Histological and immune response in the fish Centropomus viridis elicited by the parasite Rhabdosynochus viridisi
Objective. To analyze histological and immunological changes in the Pacific white snook (PWS) Centropomus viridis during primary infection and re-infection with the monogenean Rhabdosynochus viridisi. Materials and methods. Samplings were performed at three timepoints (0,1, and 2). Histological alt...
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Universidad de Cordoba
2024-07-01
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| Series: | Revista MVZ Cordoba |
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| Online Access: | https://revistamvz.unicordoba.edu.co/article/view/3381 |
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| author | Dania López-Moreno Zeinab Yazdi Francisco N. Morales-Serna Juan M. Martínez-Brown Leonardo Ibarra-Castro Alejandra García-Gasca Selene M. Abad-Rosales Rodolfo Lozano-Olvera Emma J. Fajer-Ávila Esteban Soto |
| author_facet | Dania López-Moreno Zeinab Yazdi Francisco N. Morales-Serna Juan M. Martínez-Brown Leonardo Ibarra-Castro Alejandra García-Gasca Selene M. Abad-Rosales Rodolfo Lozano-Olvera Emma J. Fajer-Ávila Esteban Soto |
| author_sort | Dania López-Moreno |
| collection | DOAJ |
| description |
Objective. To analyze histological and immunological changes in the Pacific white snook (PWS) Centropomus viridis during primary infection and re-infection with the monogenean Rhabdosynochus viridisi. Materials and methods. Samplings were performed at three timepoints (0,1, and 2). Histological alterations in gills were evaluated by the severity degree and the degree of tissue change. RT-qPCR assays were developed to investigate the expression of il1B, il8, il10, il12, il17, igM, igT, ifnγ, tnfα, tbet, hsp70, foxp3a, stat4, and cmip in gills and head kidney. Results. The prevalence of infection was 100% in challenged fish. During the primary infection, the mean intensity was 152 parasites per fish at Time 1 and 94 at Time 2, while in the reinfection, it was 367 parasites at Time 1 and 129 at Time 2. Histological analysis of gills showed fusion of the secondary lamellae, hyperplasia, infiltration of mononuclear inflammatory cells, and increase of chloride cells in both primary infections and reinfections. Only the expression of cmip in gills at Time 1 was significantly higher in reinfections than in primary infections, and the expression of il12β showed a fold-change value >100 in head kidney at Time 2 in primary infections. Conclusions. The monogenean R. viridisi may cause histological alteration in its fish host. As showed by the decrease of the intensity of infection from Time 1 to Time 2, it seems that the PWS is able to combat R. viridisi; however, our immunological analysis did not reveal strong evidence of a possible mechanism.
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| format | Article |
| id | doaj-art-7dd470dd30614edcbd1031567e4c2d04 |
| institution | Kabale University |
| issn | 0122-0268 1909-0544 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Universidad de Cordoba |
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| series | Revista MVZ Cordoba |
| spelling | doaj-art-7dd470dd30614edcbd1031567e4c2d042025-02-11T17:17:09ZengUniversidad de CordobaRevista MVZ Cordoba0122-02681909-05442024-07-0129210.21897/rmvz.3381Histological and immune response in the fish Centropomus viridis elicited by the parasite Rhabdosynochus viridisiDania López-Moreno0https://orcid.org/0000-0001-9979-3642Zeinab Yazdi1https://orcid.org/0000-0003-3533-912XFrancisco N. Morales-Serna2https://orcid.org/0000-0002-2577-5369Juan M. Martínez-Brown3https://orcid.org/0000-0002-9647-7481Leonardo Ibarra-Castro4https://orcid.org/0000-0002-2159-9038Alejandra García-Gasca5https://orcid.org/0000-0002-4496-2434Selene M. Abad-Rosales6https://orcid.org/0000-0002-3836-7479Rodolfo Lozano-Olvera7https://orcid.org/0000-0002-9650-1525Emma J. Fajer-Ávila8https://orcid.org/0000-0003-3230-5709Esteban Soto9https://orcid.org/0000-0001-6054-9634Centro de Investigación en Alimentación y Desarrollo, MéxicoUniversidad de California, Estados UnidosUniversidad Nacional Autónoma de MéxicoCentro de Investigación en Alimentación y Desarrollo, MéxicoUniversidad de la Florida, Estados UnidosCentro de Investigación en Alimentación y Desarrollo, MéxicoCentro de Investigación en Alimentación y Desarrollo, MéxicoCentro de Investigación en Alimentación y Desarrollo, MéxicoCentro de Investigación en Alimentación y Desarrollo, MéxicoUniversidad de California, Estados Unidos Objective. To analyze histological and immunological changes in the Pacific white snook (PWS) Centropomus viridis during primary infection and re-infection with the monogenean Rhabdosynochus viridisi. Materials and methods. Samplings were performed at three timepoints (0,1, and 2). Histological alterations in gills were evaluated by the severity degree and the degree of tissue change. RT-qPCR assays were developed to investigate the expression of il1B, il8, il10, il12, il17, igM, igT, ifnγ, tnfα, tbet, hsp70, foxp3a, stat4, and cmip in gills and head kidney. Results. The prevalence of infection was 100% in challenged fish. During the primary infection, the mean intensity was 152 parasites per fish at Time 1 and 94 at Time 2, while in the reinfection, it was 367 parasites at Time 1 and 129 at Time 2. Histological analysis of gills showed fusion of the secondary lamellae, hyperplasia, infiltration of mononuclear inflammatory cells, and increase of chloride cells in both primary infections and reinfections. Only the expression of cmip in gills at Time 1 was significantly higher in reinfections than in primary infections, and the expression of il12β showed a fold-change value >100 in head kidney at Time 2 in primary infections. Conclusions. The monogenean R. viridisi may cause histological alteration in its fish host. As showed by the decrease of the intensity of infection from Time 1 to Time 2, it seems that the PWS is able to combat R. viridisi; however, our immunological analysis did not reveal strong evidence of a possible mechanism. https://revistamvz.unicordoba.edu.co/article/view/3381AquacultureImmunologyPlatyhelminthesMonogeneaEctoparasiteCentropomidae |
| spellingShingle | Dania López-Moreno Zeinab Yazdi Francisco N. Morales-Serna Juan M. Martínez-Brown Leonardo Ibarra-Castro Alejandra García-Gasca Selene M. Abad-Rosales Rodolfo Lozano-Olvera Emma J. Fajer-Ávila Esteban Soto Histological and immune response in the fish Centropomus viridis elicited by the parasite Rhabdosynochus viridisi Revista MVZ Cordoba Aquaculture Immunology Platyhelminthes Monogenea Ectoparasite Centropomidae |
| title | Histological and immune response in the fish Centropomus viridis elicited by the parasite Rhabdosynochus viridisi |
| title_full | Histological and immune response in the fish Centropomus viridis elicited by the parasite Rhabdosynochus viridisi |
| title_fullStr | Histological and immune response in the fish Centropomus viridis elicited by the parasite Rhabdosynochus viridisi |
| title_full_unstemmed | Histological and immune response in the fish Centropomus viridis elicited by the parasite Rhabdosynochus viridisi |
| title_short | Histological and immune response in the fish Centropomus viridis elicited by the parasite Rhabdosynochus viridisi |
| title_sort | histological and immune response in the fish centropomus viridis elicited by the parasite rhabdosynochus viridisi |
| topic | Aquaculture Immunology Platyhelminthes Monogenea Ectoparasite Centropomidae |
| url | https://revistamvz.unicordoba.edu.co/article/view/3381 |
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