Morphological and molecular characterization of Stenocranus pacificus (Hemiptera: Delphacidae) on maize plants

Maize (Zea mays L.) is one of the primary food commodities with a strategic role in improving Indonesia's economy. Additionally, maize contributes to food diversification and security. One of the main threats to maize production is pest attacks, including Stenocranus pacificus and Peregrinus m...

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Main Authors: Anivea Fachmi, Dita Megasari, Ramadhani Mahendra Kusuma
Format: Article
Language:English
Published: Universitas Lancang Kuning 2024-11-01
Series:Jurnal Ilmiah Pertanian
Subjects:
Online Access:https://dev-journal.unilak.ac.id/index.php/jip/article/view/20215
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author Anivea Fachmi
Dita Megasari
Ramadhani Mahendra Kusuma
author_facet Anivea Fachmi
Dita Megasari
Ramadhani Mahendra Kusuma
author_sort Anivea Fachmi
collection DOAJ
description Maize (Zea mays L.) is one of the primary food commodities with a strategic role in improving Indonesia's economy. Additionally, maize contributes to food diversification and security. One of the main threats to maize production is pest attacks, including Stenocranus pacificus and Peregrinus maidis. This study aims to identify S. pacificus (Hemiptera: Delphacidae) found on maize crops in three sub-districts of Lamongan Regency, East Java, using both morphological and molecular approaches. Morphological identification revealed that the planthopper found is S. pacificus, characterized by an orange abdomen in male imagos and a white abdomen in female imagos. Molecular testing through polymerase chain reaction (PCR) using mitochondrial Cytochrome Oxidase I deoxyribonucleic acid (mtCOI DNA) showed that the S. pacificus from Lamongan is 99.53% similar to S. pacificus WRJJ_UNILA and WRJB_UNILA. These findings are expected to support more accurate, rapid, and effective pest control strategies.
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spelling doaj-art-7cd4234748104a5c9380e7eb141c43472025-02-07T09:32:16ZengUniversitas Lancang KuningJurnal Ilmiah Pertanian1829-83462502-59882024-11-01213Morphological and molecular characterization of Stenocranus pacificus (Hemiptera: Delphacidae) on maize plants Anivea Fachmi0Dita Megasari1Ramadhani Mahendra Kusuma2Department of Agrotechnology, Faculty of Agriculture, UPN “Veteran” Jawa Timur, Surabaya 60294, Indonesia Department of Agrotechnology, Faculty of Agriculture, UPN “Veteran” Jawa Timur, Surabaya 60294, Indonesia Department of Agrotechnology, Faculty of Agriculture, UPN “Veteran” Jawa Timur, Surabaya 60294, Indonesia Maize (Zea mays L.) is one of the primary food commodities with a strategic role in improving Indonesia's economy. Additionally, maize contributes to food diversification and security. One of the main threats to maize production is pest attacks, including Stenocranus pacificus and Peregrinus maidis. This study aims to identify S. pacificus (Hemiptera: Delphacidae) found on maize crops in three sub-districts of Lamongan Regency, East Java, using both morphological and molecular approaches. Morphological identification revealed that the planthopper found is S. pacificus, characterized by an orange abdomen in male imagos and a white abdomen in female imagos. Molecular testing through polymerase chain reaction (PCR) using mitochondrial Cytochrome Oxidase I deoxyribonucleic acid (mtCOI DNA) showed that the S. pacificus from Lamongan is 99.53% similar to S. pacificus WRJJ_UNILA and WRJB_UNILA. These findings are expected to support more accurate, rapid, and effective pest control strategies. https://dev-journal.unilak.ac.id/index.php/jip/article/view/20215DNA alignmentDNA amplificationelectrophoresisphylogenetic treeStenocranus pacificus
spellingShingle Anivea Fachmi
Dita Megasari
Ramadhani Mahendra Kusuma
Morphological and molecular characterization of Stenocranus pacificus (Hemiptera: Delphacidae) on maize plants
Jurnal Ilmiah Pertanian
DNA alignment
DNA amplification
electrophoresis
phylogenetic tree
Stenocranus pacificus
title Morphological and molecular characterization of Stenocranus pacificus (Hemiptera: Delphacidae) on maize plants
title_full Morphological and molecular characterization of Stenocranus pacificus (Hemiptera: Delphacidae) on maize plants
title_fullStr Morphological and molecular characterization of Stenocranus pacificus (Hemiptera: Delphacidae) on maize plants
title_full_unstemmed Morphological and molecular characterization of Stenocranus pacificus (Hemiptera: Delphacidae) on maize plants
title_short Morphological and molecular characterization of Stenocranus pacificus (Hemiptera: Delphacidae) on maize plants
title_sort morphological and molecular characterization of stenocranus pacificus hemiptera delphacidae on maize plants
topic DNA alignment
DNA amplification
electrophoresis
phylogenetic tree
Stenocranus pacificus
url https://dev-journal.unilak.ac.id/index.php/jip/article/view/20215
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AT ramadhanimahendrakusuma morphologicalandmolecularcharacterizationofstenocranuspacificushemipteradelphacidaeonmaizeplants