Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness

Aiming to enable online freshness-monitoring of meat within modified-atmosphere package, we developed a ratiometric array that was fluorescently responsive to volatile organic compounds-ammonia (NH3) released by protein decaying. The array was consisted of two 3 mm × 6 mm rectangles precisely and un...

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Main Authors: Zhijian Wang, Xudong Shi, Jingze Guo, Lin Wang, Meilin Cao, Shiyao Wang, Yisheng Chen
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Food Chemistry: X
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2590157524009878
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author Zhijian Wang
Xudong Shi
Jingze Guo
Lin Wang
Meilin Cao
Shiyao Wang
Yisheng Chen
author_facet Zhijian Wang
Xudong Shi
Jingze Guo
Lin Wang
Meilin Cao
Shiyao Wang
Yisheng Chen
author_sort Zhijian Wang
collection DOAJ
description Aiming to enable online freshness-monitoring of meat within modified-atmosphere package, we developed a ratiometric array that was fluorescently responsive to volatile organic compounds-ammonia (NH3) released by protein decaying. The array was consisted of two 3 mm × 6 mm rectangles precisely and uniformly printed with fluorescein isothiocyanate (FITC) as indicator and rhodamine B (RhB) as internal reference on the filter-paper, respectively. The fluorescence intensity of the array area was calibrated according to Green/Red ratio of the digitalized pixels extracted from images facilitated by a smartphone. The fluorescence-ratiometric sensor array displayed remarkable detection performances, including high sensitivity (LOD = 1.1 ppm), stability (91 % responding attenuation over 10 d of storage) and reproducibility (RSD < 10 %), which was further validated with real pork and shrimp samples. Subsequently, the fluorescent signals of the dual-rectangle array showed high correlation to the total volatile base nitrogen value that was officially used for indexing the meat freshness status.
format Article
id doaj-art-7c71063184c341ca88c12e5878604cd1
institution Kabale University
issn 2590-1575
language English
publishDate 2025-01-01
publisher Elsevier
record_format Article
series Food Chemistry: X
spelling doaj-art-7c71063184c341ca88c12e5878604cd12025-02-12T05:31:57ZengElsevierFood Chemistry: X2590-15752025-01-0125102099Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshnessZhijian Wang0Xudong Shi1Jingze Guo2Lin Wang3Meilin Cao4Shiyao Wang5Yisheng Chen6College of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCollege of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaCorresponding author.; College of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, ChinaAiming to enable online freshness-monitoring of meat within modified-atmosphere package, we developed a ratiometric array that was fluorescently responsive to volatile organic compounds-ammonia (NH3) released by protein decaying. The array was consisted of two 3 mm × 6 mm rectangles precisely and uniformly printed with fluorescein isothiocyanate (FITC) as indicator and rhodamine B (RhB) as internal reference on the filter-paper, respectively. The fluorescence intensity of the array area was calibrated according to Green/Red ratio of the digitalized pixels extracted from images facilitated by a smartphone. The fluorescence-ratiometric sensor array displayed remarkable detection performances, including high sensitivity (LOD = 1.1 ppm), stability (91 % responding attenuation over 10 d of storage) and reproducibility (RSD < 10 %), which was further validated with real pork and shrimp samples. Subsequently, the fluorescent signals of the dual-rectangle array showed high correlation to the total volatile base nitrogen value that was officially used for indexing the meat freshness status.http://www.sciencedirect.com/science/article/pii/S2590157524009878Fluorescence-ratiometric sensor arraySmart printingMeat freshness
spellingShingle Zhijian Wang
Xudong Shi
Jingze Guo
Lin Wang
Meilin Cao
Shiyao Wang
Yisheng Chen
Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness
Food Chemistry: X
Fluorescence-ratiometric sensor array
Smart printing
Meat freshness
title Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness
title_full Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness
title_fullStr Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness
title_full_unstemmed Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness
title_short Drop-on-demand printing of amine-responsive fluorescence-ratiometric sensor array for online monitoring meat freshness
title_sort drop on demand printing of amine responsive fluorescence ratiometric sensor array for online monitoring meat freshness
topic Fluorescence-ratiometric sensor array
Smart printing
Meat freshness
url http://www.sciencedirect.com/science/article/pii/S2590157524009878
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AT jingzeguo dropondemandprintingofamineresponsivefluorescenceratiometricsensorarrayforonlinemonitoringmeatfreshness
AT linwang dropondemandprintingofamineresponsivefluorescenceratiometricsensorarrayforonlinemonitoringmeatfreshness
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