Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles/miR-497 targeting colorectal cancer
Abstract Colorectal cancer (CRC) is a serious public health concern worldwide. Immune checkpoint inhibition medication is likely to remain a crucial part of CRC clinical management. This study aims to create new super paramagnetic iron oxide nano-carrier (SPION) that can effectively transport miRNA...
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Nature Portfolio
2025-02-01
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| Online Access: | https://doi.org/10.1038/s41598-025-88165-3 |
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| author | Asmaa M. Elfiky May M. Eid May El-Manawaty Zeinab A. Elshahid Elham Mohamed Youssef Khaled Mahmoud |
| author_facet | Asmaa M. Elfiky May M. Eid May El-Manawaty Zeinab A. Elshahid Elham Mohamed Youssef Khaled Mahmoud |
| author_sort | Asmaa M. Elfiky |
| collection | DOAJ |
| description | Abstract Colorectal cancer (CRC) is a serious public health concern worldwide. Immune checkpoint inhibition medication is likely to remain a crucial part of CRC clinical management. This study aims to create new super paramagnetic iron oxide nano-carrier (SPION) that can effectively transport miRNA to specific CRC cell lines. In addition, evaluate the efficiency of this nano-formulation as a therapeutic candidate for CRC. Bioinformatics tools were used to select a promising tumor suppressor miRNA (mir-497-5p). Green route, using Fusarium oxyporium fungal species, manipulated for the synthesis of SPION@Ag@Cs nanocomposite as a carrier of miR-497-5p. That specifically targets the suppression of PD1/PDL1 and CTLA4pathways for colorectal therapy. UV/visible and FTIR spectroscopy, Zeta potential and MTT were used to confirm the allocation of the miR-497 on SPION@Ag@Cs and its cytotoxicity against CRC cell lines. Immunofluorescence was employed to confirm transfection of cells with miR-497@NPs, and the down- regulation of CTLA4 in HT29, and Caco2 cell lines. On the other hand, PDL1 showed a significant increase in colorectal cell lines (HT-29 and Caco-2) in response to mir497-5p@Nano treatment. The data suggest that the mir-497 -loaded SPION@Ag@Cs nano-formulation could be a good candidate for the suppression of CTLA4in CRC human cell lines. Consequently, the targeting miR-497/CTLA4 axis is a potential immunotherapy treatment strategy for CRC. |
| format | Article |
| id | doaj-art-7c4fe1557c9949d89b7b969bff317ffb |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-7c4fe1557c9949d89b7b969bff317ffb2025-02-09T12:33:42ZengNature PortfolioScientific Reports2045-23222025-02-0115111310.1038/s41598-025-88165-3Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles/miR-497 targeting colorectal cancerAsmaa M. Elfiky0May M. Eid1May El-Manawaty2Zeinab A. Elshahid3Elham Mohamed Youssef4Khaled Mahmoud5Environmental and Occupational Medicine Department, Environment and Climate Change Research Institute, National Research CentrePhysics Institute, National Research CenterPharmaceutical Sciences Institute, Department of Pharmacognosy, National Research CentreChemistry of Natural and Microbial Products, Pharmaceutical Industry Research Institute, National Research CentreBiochemistry Department, National Research CentrePharmaceutical Sciences Institute, Department of Pharmacognosy, National Research CentreAbstract Colorectal cancer (CRC) is a serious public health concern worldwide. Immune checkpoint inhibition medication is likely to remain a crucial part of CRC clinical management. This study aims to create new super paramagnetic iron oxide nano-carrier (SPION) that can effectively transport miRNA to specific CRC cell lines. In addition, evaluate the efficiency of this nano-formulation as a therapeutic candidate for CRC. Bioinformatics tools were used to select a promising tumor suppressor miRNA (mir-497-5p). Green route, using Fusarium oxyporium fungal species, manipulated for the synthesis of SPION@Ag@Cs nanocomposite as a carrier of miR-497-5p. That specifically targets the suppression of PD1/PDL1 and CTLA4pathways for colorectal therapy. UV/visible and FTIR spectroscopy, Zeta potential and MTT were used to confirm the allocation of the miR-497 on SPION@Ag@Cs and its cytotoxicity against CRC cell lines. Immunofluorescence was employed to confirm transfection of cells with miR-497@NPs, and the down- regulation of CTLA4 in HT29, and Caco2 cell lines. On the other hand, PDL1 showed a significant increase in colorectal cell lines (HT-29 and Caco-2) in response to mir497-5p@Nano treatment. The data suggest that the mir-497 -loaded SPION@Ag@Cs nano-formulation could be a good candidate for the suppression of CTLA4in CRC human cell lines. Consequently, the targeting miR-497/CTLA4 axis is a potential immunotherapy treatment strategy for CRC.https://doi.org/10.1038/s41598-025-88165-3Colorectal cancerSPION@Ag@Cs nano-carriermiR-497-5pPD1/PDL1CTLA-4 oncogenes |
| spellingShingle | Asmaa M. Elfiky May M. Eid May El-Manawaty Zeinab A. Elshahid Elham Mohamed Youssef Khaled Mahmoud Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles/miR-497 targeting colorectal cancer Scientific Reports Colorectal cancer SPION@Ag@Cs nano-carrier miR-497-5p PD1/PDL1 CTLA-4 oncogenes |
| title | Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles/miR-497 targeting colorectal cancer |
| title_full | Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles/miR-497 targeting colorectal cancer |
| title_fullStr | Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles/miR-497 targeting colorectal cancer |
| title_full_unstemmed | Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles/miR-497 targeting colorectal cancer |
| title_short | Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles/miR-497 targeting colorectal cancer |
| title_sort | production of novel theranostic nano vector based on superparamagnetic iron oxide nanoparticles mir 497 targeting colorectal cancer |
| topic | Colorectal cancer SPION@Ag@Cs nano-carrier miR-497-5p PD1/PDL1 CTLA-4 oncogenes |
| url | https://doi.org/10.1038/s41598-025-88165-3 |
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