Effect of eDNA metabarcoding temporal sampling strategies on detection of coastal biodiversity
IntroductionEnvironmental DNA (eDNA) metabarcoding of water is increasingly being used to monitor coastal biodiversity shifts. However, we have limited knowledge of whether samples collected during discreet temporal periods depict holistic ecosystem changes over longer time spans.MethodsHere, we sho...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2025.1522677/full |
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| author | Maelle Sevellec Anaïs Lacoursière-Roussel Eric Normandeau Louis Bernatchez Kimberly Lynn Howland |
| author_facet | Maelle Sevellec Anaïs Lacoursière-Roussel Eric Normandeau Louis Bernatchez Kimberly Lynn Howland |
| author_sort | Maelle Sevellec |
| collection | DOAJ |
| description | IntroductionEnvironmental DNA (eDNA) metabarcoding of water is increasingly being used to monitor coastal biodiversity shifts. However, we have limited knowledge of whether samples collected during discreet temporal periods depict holistic ecosystem changes over longer time spans.MethodsHere, we show how eDNA community structure varies across repeated sampling events at different temporal scales ranging from years to months to days at an Arctic coastal site, Churchill (Canada), using metabarcoding analyses of water eDNA samples with four universal primer pairs (two primers in COI and two in the 18S rRNA).ResultsDaily variations were highly dynamic and less structured, likely due to the stochastic nature of estuarine ecosystems, but there was a clear annual consistency in eDNA communities with a high proportion of shared taxa between years. However, monthly sampling was the most efficient for capturing holistic biodiversity.DiscussionWe provide recommendations for optimal eDNA metabarcoding sampling design based on our observations. The study underscores the importance of understanding biological and physical factors altering eDNA detection to improve the efficiency of detecting and interpreting long-term eDNA changes. |
| format | Article |
| id | doaj-art-7dc0da503bc44de699bc5f3d624a4cde |
| institution | Kabale University |
| issn | 2296-7745 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Marine Science |
| spelling | doaj-art-7dc0da503bc44de699bc5f3d624a4cde2025-02-07T09:45:56ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452025-02-011210.3389/fmars.2025.15226771522677Effect of eDNA metabarcoding temporal sampling strategies on detection of coastal biodiversityMaelle Sevellec0Anaïs Lacoursière-Roussel1Eric Normandeau2Louis Bernatchez3Kimberly Lynn Howland4Arctic and Aquatic Research Division, Freshwater Institute, Fisheries and Oceans Canada, Winnipeg, MB, CanadaSt. Andrews Biological Station (SABS), Fisheries and Oceans Canada, St. Andrews, NB, CanadaPlateforme de bio-informatique de l’IBIS (Institut de Biologie Intégrative et des Systèmes), Université Laval, Québec, QC, CanadaInstitut de Biologie Intégrative des Systèmes (IBIS), Université Laval, Québec, QC, CanadaArctic and Aquatic Research Division, Freshwater Institute, Fisheries and Oceans Canada, Winnipeg, MB, CanadaIntroductionEnvironmental DNA (eDNA) metabarcoding of water is increasingly being used to monitor coastal biodiversity shifts. However, we have limited knowledge of whether samples collected during discreet temporal periods depict holistic ecosystem changes over longer time spans.MethodsHere, we show how eDNA community structure varies across repeated sampling events at different temporal scales ranging from years to months to days at an Arctic coastal site, Churchill (Canada), using metabarcoding analyses of water eDNA samples with four universal primer pairs (two primers in COI and two in the 18S rRNA).ResultsDaily variations were highly dynamic and less structured, likely due to the stochastic nature of estuarine ecosystems, but there was a clear annual consistency in eDNA communities with a high proportion of shared taxa between years. However, monthly sampling was the most efficient for capturing holistic biodiversity.DiscussionWe provide recommendations for optimal eDNA metabarcoding sampling design based on our observations. The study underscores the importance of understanding biological and physical factors altering eDNA detection to improve the efficiency of detecting and interpreting long-term eDNA changes.https://www.frontiersin.org/articles/10.3389/fmars.2025.1522677/fullArcticeDNA metabarcodingtemporal sampling strategieseDNA annual recurrenceeDNA monthly biodiversityeDNA daily variation |
| spellingShingle | Maelle Sevellec Anaïs Lacoursière-Roussel Eric Normandeau Louis Bernatchez Kimberly Lynn Howland Effect of eDNA metabarcoding temporal sampling strategies on detection of coastal biodiversity Frontiers in Marine Science Arctic eDNA metabarcoding temporal sampling strategies eDNA annual recurrence eDNA monthly biodiversity eDNA daily variation |
| title | Effect of eDNA metabarcoding temporal sampling strategies on detection of coastal biodiversity |
| title_full | Effect of eDNA metabarcoding temporal sampling strategies on detection of coastal biodiversity |
| title_fullStr | Effect of eDNA metabarcoding temporal sampling strategies on detection of coastal biodiversity |
| title_full_unstemmed | Effect of eDNA metabarcoding temporal sampling strategies on detection of coastal biodiversity |
| title_short | Effect of eDNA metabarcoding temporal sampling strategies on detection of coastal biodiversity |
| title_sort | effect of edna metabarcoding temporal sampling strategies on detection of coastal biodiversity |
| topic | Arctic eDNA metabarcoding temporal sampling strategies eDNA annual recurrence eDNA monthly biodiversity eDNA daily variation |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2025.1522677/full |
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