bcl-2) protein in colon cancer tissues and cells were significantly higher than in precancerous tissues or CCD-18Co cells (P-values < 0.05). Bcl-2-associated X (Bax) and Caspase-3 expressions in colon cancer tissues and cells were significantly lower than in precancerous tissues or CCD-18Co cells (P-values < 0.05). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was applied to measure cell viability. Transwell evaluated the cell migration and invasion ability. Si-PTEN improved the proliferation, migration, and invasion of SW620 cells (P-values < 0.05). The expression levels of arginase-1 (Arg-1), CD163, CD206 in colon cancer tissues were significantly higher than in precancerous tissues (P-values < 0.05). The cell cycle, the number of M1 and M2 double-positive cells were assessed by flow cytometry. Si-PTEN reduced the expression of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), and inducible nitric oxide synthase (iNOS), which upregulated the expression of Arg-1, CD206, CD163, p-p38, JNK, and AP-1 (P-values < 0.05). Conclusion: Si-PTEN promoted colon cancer progression and induced the polarization of M2 tumor-associated macrophages in the colon cancer cell environment.https://journals.lww.com/10.4103/ijpm.ijpm_786_21colon cancermacrophage m2 polarizationpten |
| spellingShingle | Xu Han Ting Yan Lina Wang Bin He Huaxu Yu Knockdown of PTEN promotes colon cancer progression and induces M2 macrophage polarization in the colon cancer cell environment Indian Journal of Pathology and Microbiology colon cancer macrophage m2 polarization pten |
| title | Knockdown of PTEN promotes colon cancer progression and induces M2 macrophage polarization in the colon cancer cell environment |
| title_full | Knockdown of PTEN promotes colon cancer progression and induces M2 macrophage polarization in the colon cancer cell environment |
| title_fullStr | Knockdown of PTEN promotes colon cancer progression and induces M2 macrophage polarization in the colon cancer cell environment |
| title_full_unstemmed | Knockdown of PTEN promotes colon cancer progression and induces M2 macrophage polarization in the colon cancer cell environment |
| title_short | Knockdown of PTEN promotes colon cancer progression and induces M2 macrophage polarization in the colon cancer cell environment |
| title_sort | knockdown of pten promotes colon cancer progression and induces m2 macrophage polarization in the colon cancer cell environment |
| topic | colon cancer macrophage m2 polarization pten |
| url | https://journals.lww.com/10.4103/ijpm.ijpm_786_21 |
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