Bioinformatics screening and clinical validation of CircRNA and related miRNA in male osteoporosis
Abstract Background The pathogenesis of male osteoporosis (MOP) remains unclear, with the role of genetic factors attracting the attention of researchers. In the present study, we aimed to investigate critical circRNA biomarkers associated with male osteoporosis. Methods RNA-sequencing was performed...
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BMC
2025-02-01
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| Series: | BMC Musculoskeletal Disorders |
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| Online Access: | https://doi.org/10.1186/s12891-024-08171-w |
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| author | Jiayi Li Sijia Guo Qingyun Sun Ning An Jisheng Lin Qi Fei |
| author_facet | Jiayi Li Sijia Guo Qingyun Sun Ning An Jisheng Lin Qi Fei |
| author_sort | Jiayi Li |
| collection | DOAJ |
| description | Abstract Background The pathogenesis of male osteoporosis (MOP) remains unclear, with the role of genetic factors attracting the attention of researchers. In the present study, we aimed to investigate critical circRNA biomarkers associated with male osteoporosis. Methods RNA-sequencing was performed to investigate the circRNA expression profiles between 3 men with osteoporosis and 3 with normal mass density. Then, shared mRNAs between host genes acquired in this present study and mRNAs acquired in previous study were identified to screen vital circRNAs associated with male osteoporosis. PPI networks of shared mRNAs were constructed and the hub genes in the PPI networks were identified with CytoHubba, a plugin in Cytoscape software (3.10.1). Finally, a ceRNA network of four circRNAs derived from three hub genes was constructed. Validation experiments were performed on selected circRNAs and related miRNAs in this ceRNA network using peripheral blood clinical samples. Results In total, 657 circRNAs were detected in male osteoporosis. The shared mRNAs were significantly enriched in the metabolic pathways, RNA transport, Ubiquitin mediated proteolysis and Amyotrophic lateral sclerosis. Then, three genes, including SETD2, ATM and XPO1, were identified as hub genes with four algorithms. Ultimately, the ceRNA network, involving 4 circRNAs, 40 miRNAs, and 592 mRNAs, was obtained. Using 35 clinical samples, three potential circRNAs and three miRNAs associated with male osteoporosis were selected for validation. It was ultimately found that three miRNAs were upregulated in MOP, while hsa-circ-9130, novel_circ_0014940 and hsa-circ-0054894 were upregulated, hsa-circ-2484 and novel_circ_0033084 were downregulated in patients with MOP. Conclusion We emphasized the roles of several significantly up- and down-regulated circRNAs and four circRNAs derived from three hub genes in male osteoporosis. Differences in expression were confirmed for three miRNAs and five circRNAs in the ceRNA network among patients with male osteoporosis. |
| format | Article |
| id | doaj-art-7a2ce4b0feaa478fb14ca4e6677bbfd4 |
| institution | Kabale University |
| issn | 1471-2474 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj-art-7a2ce4b0feaa478fb14ca4e6677bbfd42025-02-09T12:04:21ZengBMCBMC Musculoskeletal Disorders1471-24742025-02-0126111210.1186/s12891-024-08171-wBioinformatics screening and clinical validation of CircRNA and related miRNA in male osteoporosisJiayi Li0Sijia Guo1Qingyun Sun2Ning An3Jisheng Lin4Qi Fei5Department of Orthopedics, Beijing Friendship Hospital, Capital Medical UniversityDepartment of Orthopedics, Beijing Friendship Hospital, Capital Medical UniversityDepartment of Orthopedics, Beijing Friendship Hospital, Capital Medical UniversityDepartment of Orthopedics, Beijing Friendship Hospital, Capital Medical UniversityDepartment of Orthopedics, Beijing Friendship Hospital, Capital Medical UniversityDepartment of Orthopedics, Beijing Friendship Hospital, Capital Medical UniversityAbstract Background The pathogenesis of male osteoporosis (MOP) remains unclear, with the role of genetic factors attracting the attention of researchers. In the present study, we aimed to investigate critical circRNA biomarkers associated with male osteoporosis. Methods RNA-sequencing was performed to investigate the circRNA expression profiles between 3 men with osteoporosis and 3 with normal mass density. Then, shared mRNAs between host genes acquired in this present study and mRNAs acquired in previous study were identified to screen vital circRNAs associated with male osteoporosis. PPI networks of shared mRNAs were constructed and the hub genes in the PPI networks were identified with CytoHubba, a plugin in Cytoscape software (3.10.1). Finally, a ceRNA network of four circRNAs derived from three hub genes was constructed. Validation experiments were performed on selected circRNAs and related miRNAs in this ceRNA network using peripheral blood clinical samples. Results In total, 657 circRNAs were detected in male osteoporosis. The shared mRNAs were significantly enriched in the metabolic pathways, RNA transport, Ubiquitin mediated proteolysis and Amyotrophic lateral sclerosis. Then, three genes, including SETD2, ATM and XPO1, were identified as hub genes with four algorithms. Ultimately, the ceRNA network, involving 4 circRNAs, 40 miRNAs, and 592 mRNAs, was obtained. Using 35 clinical samples, three potential circRNAs and three miRNAs associated with male osteoporosis were selected for validation. It was ultimately found that three miRNAs were upregulated in MOP, while hsa-circ-9130, novel_circ_0014940 and hsa-circ-0054894 were upregulated, hsa-circ-2484 and novel_circ_0033084 were downregulated in patients with MOP. Conclusion We emphasized the roles of several significantly up- and down-regulated circRNAs and four circRNAs derived from three hub genes in male osteoporosis. Differences in expression were confirmed for three miRNAs and five circRNAs in the ceRNA network among patients with male osteoporosis.https://doi.org/10.1186/s12891-024-08171-wMale osteoporosiscircRNAHost geneceRNA |
| spellingShingle | Jiayi Li Sijia Guo Qingyun Sun Ning An Jisheng Lin Qi Fei Bioinformatics screening and clinical validation of CircRNA and related miRNA in male osteoporosis BMC Musculoskeletal Disorders Male osteoporosis circRNA Host gene ceRNA |
| title | Bioinformatics screening and clinical validation of CircRNA and related miRNA in male osteoporosis |
| title_full | Bioinformatics screening and clinical validation of CircRNA and related miRNA in male osteoporosis |
| title_fullStr | Bioinformatics screening and clinical validation of CircRNA and related miRNA in male osteoporosis |
| title_full_unstemmed | Bioinformatics screening and clinical validation of CircRNA and related miRNA in male osteoporosis |
| title_short | Bioinformatics screening and clinical validation of CircRNA and related miRNA in male osteoporosis |
| title_sort | bioinformatics screening and clinical validation of circrna and related mirna in male osteoporosis |
| topic | Male osteoporosis circRNA Host gene ceRNA |
| url | https://doi.org/10.1186/s12891-024-08171-w |
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