Shedding light on the unseen: how live imaging of translation could unlock new insights in developmental biology
Recent advances in live imaging technologies have refined our understanding of protein synthesis in living cells. Among the various approaches to live imaging of translation, this perspective highlights the use of antibody-based nascent peptide detection, a method that enables visualization of singl...
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| Format: | Article |
| Language: | English |
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Académie des sciences
2024-09-01
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| Series: | Comptes Rendus Biologies |
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| Online Access: | https://comptes-rendus.academie-sciences.fr/biologies/articles/10.5802/crbiol.158/ |
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| _version_ | 1825206310652608512 |
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| author | Dufourt, Jeremy Bellec, Maelle |
| author_facet | Dufourt, Jeremy Bellec, Maelle |
| author_sort | Dufourt, Jeremy |
| collection | DOAJ |
| description | Recent advances in live imaging technologies have refined our understanding of protein synthesis in living cells. Among the various approaches to live imaging of translation, this perspective highlights the use of antibody-based nascent peptide detection, a method that enables visualization of single-molecule translation in vivo. We examine how these advances improve our understanding of biological processes, particularly in developing organisms. In addition, we discuss technological advances in this field and suggest further improvements. Finally, we review some examples of how this method could lead to future scientific breakthroughs in the study of translation and its regulation in whole organisms. |
| format | Article |
| id | doaj-art-7bab18a8fd9a4d65b0b24663b3e16e71 |
| institution | Kabale University |
| issn | 1768-3238 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Académie des sciences |
| record_format | Article |
| series | Comptes Rendus Biologies |
| spelling | doaj-art-7bab18a8fd9a4d65b0b24663b3e16e712025-02-07T10:37:47ZengAcadémie des sciencesComptes Rendus Biologies1768-32382024-09-01347G1879310.5802/crbiol.15810.5802/crbiol.158Shedding light on the unseen: how live imaging of translation could unlock new insights in developmental biologyDufourt, Jeremy0https://orcid.org/0000-0003-4043-7250Bellec, Maelle1https://orcid.org/0000-0002-9029-0042Institut de Recherche en Infectiologie de Montpellier, CNRS UMR 9004, Univ Montpellier, 1919 Route de Mende, Montpellier, 34293, Cedex 5, FranceDepartment of Developmental Genetics, Max Planck Institute for Heart and Lung Research, Bad Nauheim, GermanyRecent advances in live imaging technologies have refined our understanding of protein synthesis in living cells. Among the various approaches to live imaging of translation, this perspective highlights the use of antibody-based nascent peptide detection, a method that enables visualization of single-molecule translation in vivo. We examine how these advances improve our understanding of biological processes, particularly in developing organisms. In addition, we discuss technological advances in this field and suggest further improvements. Finally, we review some examples of how this method could lead to future scientific breakthroughs in the study of translation and its regulation in whole organisms.https://comptes-rendus.academie-sciences.fr/biologies/articles/10.5802/crbiol.158/TranslationLive-imagingDevelopment |
| spellingShingle | Dufourt, Jeremy Bellec, Maelle Shedding light on the unseen: how live imaging of translation could unlock new insights in developmental biology Comptes Rendus Biologies Translation Live-imaging Development |
| title | Shedding light on the unseen: how live imaging of translation could unlock new insights in developmental biology |
| title_full | Shedding light on the unseen: how live imaging of translation could unlock new insights in developmental biology |
| title_fullStr | Shedding light on the unseen: how live imaging of translation could unlock new insights in developmental biology |
| title_full_unstemmed | Shedding light on the unseen: how live imaging of translation could unlock new insights in developmental biology |
| title_short | Shedding light on the unseen: how live imaging of translation could unlock new insights in developmental biology |
| title_sort | shedding light on the unseen how live imaging of translation could unlock new insights in developmental biology |
| topic | Translation Live-imaging Development |
| url | https://comptes-rendus.academie-sciences.fr/biologies/articles/10.5802/crbiol.158/ |
| work_keys_str_mv | AT dufourtjeremy sheddinglightontheunseenhowliveimagingoftranslationcouldunlocknewinsightsindevelopmentalbiology AT bellecmaelle sheddinglightontheunseenhowliveimagingoftranslationcouldunlocknewinsightsindevelopmentalbiology |